Organism : Bacillus cereus ATCC14579 | Module List:
Module 514 Profile

GeneModule member RegulatorRegulator MotifMotif
Cytoscape Web
Network Help

A network view of the module is created using cytoscapeWeb and enables dynamic, interactive exploration of the module properties. In this view, module member genes, motifs, and regulatory influences are represented as peripheral nodes connected to core module node via edges.

Module members are green circles, regulators are red triangles and motifs are blue diamonds. Selection of a node gives access to detailed information in a pop-up window, which allows dragging and pinning to compare multiple selections. Selecting module members will show information about the selected gene such as name, species and fucntions. Motif selection will show motif logo image and e-values. Bicluster selction will show expression profile and summary statistics for the module.

GeneModule member RegulatorRegulator MotifMotif
Regulators for Module 514

There are 10 regulatory influences for Module 514

Regulator Table (10)
Regulator Name Type
BC1427 tf
BC4076 tf
BC5352 tf
BC3320 tf
BC2401 tf
BC0405 tf
BC1851 tf
BC5385 tf
BC0840 tf
BC0742 tf

Regulator Help

For each module, single or AND logic connected regulatory influences are listed under the regulators tab. These regulatory influences are identified by Inferelator. Table shows name of the regulator and its type.

tf: Transcription factor

ef: Environmental factor

combiner: Combinatorial influence of a tf or an ef through logic gate. Table is sortable by clicking on the arrows next to column headers.

Motif information (de novo identified motifs for modules)

There are 2 motifs predicted.

Motif Table (2)
Motif Id e-value Consensus Motif Logo
4938 2.50e-08 GCtaATcgGAaAAaccaaAGGca
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4939 1.50e-01 cAGGGG.A
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Motif Help

Transcription factor binding motifs help to elucidate regulatory mechanism. cMonkey integrates powerful de novo motif detection to identify conditionally co-regulated sets of genes. De novo predicted motifs for each module are listed in the module page as motif logo images along with associated prediction statistics (e-values). The main module page also shows the location of these motifs within the upstream sequences of the module member genes.

Motifs of interest can be broadcasted to RegPredict (currently only available for Desulfovibrio vulgaris Hildenborough) in order to compare conservation in similar species. This integrated motif prediction and comparative analysis provides an additional checkpoint for regulatory motif prediction confidence.

Motif e-value: cMonkey tries to identify two motifs per modules in the upstream sequences of the module member genes. Motif e-value is an indicative of the motif co-occurences between the members of the module.Smaller e-values are indicative of significant sequence motifs. Our experience showed that e-values smaller than 10 are generally indicative of significant motifs.

Functional Enrichment

Regulon 514 is enriched for following functions.

KEGG Enrichment Table

Function Name Function Type Unadjusted pvalue Benjamini Hochberg pvalue Genes with function Method
Energy Metabolism kegg subcategory 2.74e-04 2.45e-03 4/22
Sulfur metabolism kegg pathway 0.00e+00 8.00e-06 3/22
Environmental Information Processing kegg category 0.00e+00 0.00e+00 15/22
Membrane Transport kegg subcategory 0.00e+00 0.00e+00 15/22
ABC transporters kegg pathway 0.00e+00 0.00e+00 15/22
Microbial metabolism in diverse environments kegg pathway 1.58e-03 7.44e-03 4/22

TIGRFam Enrichment Table

Function Name Function Type Unadjusted pvalue Benjamini& Hochberg pvalue Genes with function Method
oligo_HPY tigrfam 0.00e+00 0.00e+00 4/22
Central intermediary metabolism tigr mainrole 2.00e-06 5.00e-06 3/22
Sulfur metabolism tigr sub1role 0.00e+00 0.00e+00 3/22
Transport and binding proteins tigr mainrole 0.00e+00 0.00e+00 7/22
Amino acids, peptides and amines tigr sub1role 0.00e+00 1.00e-06 4/22

COG Enrichment Table

Function Name Function Type Unadjusted pvalue Benjamini& Hochberg pvalue Genes with function Method
Metabolism cog category 0.00e+00 0.00e+00 19/22
Amino acid transport and metabolism cog subcategory 0.00e+00 0.00e+00 11/22
Inorganic ion transport and metabolism cog subcategory 0.00e+00 0.00e+00 15/22
ABC-type dipeptide/oligopeptide/nickel transport system, ATPase component cog 0.00e+00 0.00e+00 4/22
Functions Help

Biological networks contain sets of regulatory units called functional modules that together play a role in regulation of specific functional processes. Connections between different modules in the network can help identify regulatory relationships such as hierarchy and epistasis. In addition, associating functions with modules enables putative assignment of functions to hypothetical genes. It is therefore essential to identify functional enrichment of modules within the regulatory network.

Functional annotations from single sources are often either not available or not complete. Therefore, we integrated KEGG pathway, Gene Ontology, TIGRFam and COG information as references for functional enrichment analysis.

We use hypergeometric p-values to identify significant overlaps between co-regulated module members and genes assigned to a particular functional annotation category. P-values are corrected for multiple comparisons by using Benjamini-Hochberg correction and filtered for p-values ≤ 0.05.

Network Portal presents functional ontologies from KEGG, GO, TIGRFAM, and COG as separate tables that include function name, type, corrected and uncorrected hypergeometric p-values, and the number of genes assigned to this category out of total number of genes in the module.

Members for Module 514

There are 22 genes in Module 514

Gene Member Table (22)
Name Common name Type Gene ID Chromosome Start End Strand Description TF
BC0241 BC0241 CDS None chromosome 216229 217956 + Oligopeptide-binding protein oppA (NCBI ptt file) False
BC0242 BC0242 CDS None chromosome 218084 219016 + Oligopeptide transport system permease protein oppB (NCBI ptt file) False
BC0243 BC0243 CDS None chromosome 219029 219949 + Oligopeptide transport system permease protein oppC (NCBI ptt file) False
BC0244 BC0244 CDS None chromosome 219960 220940 + Oligopeptide transport ATP-binding protein oppD (NCBI ptt file) False
BC0245 BC0245 CDS None chromosome 220937 221902 + Oligopeptide transport ATP-binding protein oppF (NCBI ptt file) False
BC0907 BC0907 CDS None chromosome 895869 897266 + Oligopeptide-binding protein oppA (NCBI ptt file) False
BC0908 BC0908 CDS None chromosome 897322 898272 + Oligopeptide transport system permease protein oppB (NCBI ptt file) False
BC0909 BC0909 CDS None chromosome 898279 899130 + Oligopeptide transport system permease protein oppC (NCBI ptt file) False
BC0910 BC0910 CDS None chromosome 899157 900149 + Oligopeptide transport ATP-binding protein oppD (NCBI ptt file) False
BC0911 BC0911 CDS None chromosome 900133 901113 + Oligopeptide transport ATP-binding protein oppF (NCBI ptt file) False
BC1090 BC1090 CDS None chromosome 1075838 1076404 + Sulfate-binding protein (NCBI ptt file) False
BC1093 BC1093 CDS None chromosome 1077765 1078622 + Sulfate transport system permease protein cysW (NCBI ptt file) False
BC1421 BC1421 CDS None chromosome 1382453 1383157 + Phosphoadenosine phosphosulfate reductase (NCBI ptt file) False
BC1422 BC1422 CDS None chromosome 1383197 1384333 + Sulfate adenylyltransferase (NCBI ptt file) False
BC1423 BC1423 CDS None chromosome 1384346 1384939 + Adenylylsulfate kinase (NCBI ptt file) False
BC1424 BC1424 CDS None chromosome 1384951 1386573 + Ferredoxin--nitrite reductase (NCBI ptt file) False
BC1425 BC1425 CDS None chromosome 1386584 1386784 + hypothetical protein (NCBI ptt file) False
BC1426 BC1426 CDS None chromosome 1386866 1387645 + Uroporphyrin-III C-methyltransferase (NCBI ptt file) False
BC2910 BC2910 CDS None chromosome 2867695 2868543 - Alkanesulfonates transport system permease protein (NCBI ptt file) False
BC2911 BC2911 CDS None chromosome 2868560 2869546 - Alkanesulfonates-binding protein (NCBI ptt file) False
BC2912 BC2912 CDS None chromosome 2869564 2870319 - Alkanesulfonates transport ATP-binding protein (NCBI ptt file) False
BC3050 BC3050 CDS None chromosome 3007972 3008874 + hypothetical Membrane Spanning Protein (NCBI ptt file) False

Genes Help

Gene member table shows all the genes included in the module. Listed attributes are;

  1. Name: Gene name or Locus tag
  2. Common Name: Gene short name
  3. Type: Type of the feature, usually CDS.
  4. Gene ID: Link to NCBI Gene ID
  5. Chromosome: Chromosome name from annotation file
  6. Start/End:Feature start and end coordinates
  7. Strand: strand of the gene
  8. Description: Description of the gene from annotation file
  9. TF: If the gene is a Transcription Factor or not.

If you are browsing the Network Portal by using Gaggle/Firegoose, firegoose plugin will capture the NameList of the gene members. Captured names can be saved into your Workspace by clicking on "Capture" in the firegoose toolbar or can be directly sent other desktop and web resources by using "Broadcast" option.

Comments for Module 514

Please add your comments for this module by using the form below. Your comments will be publicly available.

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Social Help

You can start a conversation about this module or join the existing discussion by adding your comments. In order to be able to add your comments you need to sign in by using any of the following services;Disqus, Google, Facebook or Twitter. For full compatibility with other network portal features, we recommend using your Google ID.

Help

What is a module?

Regulatory units (modules) in the Network Portal are based on the network inference algorithm used. For the current version, modules are based on cMonkey modules and Inferelator regulatory influences on these modules. More specifically, module refers to set of genes that are conditionally co-regulated under subset of the conditions. Identification of modules integrates co-expression, de-novo motif identification, and other functional associations such as operon information and protein-protein interactions.

Module Overview

The landing module page shows quick summary info including co-expression profiles, de-novo identified motifs, and transcription factors and/or environmental factors as regulatory influences. It also includes module residual, motif e-values, conditions and links to other resources such as NCBI and Microbesonline. . If a transcription factor is included in the manually curated RegPrecise database, further information from RegPrecise is shown, allowing users to perform comparative analysis.

Expression Profiles

Expression profiles is a plot of the expression ratios (log10) of the module's genes, over all subset of the conditions included in the module. The X-axis represent conditions and the Y-axis represents log10 expression ratios. Each gene is plotted as line plot with different colors. Colored legend for the lines are presented under the plot. This plot is dynamic. Clicking on the gene names in the legend will show/hide the plot for that particular gene. A tooltip will show expression ratio information if you mouseover the lines in the plot.

Motif Locations

Location of the Identified motifs for the module in the upstream regions of the member genes are shown under the expression profiles plot. This plot shows the diagram of the upstream positions of the motifs, colored red and green for motifs #1, and 2, respectively. Intensity of the color is proportional to the significance of the occurence of that motif at a given location. Motifs on the forward and reverse strand are represented over and under the line respectively.

Network

A network view of the module is created using cytoscapeWeb and enables dynamic, interactive exploration of the module properties. In this view, module member genes, motifs, and regulatory influences are represented as peripheral nodes connected to core module node via edges. Module members are green circles, regulators are red triangles and motifs are blue diamonds. Selection of a node gives access to detailed information in a pop-up window, which allows dragging and pinning to compare multiple selections. Selecting module members will show information about the selected gene such as name, species and fucntions. Motif selection will show motif logo image and e-values. Bicluster selction will show expression profile and summary statistics for the module.

GeneModule member RegulatorRegulator MotifMotif

Regulators

For each module, single or AND logic connected regulatory influences are listed under the regulators tab. These regulatory influences are identified by Inferelator. Table shows name of the regulator and its type. tf: Transcription factor, ef: Environmental factor and combiner:Combinatorial influence of a tf or an ef through logic gate. Tabel is sortable by clicking on the arrows next to column headers.

Motifs

Transcription factor binding motifs help to elucidate regulatory mechanism. cMonkey integrates powerful de novo motif detection to identify conditionally co-regulated sets of genes. De novo predicted motifs for each module are listed in the module page as motif logo images along with associated prediction statistics (e-values). The main module page also shows the location of these motifs within the upstream sequences of the module member genes.

Motifs of interest can be broadcasted to RegPredict (currently only available for Desulfovibrio vulgaris Hildenborough) in order to compare conservation in similar species. This integrated motif prediction and comparative analysis provides an additional checkpoint for regulatory motif prediction confidence.

Functions

Biological networks contain sets of regulatory units called functional modules that together play a role in regulation of specific functional processes. Connections between different modules in the network can help identify regulatory relationships such as hierarchy and epistasis. In addition, associating functions with modules enables putative assignment of functions to hypothetical genes. It is therefore essential to identify functional enrichment of modules within the regulatory network.

Functional annotations from single sources are often either not available or not complete. Therefore, we integrated KEGG pathway, Gene Ontology, TIGRFam and COG information as references for functional enrichment analysis.

We use hypergeometric p-values to identify significant overlaps between co-regulated module members and genes assigned to a particular functional annotation category. P-values are corrected for multiple comparisons by using Benjamini-Hochberg correction and filtered for p-values ≤ 0.05.

Network Portal presents functional ontologies from KEGG, GO, TIGRFAM, and COG as separate tables that include function name, type, corrected and uncorrected hypergeometric p-values, and the number of genes assigned to this category out of total number of genes in the module.

Genes

Gene member table shows all the genes included in the module. Listed attributes are;

  1. Name: Gene name or Locus tag
  2. Common Name: Gene short name
  3. Type: Type of the feature, usually CDS.
  4. Gene ID: Link to NCBI Gene ID
  5. Chromosome: Chromosome name from annotation file
  6. Start/End:Feature start and end coordinates
  7. Strand: strand of the gene
  8. Description: Description of the gene from annotation file
  9. TF: If the gene is a Transcription Factor or not.

If you are browsing the Network Portal by using Gaggle/Firegoose, firegoose plugin will capture the NameList of the gene members. Captured names can be saved into your Workspace by clicking on "Capture" in the firegoose toolbar or can be directly sent other desktop and web resources by using "Broadcast" option.

Social

You can start a conversation about this module or join the existing discussion by adding your comments. In order to be able to add your comments you need to sign in by using any of the following services;Disqus, Google, Facebook or Twitter. For full compatibility with other network portal features, we recommend using your Google ID.

Definitions

Residual: is a measure of bicluster quality. Mean bicluster residual is smaller when the expression profile of the genes in the module is "tighter". So smaller residuals are usually indicative of better bicluster quality.

Expression Profile: is a preview of the expression profiles of all the genes under subset of conditions included in the module. Tighter expression profiles are usually indicative of better bicluster quality.

Motif e-value: cMonkey tries to identify two motifs per modules in the upstream sequences of the module member genes. Motif e-value is an indicative of the motif co-occurences between the members of the module.Smaller e-values are indicative of significant sequence motifs. Our experience showed that e-values smaller than 10 are generally indicative of significant motifs.

Genes: Number of genes included in the module.

Functions: We identify functional enrichment of each module by camparing to different functional categories such as KEGG, COG, GO etc. by using hypergeometric function. If the module is significantly enriched for any of the functions, this column will list few of the these functions as an overview. Full list of functions is available upon visiting the module page under the Functions tab.