Organism : Clostridium acetobutylicum | Module List :
CAC2001 iorA

Indolepyruvate ferredoxin oxidoreductase, subunit alpha (NCBI ptt file)

CircVis
Functional Annotations (7)
Function System
Indolepyruvate ferredoxin oxidoreductase, alpha and beta subunits cog/ cog
electron transport go/ biological_process
electron carrier activity go/ molecular_function
thiamine pyrophosphate binding go/ molecular_function
indolepyruvate ferredoxin oxidoreductase activity go/ molecular_function
iron-sulfur cluster binding go/ molecular_function
IOR_alpha tigr/ tigrfam
GeneModule member RegulatorRegulator MotifMotif

Cytoscape Web
Regulation information for CAC2001
(Mouseover regulator name to see its description)

CAC2001 is regulated by 17 influences and regulates 0 modules.
Regulators for CAC2001 iorA (17)
Regulator Module Operator
CAC0032 97 tf
CAC0115 97 tf
CAC0231 97 tf
CAC0360 97 tf
CAC0402 97 tf
CAC0459 97 tf
CAC0768 97 tf
CAC0949 97 tf
CAC1280 97 tf
CAC2053 97 tf
CAC2430 97 tf
CAC2794 97 tf
CAC3409 97 tf
CAC3429 97 tf
CAC3525 97 tf
CAC3603 97 tf
CAC3606 97 tf

Warning: CAC2001 Does not regulate any modules!

Motif information (de novo identified motifs for modules)

There are 2 motifs predicted.

Motif Table (2)
Motif Id e-value Consensus Motif Logo
6984 4.50e+02 GAGGgg.G
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6985 2.60e+03 GGGGaaTCG
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Motif Help

Transcription factor binding motifs help to elucidate regulatory mechanism. cMonkey integrates powerful de novo motif detection to identify conditionally co-regulated sets of genes. De novo predicted motifs for each module are listed in the module page as motif logo images along with associated prediction statistics (e-values). The main module page also shows the location of these motifs within the upstream sequences of the module member genes.

Motifs of interest can be broadcasted to RegPredict (currently only available for Desulfovibrio vulgaris Hildenborough) in order to compare conservation in similar species. This integrated motif prediction and comparative analysis provides an additional checkpoint for regulatory motif prediction confidence.

Motif e-value: cMonkey tries to identify two motifs per modules in the upstream sequences of the module member genes. Motif e-value is an indicative of the motif co-occurences between the members of the module.Smaller e-values are indicative of significant sequence motifs. Our experience showed that e-values smaller than 10 are generally indicative of significant motifs.

Functional Enrichment for CAC2001

CAC2001 is enriched for 7 functions in 3 categories.
Enrichment Table (7)
Function System
Indolepyruvate ferredoxin oxidoreductase, alpha and beta subunits cog/ cog
electron transport go/ biological_process
electron carrier activity go/ molecular_function
thiamine pyrophosphate binding go/ molecular_function
indolepyruvate ferredoxin oxidoreductase activity go/ molecular_function
iron-sulfur cluster binding go/ molecular_function
IOR_alpha tigr/ tigrfam
Module neighborhood information for CAC2001

CAC2001 has total of 30 gene neighbors in modules 97, 166
Gene neighbors (30)
Gene Common Name Description Module membership
CAC1022 CAC1022 Thioesterase II of alpha/beta hydrolase superfamily (NCBI ptt file) 166, 322
CAC1464 CAC1464 UVRA-like protein, probably involved in MDR transport (NCBI ptt file) 4, 166
CAC1763 CAC1763 Predicted endonuclease (NCBI ptt file) 166, 270
CAC1988 CAC1988 Ferrichrome-binding periplasmic protein (NCBI ptt file) 68, 97
CAC1990 CAC1990 ABC-type iron (III) transport system, permease component (NCBI ptt file) 166, 294
CAC1992 moaC Molybdenum cofactor biosynthesis enzyme, MoaC (NCBI ptt file) 68, 166
CAC1993 moaA Molybdenum cofactor biosynthesis enzyme MoaA, Fe-S oxidoreductase (NCBI ptt file) 97, 294
CAC1995 CAC1995 Hypothetical protein (NCBI ptt file) 69, 166
CAC1996 CAC1996 Hypothetical protein (NCBI ptt file) 68, 97
CAC1997 CAC1997 Predicted glycosyltransferase (NCBI ptt file) 68, 97
CAC1998 CAC1998 ABC-type transport system, ATPase component (NCBI ptt file) 68, 97
CAC1999 CAC1999 Uncharacterized protein related to hypothetical protein Cj1507c from Campylobacter jejuni (NCBI ptt file) 97, 294
CAC2001 iorA Indolepyruvate ferredoxin oxidoreductase, subunit alpha (NCBI ptt file) 97, 166
CAC2002 CAC2002 Predicted iron-sulfur flavoprotein (NCBI ptt file) 68, 97
CAC2003 CAC2003 Predicted permease (NCBI ptt file) 97, 166
CAC2005 CAC2005 Siderophore/Surfactin synthetase related protein (NCBI ptt file) 97, 166
CAC2006 CAC2006 Enzyme of siderophore/surfactin biosynthesis (NCBI ptt file) 68, 97
CAC2007 CAC2007 Predicted glycosyltransferase (NCBI ptt file) 97, 209
CAC2008 pksF 3-oxoacyl-(acyl-carrier-protein) synthase (NCBI ptt file) 68, 97
CAC2009 mmgB 3-Hydroxyacyl-CoA dehydrogenase (NCBI ptt file) 97, 166
CAC2010 CAC2010 Predicted Fe-S oxidoreductase (NCBI ptt file) 97, 166
CAC2011 fabH Possible 3-oxoacyl-[acyl-carrier-protein] synthase III (NCBI ptt file) 97, 209
CAC2012 fadB Enoyl-CoA hydratase (NCBI ptt file) 97, 294
CAC2013 CAC2013 Hypothetical protein (NCBI ptt file) 53, 97
CAC2014 CAC2014 Predicted esterase (NCBI ptt file) 97, 166
CAC2015 CAC2015 Hypothetical protein (NCBI ptt file) 53, 97
CAC2016 fadB Enoyl-CoA hydratase (NCBI ptt file) 97, 209
CAC2019 CAC2019 Malonyl CoA-acyl carrier protein transacylase (NCBI ptt file) 166, 238
CAC2288 CAC2288 Acyl-protein synthetase, luxE (NCBI ptt file) 166, 314
CAC3198 greA Transcription elongation factor, greA (NCBI ptt file) 166, 224
Gene Page Help

Network Tab

If the gene is associated with a module(s), its connection to given modules along with other members of that module are shown as network by using CytoscapeWeb. In this view, each green colored circular nodes represent module member genes, purple colored diamonds represent module motifs and red triangles represent regulators. Each node is connected to module (Bicluster) via edges. This representation provides quick overview of all genes, regulators and motifs for modules. It also allows one to see shared genes/motifs/regulators among diferent modules.

Network representation is interactive. You can zoom in/out and move nodes/edges around. Clicking on a node will open up a window to give more details. For genes, Locus tag, organism, genomic coordinates, NCBI gene ID, whether it is transcription factor or not and any associated functional information will be shown. For regulators, number of modules are shown in addition to gene details. For motifs, e-value, consensus sequence and sequence logo will be shown. For modules, expression profile plot, motif information, functional associations and motif locations for each member of the module will be shown.
You can pin information boxes by using button in the box title and open up additional ones on the same screen for comparative analysis.

Regulation Tab

Regulation tab for each gene includes regulatory influences such as environmental factors or transcription factors or their combinations identified by regulatory network inference algorithms.

If the gene is a member of a module, regulators influencing that module are also considered to regulate the gene. Regulators table list total number of regulatory influences, regulators, modules and type of the influence.

You can see description of the regulator inside the tooltip when you mouseover. In certain cases the regulatory influence is predicted to be the result of the combination of two influences. These are indicated as combiner in the column labeled "Operator".

For transcription factors, an additional table next to regulator table will be show. This table show modules that are influenced by the transcription factor.

Motifs Tab

Network inference algorithm uses de novo motif prediction for assigning genes to modules. If there are any motifs identified in the upstream region of a gene, the motif will be shown here. For each motif sequence logo, consensus and e-value will be shown.

Functions Tab

Identification of functional enrichment for the module members is important in associating predicted motifs and regulatory influences with pathways. As described above, the network inference pipeline includes a functional enrichment module by which hypergeometric p-values are used to identify over representation of functional ontology terms among module members.

Network Portal presents functional ontologies from KEGG, GO, TIGRFAM, and COG as separate tables that include function name, type, corrected and uncorrected hypergeometric p-values, and the number of genes assigned to this category out of total number of genes in the module.

Module Members Tab

Identity of gene members in a module may help to identify potential interactions between different functional modules. Therefore, neighbor genes that share the same module(s) with gene under consideration are shown here. For each memebr, gene name, description and modules that contain it are listed.

Help Tab

This help page. More general help can be accessed by clicking help menu in the main navigation bar.

Social Tab

Network Portal is designed to promote collaboration through social interactions. Therefore interested researchers can share information, questions and updates for a particular gene.

Users can use their Disqus, Facebook, Twitter or Google accounts to connect to this page (We recommend Google). Each module and gene page includes comments tab that lists history of the interactions for that gene. You can browse the history, make updates, raise questions and share these activities with social web.

In the next releases of the network portal, we are planning to create personal space for each user where you can share you space that contains all the analysis steps you did along with relevant information.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend
Comments for CAC2001
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Gene Help

Overview

Gene landing pages present genomic, functional, and regulatory information for individual genes. A circular visualization displays connections between the selected gene and genes in the same modules, with as edges drawn between the respective coordinates of the whole genome.

The gene page also lists functional ontology assignments, module membership, and motifs associated with these modules. Genes in the network inherit regulatory influences from the modules to which they belong. Therefore, the regulatory information for each gene is a collection of all regulatory influences on these modules. These are listed as a table that includes influence name, type, and target module. If the gene is a transcription factor, its target modules are also displayed in a table that provides residual values and number of genes.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend