Organism : Clostridium acetobutylicum | Module List :
CAC0915

Endoglucanase A precursor (endo-1,4-beta-glucanase) (cellulase A), secreted; dockerin domain (NCBI ptt file)

CircVis
Functional Annotations (2)
Function System
polysaccharide catabolic process go/ biological_process
calcium ion binding go/ molecular_function
GeneModule member RegulatorRegulator MotifMotif

Cytoscape Web
Regulation information for CAC0915
(Mouseover regulator name to see its description)

CAC0915 is regulated by 19 influences and regulates 0 modules.
Regulators for CAC0915 (19)
Regulator Module Operator
CAC1070 26 tf
CAC1071 26 tf
CAC1770 26 tf
CAC1867 26 tf
CAC2616 26 tf
CAC3198 26 tf
CAC3433 26 tf
CAC3496 26 tf
CAC0201 312 tf
CAC0859 312 tf
CAC0933 312 tf
CAC1032 312 tf
CAC1578 312 tf
CAC2254 312 tf
CAC2939 312 tf
CAC3166 312 tf
CAC3345 312 tf
CAC3466 312 tf
CAC3488 312 tf

Warning: CAC0915 Does not regulate any modules!

Motif information (de novo identified motifs for modules)

There are 4 motifs predicted.

Motif Table (4)
Motif Id e-value Consensus Motif Logo
6706 3.00e-01 aagGAggg
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6707 1.80e+04 cGGGAC
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7276 1.40e-02 aaGGaGG
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7277 2.50e+03 tAaaag.g.GagaAA
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Motif Help

Transcription factor binding motifs help to elucidate regulatory mechanism. cMonkey integrates powerful de novo motif detection to identify conditionally co-regulated sets of genes. De novo predicted motifs for each module are listed in the module page as motif logo images along with associated prediction statistics (e-values). The main module page also shows the location of these motifs within the upstream sequences of the module member genes.

Motifs of interest can be broadcasted to RegPredict (currently only available for Desulfovibrio vulgaris Hildenborough) in order to compare conservation in similar species. This integrated motif prediction and comparative analysis provides an additional checkpoint for regulatory motif prediction confidence.

Motif e-value: cMonkey tries to identify two motifs per modules in the upstream sequences of the module member genes. Motif e-value is an indicative of the motif co-occurences between the members of the module.Smaller e-values are indicative of significant sequence motifs. Our experience showed that e-values smaller than 10 are generally indicative of significant motifs.

Functional Enrichment for CAC0915

CAC0915 is enriched for 2 functions in 2 categories.
Enrichment Table (2)
Function System
polysaccharide catabolic process go/ biological_process
calcium ion binding go/ molecular_function
Module neighborhood information for CAC0915

CAC0915 has total of 60 gene neighbors in modules 26, 312
Gene neighbors (60)
Gene Common Name Description Module membership
CAC0004 recF RecF, ABC family ATPase (NCBI ptt file) 26, 272
CAC0009 CAC0009 Uncharacterized conserved protein, ortholog of YRXA B.subtilis (NCBI ptt file) 62, 312
CAC0010 CAC0010 Uncharacterized conserved protein, possible metal-binding (NCBI ptt file) 283, 312
CAC0043 CAC0043 Hypothetical protein, CF-3 family (NCBI ptt file) 99, 312
CAC0044 CAC0044 Predicted membrane protein (NCBI ptt file) 264, 312
CAC0090 CAC0090 Xylanase/chitin deacetylase, NodB family (NCBI ptt file) 109, 312
CAC0354 CAC0354 Uncharacterized membrane protein (NCBI ptt file) 279, 312
CAC0568 asd Aspartate semialdehyde dehydrogenase (gene asd) (NCBI ptt file) 26, 258
CAC0618 CAC0618 Uncharacterised permease, similar to ABC transporter (permease) (NCBI ptt file) 40, 312
CAC0726 CAC0726 Ribose 5-phosphate isomerase RpiB (NCBI ptt file) 288, 312
CAC0915 CAC0915 Endoglucanase A precursor (endo-1,4-beta-glucanase) (cellulase A), secreted; dockerin domain (NCBI ptt file) 26, 312
CAC1089 CAC1089 Serine kinase/phosphatase HPr (NCBI ptt file) 26, 159
CAC1162 CAC1162 Hypothetical protein, CF-11 family (NCBI ptt file) 26, 328
CAC1166 CAC1166 Hypothetical protein (NCBI ptt file) 26, 328
CAC1228 cisA Site-specific DNA recombinase (NCBI ptt file) 26, 53
CAC1271 CAC1271 ComC competence related protein (NCBI ptt file) 190, 312
CAC1277 CAC1277 Predicted membrane protein (NCBI ptt file) 312, 328
CAC1452 CAC1452 MDR-type permease (NCBI ptt file) 26, 53
CAC1526 CAC1526 Amidase, related to GSP of E.coli (NCBI ptt file) 26, 301
CAC1527 CAC1527 Spore germination protein GerKB (NCBI ptt file) 26, 183
CAC1605 arbA Ketopantoate reductase (NCBI ptt file) 26, 298
CAC1635 CAC1635 Predicted nucleic acid binding protein, containing 2 S1 domains, YITL B.subtilis ortholog (NCBI ptt file) 109, 312
CAC1829 CAC1829 Predicted inactivated Zn-dependent peptidase, PMBA ortholog (NCBI ptt file) 312, 315
CAC1844 CAC1844 Hypothetical protein (NCBI ptt file) 26, 123
CAC1860 CAC1860 Predicted flavodoxin oxidoreductase (NCBI ptt file) 62, 312
CAC1861 CAC1861 Fragment flavodoxin oxidoreductase (NCBI ptt file) 26, 244
CAC1956 CAC1956 Site-specific recombinases, DNA invertase Pin homolog (NCBI ptt file) 102, 312
CAC2033 CAC2033 Hypothetical protein (NCBI ptt file) 99, 312
CAC2037 CAC2037 Hypothetical protein, CF-39 family (NCBI ptt file) 109, 312
CAC2038 CAC2038 Hypothetical protein, CF-23 family (NCBI ptt file) 273, 312
CAC2056 CAC2056 Hypothetical protein (NCBI ptt file) 26, 359
CAC2096 CAC2096 Hypothetical protein (NCBI ptt file) 69, 312
CAC2098 CAC2098 Hypothetical protein (NCBI ptt file) 267, 312
CAC2102 CAC2102 Prepilin peptidase (NCBI ptt file) 54, 312
CAC2114 CAC2114 Predicted pseudouridylate synthase, YLYB B.subtilis ortholog (NCBI ptt file) 26, 270
CAC2189 CAC2189 Carbamoylphosphate synthase large subunit, short form (NCBI ptt file) 26, 119
CAC2224 cheW Chemotaxis protein CheW (NCBI ptt file) 26, 339
CAC2251 CAC2251 Uncharacterized conserved membrane protein, affecting LPS biosynthesis (NCBI ptt file) 123, 312
CAC2284 ruvB Holliday junction specific DNA helicase, subunit ruvB (NCBI ptt file) 26, 229
CAC2333 spsI DTDP-glucose pyrophosphorylase (NCBI ptt file) 26, 172
CAC2348 CAC2348 Glycosyltransferase (NCBI ptt file) 13, 312
CAC2431 CAC2431 Alkaline phosphatase superfamily enzyme (NCBI ptt file) 62, 312
CAC2434 CAC2434 Membrane associatehistidine kinase with HAMP domain (NCBI ptt file) 44, 312
CAC2489 CAC2489 Predicted membrane protein (NCBI ptt file) 11, 26
CAC2491 CAC2491 Predicted acetyltransferase (NCBI ptt file) 179, 312
CAC2655 CAC2655 Uncharacterized membrane-associated protein, DedA family (NCBI ptt file) 26, 359
CAC2715 CAC2715 Hypothetical protein (NCBI ptt file) 26, 53
CAC2719 CAC2719 Ethanolamin permease (NCBI ptt file) 111, 312
CAC2743 CAC2743 Predicted permease, YXIO B.subtilis ortholog (NCBI ptt file) 26, 240
CAC2762 CAC2762 Polyferredoxin (NCBI ptt file) 26, 150
CAC2831 CAC2831 Conserved membrane protein, YccA family (NCBI ptt file) 312, 366
CAC2908 CAC2908 Spore coat protein cotS related (NCBI ptt file) 190, 312
CAC2910 CAC2910 Spore coat protein cotS related (NCBI ptt file) 190, 312
CAC3222 gcaD UDP-N-acetylglucosamine pyrophosphorylase (NCBI ptt file) 255, 312
CAC3236 CAC3236 Possible transcriptional regulator from YAEG/LRPR family (NCBI ptt file) 247, 312
CAC3237 msmX Multiple sugar-binding ABC-transporter, MSMX ATP-binding protein (NCBI ptt file) 301, 312
CAC3479 CAC3479 Hypothetical protein (NCBI ptt file) 26, 68
CAC3491 CAC3491 Protein, related to general stress protein 26(GS26) of B.subtilis (pyridoxinephosphate oxidase family) (NCBI ptt file) 312, 341
CAC3554 CAC3554 Siderophore/Surfactin synthetase related protein (NCBI ptt file) 26, 208
CAC3609 CAC3609 ABC-type MDR transport system, permease component (NCBI ptt file) 26, 150
Gene Page Help

Network Tab

If the gene is associated with a module(s), its connection to given modules along with other members of that module are shown as network by using CytoscapeWeb. In this view, each green colored circular nodes represent module member genes, purple colored diamonds represent module motifs and red triangles represent regulators. Each node is connected to module (Bicluster) via edges. This representation provides quick overview of all genes, regulators and motifs for modules. It also allows one to see shared genes/motifs/regulators among diferent modules.

Network representation is interactive. You can zoom in/out and move nodes/edges around. Clicking on a node will open up a window to give more details. For genes, Locus tag, organism, genomic coordinates, NCBI gene ID, whether it is transcription factor or not and any associated functional information will be shown. For regulators, number of modules are shown in addition to gene details. For motifs, e-value, consensus sequence and sequence logo will be shown. For modules, expression profile plot, motif information, functional associations and motif locations for each member of the module will be shown.
You can pin information boxes by using button in the box title and open up additional ones on the same screen for comparative analysis.

Regulation Tab

Regulation tab for each gene includes regulatory influences such as environmental factors or transcription factors or their combinations identified by regulatory network inference algorithms.

If the gene is a member of a module, regulators influencing that module are also considered to regulate the gene. Regulators table list total number of regulatory influences, regulators, modules and type of the influence.

You can see description of the regulator inside the tooltip when you mouseover. In certain cases the regulatory influence is predicted to be the result of the combination of two influences. These are indicated as combiner in the column labeled "Operator".

For transcription factors, an additional table next to regulator table will be show. This table show modules that are influenced by the transcription factor.

Motifs Tab

Network inference algorithm uses de novo motif prediction for assigning genes to modules. If there are any motifs identified in the upstream region of a gene, the motif will be shown here. For each motif sequence logo, consensus and e-value will be shown.

Functions Tab

Identification of functional enrichment for the module members is important in associating predicted motifs and regulatory influences with pathways. As described above, the network inference pipeline includes a functional enrichment module by which hypergeometric p-values are used to identify over representation of functional ontology terms among module members.

Network Portal presents functional ontologies from KEGG, GO, TIGRFAM, and COG as separate tables that include function name, type, corrected and uncorrected hypergeometric p-values, and the number of genes assigned to this category out of total number of genes in the module.

Module Members Tab

Identity of gene members in a module may help to identify potential interactions between different functional modules. Therefore, neighbor genes that share the same module(s) with gene under consideration are shown here. For each memebr, gene name, description and modules that contain it are listed.

Help Tab

This help page. More general help can be accessed by clicking help menu in the main navigation bar.

Social Tab

Network Portal is designed to promote collaboration through social interactions. Therefore interested researchers can share information, questions and updates for a particular gene.

Users can use their Disqus, Facebook, Twitter or Google accounts to connect to this page (We recommend Google). Each module and gene page includes comments tab that lists history of the interactions for that gene. You can browse the history, make updates, raise questions and share these activities with social web.

In the next releases of the network portal, we are planning to create personal space for each user where you can share you space that contains all the analysis steps you did along with relevant information.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend
Comments for CAC0915
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Gene Help

Overview

Gene landing pages present genomic, functional, and regulatory information for individual genes. A circular visualization displays connections between the selected gene and genes in the same modules, with as edges drawn between the respective coordinates of the whole genome.

The gene page also lists functional ontology assignments, module membership, and motifs associated with these modules. Genes in the network inherit regulatory influences from the modules to which they belong. Therefore, the regulatory information for each gene is a collection of all regulatory influences on these modules. These are listed as a table that includes influence name, type, and target module. If the gene is a transcription factor, its target modules are also displayed in a table that provides residual values and number of genes.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend