Organism : Bacillus subtilis | Module List:
Module 97 Profile

GeneModule member RegulatorRegulator MotifMotif
Cytoscape Web
Network Help

A network view of the module is created using cytoscapeWeb and enables dynamic, interactive exploration of the module properties. In this view, module member genes, motifs, and regulatory influences are represented as peripheral nodes connected to core module node via edges.

Module members are green circles, regulators are red triangles and motifs are blue diamonds. Selection of a node gives access to detailed information in a pop-up window, which allows dragging and pinning to compare multiple selections. Selecting module members will show information about the selected gene such as name, species and fucntions. Motif selection will show motif logo image and e-values. Bicluster selction will show expression profile and summary statistics for the module.

GeneModule member RegulatorRegulator MotifMotif
Regulators for Module 97

There are 10 regulatory influences for Module 97

Regulator Table (10)
Regulator Name Type
BSU00800 tf
BSU35520 tf
BSU32870 tf
BSU15690 tf
BSU39850 tf
BSU29110 tf
BSU08990 tf
BSU28410 tf
BSU40990 tf
BSU00470 tf

Regulator Help

For each module, single or AND logic connected regulatory influences are listed under the regulators tab. These regulatory influences are identified by Inferelator. Table shows name of the regulator and its type.

tf: Transcription factor

ef: Environmental factor

combiner: Combinatorial influence of a tf or an ef through logic gate. Table is sortable by clicking on the arrows next to column headers.

Motif information (de novo identified motifs for modules)

There are 2 motifs predicted.

Motif Table (2)
Motif Id e-value Consensus Motif Logo
5148 1.80e+01 AaAAAGAg.agatgg
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5149 2.30e+02 AAagacgcgatTtTcTAaaAaAT
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Motif Help

Transcription factor binding motifs help to elucidate regulatory mechanism. cMonkey integrates powerful de novo motif detection to identify conditionally co-regulated sets of genes. De novo predicted motifs for each module are listed in the module page as motif logo images along with associated prediction statistics (e-values). The main module page also shows the location of these motifs within the upstream sequences of the module member genes.

Motifs of interest can be broadcasted to RegPredict (currently only available for Desulfovibrio vulgaris Hildenborough) in order to compare conservation in similar species. This integrated motif prediction and comparative analysis provides an additional checkpoint for regulatory motif prediction confidence.

Motif e-value: cMonkey tries to identify two motifs per modules in the upstream sequences of the module member genes. Motif e-value is an indicative of the motif co-occurences between the members of the module.Smaller e-values are indicative of significant sequence motifs. Our experience showed that e-values smaller than 10 are generally indicative of significant motifs.

Functional Enrichment

Regulon 97 is enriched for following functions.

KEGG Enrichment Table

Function Name Function Type Unadjusted pvalue Benjamini Hochberg pvalue Genes with function Method
Metabolism kegg category 8.90e-05 8.89e-04 12/25
Metabolism of Cofactors and Vitamins kegg subcategory 0.00e+00 0.00e+00 10/25
Riboflavin metabolism kegg pathway 0.00e+00 0.00e+00 4/25
Folate biosynthesis kegg pathway 0.00e+00 0.00e+00 6/25
Global kegg category 1.92e-03 8.11e-03 9/25
Metabolism kegg subcategory 1.92e-03 8.11e-03 9/25
Metabolic pathways kegg pathway 1.14e-03 5.77e-03 9/25

TIGRFam Enrichment Table

Function Name Function Type Unadjusted pvalue Benjamini& Hochberg pvalue Genes with function Method
DNA metabolism tigr mainrole 6.10e-05 1.28e-04 4/25
DNA replication, recombination, and repair tigr sub1role 3.70e-05 8.50e-05 4/25
Unknown function tigr mainrole 2.04e-03 3.16e-03 3/25
General tigr sub1role 2.00e-04 3.80e-04 3/25
Biosynthesis of cofactors, prosthetic groups, and carriers tigr mainrole 1.00e-06 2.00e-06 6/25
Folic acid tigr sub1role 0.00e+00 0.00e+00 3/25
Riboflavin, FMN, and FAD tigr sub1role 0.00e+00 0.00e+00 3/25

COG Enrichment Table

Function Name Function Type Unadjusted pvalue Benjamini& Hochberg pvalue Genes with function Method
Information storage and processing cog category 9.52e-04 1.75e-03 9/25
Translation, ribosomal structure and biogenesis cog subcategory 1.55e-02 2.44e-02 3/25
Replication, recombination and repair cog subcategory 9.84e-04 1.80e-03 4/25
Coenzyme transport and metabolism cog subcategory 0.00e+00 0.00e+00 10/25
Functions Help

Biological networks contain sets of regulatory units called functional modules that together play a role in regulation of specific functional processes. Connections between different modules in the network can help identify regulatory relationships such as hierarchy and epistasis. In addition, associating functions with modules enables putative assignment of functions to hypothetical genes. It is therefore essential to identify functional enrichment of modules within the regulatory network.

Functional annotations from single sources are often either not available or not complete. Therefore, we integrated KEGG pathway, Gene Ontology, TIGRFam and COG information as references for functional enrichment analysis.

We use hypergeometric p-values to identify significant overlaps between co-regulated module members and genes assigned to a particular functional annotation category. P-values are corrected for multiple comparisons by using Benjamini-Hochberg correction and filtered for p-values ≤ 0.05.

Network Portal presents functional ontologies from KEGG, GO, TIGRFAM, and COG as separate tables that include function name, type, corrected and uncorrected hypergeometric p-values, and the number of genes assigned to this category out of total number of genes in the module.

Members for Module 97

There are 25 genes in Module 97

Gene Member Table (25)
Name Common name Type Gene ID Chromosome Start End Strand Description TF
BSU00010 dnaA CDS None chromosome 410 1750 + chromosomal replication initiation protein (RefSeq) False
BSU00190 dnaX CDS None chromosome 26812 28503 + DNA polymerase III subunits gamma and tau (RefSeq) False
BSU00740 pabB CDS None chromosome 82861 84273 + 4-amino-4-deoxychorismate synthase (para-aminobenzoate synthase) (RefSeq) False
BSU00750 pabA CDS None chromosome 84287 84871 + para-aminobenzoate/anthranilate synthase glutamine amidotransferase component II (RefSeq) False
BSU00760 pabC CDS None chromosome 84871 85752 + 4-amino-4-deoxychorismate lyase (RefSeq) False
BSU00770 sul CDS None chromosome 85734 86591 + dihydropteroate synthase (RefSeq) False
BSU00780 folB CDS None chromosome 86584 86946 + dihydroneopterin aldolase (RefSeq) False
BSU00790 folK CDS None chromosome 86943 87446 + 7,8-dihydro-6-hydroxymethylpterin pyrophosphokinase (RefSeq) False
BSU00800 yazB CDS None chromosome 87398 87607 + putative transcriptional regulator (RefSeq) True
BSU00810 dusB CDS None chromosome 87631 88632 + tRNA-dihydrouridine synthase B (RefSeq) False
BSU00820 lysS CDS None chromosome 88724 90223 + lysyl-tRNA synthetase (RefSeq) False
BSU04260 topB CDS None chromosome 476115 478298 + DNA topoisomerase III (RefSeq) False
BSU23250 ribH CDS None chromosome 2427086 2427550 - 6,7-dimethyl-8-ribityllumazine synthase (RefSeq) False
BSU23260 ribA CDS None chromosome 2427583 2428779 - bifunctional 3,4-dihydroxy-2-butanone 4-phosphate synthase/GTP cyclohydrolase II protein (RefSeq) False
BSU23270 ribE CDS None chromosome 2428794 2429441 - riboflavin synthase subunit alpha (RefSeq) False
BSU23280 ribD CDS None chromosome 2429452 2430537 - fused diaminohydroxyphosphoribosylaminopyrimidine deaminase; 5-amino-6-(5-phosphoribosylamino) uracil reductase (RefSeq) False
BSU24840 yqgS CDS None chromosome 2567806 2569722 - putative anion transporter and exported enzyme (RefSeq) False
BSU25280 recO CDS None chromosome 2608179 2608946 - DNA repair protein RecO (RefSeq) False
BSU25290 era CDS None chromosome 2609274 2610179 - GTP-binding protein Era (RefSeq) False
BSU25300 cdd CDS None chromosome 2610160 2610570 - cytidine/deoxycytidine deaminase (RefSeq) False
BSU32870 yusO CDS None chromosome 3373519 3373986 + putative transcriptional regulator (MarR family) (RefSeq) True
BSU35290 prfB CDS None chromosome 3626169 3627270 - peptide chain release factor 2 (NCBI) False
BSU35500 degS CDS None chromosome 3644409 3645566 - two-component sensor histidine kinase (RefSeq) False
BSU35510 yvyE CDS None chromosome 3645783 3646436 + hypothetical protein (RefSeq) False
BSU35520 yvhJ CDS None chromosome 3646436 3647611 + putative membrane bound transcriptional regulator (RefSeq) True

Genes Help

Gene member table shows all the genes included in the module. Listed attributes are;

  1. Name: Gene name or Locus tag
  2. Common Name: Gene short name
  3. Type: Type of the feature, usually CDS.
  4. Gene ID: Link to NCBI Gene ID
  5. Chromosome: Chromosome name from annotation file
  6. Start/End:Feature start and end coordinates
  7. Strand: strand of the gene
  8. Description: Description of the gene from annotation file
  9. TF: If the gene is a Transcription Factor or not.

If you are browsing the Network Portal by using Gaggle/Firegoose, firegoose plugin will capture the NameList of the gene members. Captured names can be saved into your Workspace by clicking on "Capture" in the firegoose toolbar or can be directly sent other desktop and web resources by using "Broadcast" option.

Comments for Module 97

Please add your comments for this module by using the form below. Your comments will be publicly available.

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Social Help

You can start a conversation about this module or join the existing discussion by adding your comments. In order to be able to add your comments you need to sign in by using any of the following services;Disqus, Google, Facebook or Twitter. For full compatibility with other network portal features, we recommend using your Google ID.

Help

What is a module?

Regulatory units (modules) in the Network Portal are based on the network inference algorithm used. For the current version, modules are based on cMonkey modules and Inferelator regulatory influences on these modules. More specifically, module refers to set of genes that are conditionally co-regulated under subset of the conditions. Identification of modules integrates co-expression, de-novo motif identification, and other functional associations such as operon information and protein-protein interactions.

Module Overview

The landing module page shows quick summary info including co-expression profiles, de-novo identified motifs, and transcription factors and/or environmental factors as regulatory influences. It also includes module residual, motif e-values, conditions and links to other resources such as NCBI and Microbesonline. . If a transcription factor is included in the manually curated RegPrecise database, further information from RegPrecise is shown, allowing users to perform comparative analysis.

Expression Profiles

Expression profiles is a plot of the expression ratios (log10) of the module's genes, over all subset of the conditions included in the module. The X-axis represent conditions and the Y-axis represents log10 expression ratios. Each gene is plotted as line plot with different colors. Colored legend for the lines are presented under the plot. This plot is dynamic. Clicking on the gene names in the legend will show/hide the plot for that particular gene. A tooltip will show expression ratio information if you mouseover the lines in the plot.

Motif Locations

Location of the Identified motifs for the module in the upstream regions of the member genes are shown under the expression profiles plot. This plot shows the diagram of the upstream positions of the motifs, colored red and green for motifs #1, and 2, respectively. Intensity of the color is proportional to the significance of the occurence of that motif at a given location. Motifs on the forward and reverse strand are represented over and under the line respectively.

Network

A network view of the module is created using cytoscapeWeb and enables dynamic, interactive exploration of the module properties. In this view, module member genes, motifs, and regulatory influences are represented as peripheral nodes connected to core module node via edges. Module members are green circles, regulators are red triangles and motifs are blue diamonds. Selection of a node gives access to detailed information in a pop-up window, which allows dragging and pinning to compare multiple selections. Selecting module members will show information about the selected gene such as name, species and fucntions. Motif selection will show motif logo image and e-values. Bicluster selction will show expression profile and summary statistics for the module.

GeneModule member RegulatorRegulator MotifMotif

Regulators

For each module, single or AND logic connected regulatory influences are listed under the regulators tab. These regulatory influences are identified by Inferelator. Table shows name of the regulator and its type. tf: Transcription factor, ef: Environmental factor and combiner:Combinatorial influence of a tf or an ef through logic gate. Tabel is sortable by clicking on the arrows next to column headers.

Motifs

Transcription factor binding motifs help to elucidate regulatory mechanism. cMonkey integrates powerful de novo motif detection to identify conditionally co-regulated sets of genes. De novo predicted motifs for each module are listed in the module page as motif logo images along with associated prediction statistics (e-values). The main module page also shows the location of these motifs within the upstream sequences of the module member genes.

Motifs of interest can be broadcasted to RegPredict (currently only available for Desulfovibrio vulgaris Hildenborough) in order to compare conservation in similar species. This integrated motif prediction and comparative analysis provides an additional checkpoint for regulatory motif prediction confidence.

Functions

Biological networks contain sets of regulatory units called functional modules that together play a role in regulation of specific functional processes. Connections between different modules in the network can help identify regulatory relationships such as hierarchy and epistasis. In addition, associating functions with modules enables putative assignment of functions to hypothetical genes. It is therefore essential to identify functional enrichment of modules within the regulatory network.

Functional annotations from single sources are often either not available or not complete. Therefore, we integrated KEGG pathway, Gene Ontology, TIGRFam and COG information as references for functional enrichment analysis.

We use hypergeometric p-values to identify significant overlaps between co-regulated module members and genes assigned to a particular functional annotation category. P-values are corrected for multiple comparisons by using Benjamini-Hochberg correction and filtered for p-values ≤ 0.05.

Network Portal presents functional ontologies from KEGG, GO, TIGRFAM, and COG as separate tables that include function name, type, corrected and uncorrected hypergeometric p-values, and the number of genes assigned to this category out of total number of genes in the module.

Genes

Gene member table shows all the genes included in the module. Listed attributes are;

  1. Name: Gene name or Locus tag
  2. Common Name: Gene short name
  3. Type: Type of the feature, usually CDS.
  4. Gene ID: Link to NCBI Gene ID
  5. Chromosome: Chromosome name from annotation file
  6. Start/End:Feature start and end coordinates
  7. Strand: strand of the gene
  8. Description: Description of the gene from annotation file
  9. TF: If the gene is a Transcription Factor or not.

If you are browsing the Network Portal by using Gaggle/Firegoose, firegoose plugin will capture the NameList of the gene members. Captured names can be saved into your Workspace by clicking on "Capture" in the firegoose toolbar or can be directly sent other desktop and web resources by using "Broadcast" option.

Social

You can start a conversation about this module or join the existing discussion by adding your comments. In order to be able to add your comments you need to sign in by using any of the following services;Disqus, Google, Facebook or Twitter. For full compatibility with other network portal features, we recommend using your Google ID.

Definitions

Residual: is a measure of bicluster quality. Mean bicluster residual is smaller when the expression profile of the genes in the module is "tighter". So smaller residuals are usually indicative of better bicluster quality.

Expression Profile: is a preview of the expression profiles of all the genes under subset of conditions included in the module. Tighter expression profiles are usually indicative of better bicluster quality.

Motif e-value: cMonkey tries to identify two motifs per modules in the upstream sequences of the module member genes. Motif e-value is an indicative of the motif co-occurences between the members of the module.Smaller e-values are indicative of significant sequence motifs. Our experience showed that e-values smaller than 10 are generally indicative of significant motifs.

Genes: Number of genes included in the module.

Functions: We identify functional enrichment of each module by camparing to different functional categories such as KEGG, COG, GO etc. by using hypergeometric function. If the module is significantly enriched for any of the functions, this column will list few of the these functions as an overview. Full list of functions is available upon visiting the module page under the Functions tab.