Organism : Clostridium acetobutylicum | Module List :
CAC0326

ATPase related to the helicase subunit of Holliday junction resolvase (NCBI ptt file)

CircVis
Functional Annotations (8)
Function System
ATPase related to the helicase subunit of the Holliday junction resolvase cog/ cog
DNA-directed DNA polymerase activity go/ molecular_function
ATP binding go/ molecular_function
nucleus go/ cellular_component
DNA repair go/ biological_process
DNA recombination go/ biological_process
four-way junction helicase activity go/ molecular_function
nucleoside-triphosphatase activity go/ molecular_function
GeneModule member RegulatorRegulator MotifMotif

Cytoscape Web
Regulation information for CAC0326
(Mouseover regulator name to see its description)

CAC0326 is regulated by 16 influences and regulates 0 modules.
Regulators for CAC0326 (16)
Regulator Module Operator
CAC0723 89 tf
CAC1578 89 tf
CAC2605 89 tf
CAC2859 89 tf
CAC3345 89 tf
CAC3406 89 tf
CAC3413 89 tf
CAC3438 89 tf
CAC3651 89 tf
CAC0514 24 tf
CAC2236 24 tf
CAC3324 24 tf
CAC3361 24 tf
CAC3370 24 tf
CAC3466 24 tf
CAC3488 24 tf

Warning: CAC0326 Does not regulate any modules!

Motif information (de novo identified motifs for modules)

There are 4 motifs predicted.

Motif Table (4)
Motif Id e-value Consensus Motif Logo
6702 3.60e-01 TgataGGAGg
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6703 4.80e+03 GGCGCT
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6832 8.20e+02 t.gaGGaG
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6833 1.40e+04 cACAcGG
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Motif Help

Transcription factor binding motifs help to elucidate regulatory mechanism. cMonkey integrates powerful de novo motif detection to identify conditionally co-regulated sets of genes. De novo predicted motifs for each module are listed in the module page as motif logo images along with associated prediction statistics (e-values). The main module page also shows the location of these motifs within the upstream sequences of the module member genes.

Motifs of interest can be broadcasted to RegPredict (currently only available for Desulfovibrio vulgaris Hildenborough) in order to compare conservation in similar species. This integrated motif prediction and comparative analysis provides an additional checkpoint for regulatory motif prediction confidence.

Motif e-value: cMonkey tries to identify two motifs per modules in the upstream sequences of the module member genes. Motif e-value is an indicative of the motif co-occurences between the members of the module.Smaller e-values are indicative of significant sequence motifs. Our experience showed that e-values smaller than 10 are generally indicative of significant motifs.

Functional Enrichment for CAC0326

CAC0326 is enriched for 8 functions in 3 categories.
Enrichment Table (8)
Function System
ATPase related to the helicase subunit of the Holliday junction resolvase cog/ cog
DNA-directed DNA polymerase activity go/ molecular_function
ATP binding go/ molecular_function
nucleus go/ cellular_component
DNA repair go/ biological_process
DNA recombination go/ biological_process
four-way junction helicase activity go/ molecular_function
nucleoside-triphosphatase activity go/ molecular_function
Module neighborhood information for CAC0326

CAC0326 has total of 36 gene neighbors in modules 24, 89
Gene neighbors (36)
Gene Common Name Description Module membership
CAC0071 CAC0071 Uncharacterized conserved protein, YTFE E.coli ortholog (NCBI ptt file) 24, 108
CAC0202 CAC0202 Hydrolase of the alpha/beta superfamily (NCBI ptt file) 24, 288
CAC0203 CAC0203 Flavodoxin (NCBI ptt file) 24, 288
CAC0326 CAC0326 ATPase related to the helicase subunit of Holliday junction resolvase (NCBI ptt file) 24, 89
CAC0351 CAC0351 Hypothetical protein (NCBI ptt file) 89, 309
CAC0598 gerKC Spore germination protein, gerKC (NCBI ptt file) 24, 145
CAC0625 CAC0625 Possible periplasmic aspartyl protease (NCBI ptt file) 24, 266
CAC0629 CAC0629 Predicted hydrolase of the HAD family (NCBI ptt file) 89, 157
CAC0741 CAC0741 Methyl-accepting chemotaxis protein (NCBI ptt file) 24, 285
CAC0755 CAC0755 Multimeric flavodoxin WrbA family protein, diverged or disrupted (NCBI ptt file) 89, 309
CAC0805 CAC0805 Methyl-accepting chemotaxis protein (NCBI ptt file) 24, 207
CAC0921 CAC0921 Metal-dependent amidohydrolase (NCBI ptt file) 24, 108
CAC1186 CAC1186 Hypothetical protein (NCBI ptt file) 89, 309
CAC1221 CAC1221 Hypothetical protein (NCBI ptt file) 1, 89
CAC1351 CAC1351 Periplasmic sugar-binding protein (NCBI ptt file) 24, 267
CAC1578 CAC1578 Predicted transcriptional regulator (NCBI ptt file) 74, 89
CAC1597 CAC1597 Zn-finger DNA-binding domain (NCBI ptt file) 74, 89
CAC1598 CAC1598 Hypothetical protein, CF-32 family (NCBI ptt file) 74, 89
CAC2516 CAC2516 Hypothetical protein (NCBI ptt file) 89, 320
CAC2528 CAC2528 Multimeric flavodoxin WrbA fused to cytochrome c-like domain (NCBI ptt file) 7, 24
CAC2592 CAC2592 6-pyruvoyl-tetrahydropterin synthase related domain; conserved membrane protein (NCBI ptt file) 24, 240
CAC2605 CAC2605 Transcriptional regulator (TetR/AcrR family) (NCBI ptt file) 74, 89
CAC2812 CAC2812 Hypothetical protein (NCBI ptt file) 24, 115
CAC2817 CAC2817 Predicted membrane protein (NCBI ptt file) 74, 89
CAC2898 spoIIR Stage II sporulation protein R (NCBI ptt file) 24, 179
CAC2919 CAC2919 Hypothetical protein (NCBI ptt file) 24, 73
CAC3301 gerKB Spore germination protein GerKB, membrane protein (NCBI ptt file) 24, 240
CAC3324 CAC3324 Predicted transcriptional regulator (NCBI ptt file) 24, 227
CAC3329 CAC3329 Putative Mn transporter, NRAMP family (NCBI ptt file) 24, 40
CAC3331 CAC3331 Alanine racemase (NCBI ptt file) 24, 353
CAC3345 CAC3345 Transcriptional regulator, AcrR family (NCBI ptt file) 74, 89
CAC3363 CAC3363 Hypothetical protein (NCBI ptt file) 89, 309
CAC3413 CAC3413 Transcriptional regulator, MarR/EmrR family (NCBI ptt file) 89, 344
CAC3438 CAC3438 Transcriptional regulator, (BlaI/MecI subfamily) (NCBI ptt file) 89, 309
CAC3610 CAC3610 ABC-type MDR transport system, permease component (NCBI ptt file) 24, 150
CAC3651 CAC3651 Transcriptional regulator containing a DNA-binding HTH domain and an aminotransferase domain (MocR family) (NCBI ptt file) 74, 89
Gene Page Help

Network Tab

If the gene is associated with a module(s), its connection to given modules along with other members of that module are shown as network by using CytoscapeWeb. In this view, each green colored circular nodes represent module member genes, purple colored diamonds represent module motifs and red triangles represent regulators. Each node is connected to module (Bicluster) via edges. This representation provides quick overview of all genes, regulators and motifs for modules. It also allows one to see shared genes/motifs/regulators among diferent modules.

Network representation is interactive. You can zoom in/out and move nodes/edges around. Clicking on a node will open up a window to give more details. For genes, Locus tag, organism, genomic coordinates, NCBI gene ID, whether it is transcription factor or not and any associated functional information will be shown. For regulators, number of modules are shown in addition to gene details. For motifs, e-value, consensus sequence and sequence logo will be shown. For modules, expression profile plot, motif information, functional associations and motif locations for each member of the module will be shown.
You can pin information boxes by using button in the box title and open up additional ones on the same screen for comparative analysis.

Regulation Tab

Regulation tab for each gene includes regulatory influences such as environmental factors or transcription factors or their combinations identified by regulatory network inference algorithms.

If the gene is a member of a module, regulators influencing that module are also considered to regulate the gene. Regulators table list total number of regulatory influences, regulators, modules and type of the influence.

You can see description of the regulator inside the tooltip when you mouseover. In certain cases the regulatory influence is predicted to be the result of the combination of two influences. These are indicated as combiner in the column labeled "Operator".

For transcription factors, an additional table next to regulator table will be show. This table show modules that are influenced by the transcription factor.

Motifs Tab

Network inference algorithm uses de novo motif prediction for assigning genes to modules. If there are any motifs identified in the upstream region of a gene, the motif will be shown here. For each motif sequence logo, consensus and e-value will be shown.

Functions Tab

Identification of functional enrichment for the module members is important in associating predicted motifs and regulatory influences with pathways. As described above, the network inference pipeline includes a functional enrichment module by which hypergeometric p-values are used to identify over representation of functional ontology terms among module members.

Network Portal presents functional ontologies from KEGG, GO, TIGRFAM, and COG as separate tables that include function name, type, corrected and uncorrected hypergeometric p-values, and the number of genes assigned to this category out of total number of genes in the module.

Module Members Tab

Identity of gene members in a module may help to identify potential interactions between different functional modules. Therefore, neighbor genes that share the same module(s) with gene under consideration are shown here. For each memebr, gene name, description and modules that contain it are listed.

Help Tab

This help page. More general help can be accessed by clicking help menu in the main navigation bar.

Social Tab

Network Portal is designed to promote collaboration through social interactions. Therefore interested researchers can share information, questions and updates for a particular gene.

Users can use their Disqus, Facebook, Twitter or Google accounts to connect to this page (We recommend Google). Each module and gene page includes comments tab that lists history of the interactions for that gene. You can browse the history, make updates, raise questions and share these activities with social web.

In the next releases of the network portal, we are planning to create personal space for each user where you can share you space that contains all the analysis steps you did along with relevant information.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend
Comments for CAC0326
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Gene Help

Overview

Gene landing pages present genomic, functional, and regulatory information for individual genes. A circular visualization displays connections between the selected gene and genes in the same modules, with as edges drawn between the respective coordinates of the whole genome.

The gene page also lists functional ontology assignments, module membership, and motifs associated with these modules. Genes in the network inherit regulatory influences from the modules to which they belong. Therefore, the regulatory information for each gene is a collection of all regulatory influences on these modules. These are listed as a table that includes influence name, type, and target module. If the gene is a transcription factor, its target modules are also displayed in a table that provides residual values and number of genes.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend