Organism : Campylobacter jejuni | Module List :
Cj0262c

putative methyl-accepting chemotaxis signal transduction protein (NCBI ptt file)

CircVis
Functional Annotations (7)
Function System
Methyl-accepting chemotaxis protein cog/ cog
signal transducer activity go/ molecular_function
chemotaxis go/ biological_process
signal transduction go/ biological_process
membrane go/ cellular_component
Two-component system kegg/ kegg pathway
Bacterial chemotaxis kegg/ kegg pathway
GeneModule member RegulatorRegulator MotifMotif

Cytoscape Web
Regulation information for Cj0262c
(Mouseover regulator name to see its description)

Cj0262c is regulated by 8 influences and regulates 0 modules.
Regulators for Cj0262c (8)
Regulator Module Operator
Cj0287c 40 tf
Cj0368c 40 tf
Cj0400 40 tf
Cj0466 40 tf
Cj0571 40 tf
Cj0757 40 tf
Cj1024c 40 tf
Cj1561 40 tf

Warning: Cj0262c Does not regulate any modules!

Motif information (de novo identified motifs for modules)

There are 4 motifs predicted.

Motif Table (4)
Motif Id e-value Consensus Motif Logo
7462 3.50e+03 TCGCCc
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7463 1.30e+04 CTTcCaaGTGAC
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7694 1.30e+02 CggaaaAaTTAtc.ctTtgtAAg
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7695 1.20e+04 GTGaTcGcCAC
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Motif Help

Transcription factor binding motifs help to elucidate regulatory mechanism. cMonkey integrates powerful de novo motif detection to identify conditionally co-regulated sets of genes. De novo predicted motifs for each module are listed in the module page as motif logo images along with associated prediction statistics (e-values). The main module page also shows the location of these motifs within the upstream sequences of the module member genes.

Motifs of interest can be broadcasted to RegPredict (currently only available for Desulfovibrio vulgaris Hildenborough) in order to compare conservation in similar species. This integrated motif prediction and comparative analysis provides an additional checkpoint for regulatory motif prediction confidence.

Motif e-value: cMonkey tries to identify two motifs per modules in the upstream sequences of the module member genes. Motif e-value is an indicative of the motif co-occurences between the members of the module.Smaller e-values are indicative of significant sequence motifs. Our experience showed that e-values smaller than 10 are generally indicative of significant motifs.

Functional Enrichment for Cj0262c

Cj0262c is enriched for 7 functions in 3 categories.
Enrichment Table (7)
Function System
Methyl-accepting chemotaxis protein cog/ cog
signal transducer activity go/ molecular_function
chemotaxis go/ biological_process
signal transduction go/ biological_process
membrane go/ cellular_component
Two-component system kegg/ kegg pathway
Bacterial chemotaxis kegg/ kegg pathway
Module neighborhood information for Cj0262c

Cj0262c has total of 29 gene neighbors in modules 40, 156
Gene neighbors (29)
Gene Common Name Description Module membership
Cj0140 Cj0140 hypothetical protein Cj0140 (NCBI ptt file) 40, 101
Cj0145 Cj0145 hypothetical protein Cj0145 (NCBI ptt file) 40, 131
Cj0173c Cj0173c putative iron-uptake ABC transport system ATP-binding protein (NCBI ptt file) 40, 143
Cj0177 Cj0177 putative lipoprotein (NCBI ptt file) 7, 40
Cj0204 Cj0204 putative integral membrane protein (NCBI ptt file) 104, 156
Cj0224 argC N-acetyl-gamma-glutamyl-phosphate reductase (NCBI ptt file) 104, 156
Cj0233c pyrE putative orotate phosphoribosyltransferase (NCBI ptt file) 138, 156
Cj0262c Cj0262c putative methyl-accepting chemotaxis signal transduction protein (NCBI ptt file) 40, 156
Cj0319 fliG flagellar motor switch protein (NCBI ptt file) 62, 156
Cj0343c Cj0343c putative integral membrane protein (NCBI ptt file) 156, 159
Cj0379c Cj0379c hypothetical protein Cj0379c (NCBI ptt file) 40, 107
Cj0416 Cj0416 hypothetical protein Cj0416 (NCBI ptt file) 2, 156
Cj0760 Cj0760 hypothetical protein Cj0760 (NCBI ptt file) 9, 156
Cj0797c Cj0797c hypothetical protein Cj0797c (NCBI ptt file) 48, 156
Cj0817 glnH glutamine-binding periplasmic protein (NCBI ptt file) 42, 156
Cj0915 Cj0915 putative hydrolase (NCBI ptt file) 33, 40
Cj1143 neuA1 acylneuraminate cytidylyltransferase (NCBI ptt file) 40, 101
Cj1235 Cj1235 putative periplasmic protein (NCBI ptt file) 40, 149
Cj1386 Cj1386 ankyrin-repeat containing protein (NCBI ptt file) 2, 40
Cj1496c Cj1496c putative periplasmic protein (NCBI ptt file) 139, 156
Cj1497c Cj1497c hypothetical protein Cj1497c (NCBI ptt file) 139, 156
Cj1498c purA adenylosuccinate synthetase (NCBI ptt file) 113, 156
Cj1500 Cj1500 putative integral membrane protein (NCBI ptt file) 139, 156
Cj1501 Cj1501 hypothetical protein Cj1501 (NCBI ptt file) 139, 156
Cj1510c fdhB putative formate dehydrogenase iron-sulfur subunit (NCBI ptt file) 42, 156
Cj1561 Cj1561 putative transcriptional regulator (NCBI ptt file) 40, 51
Cj1630 tonB2 putative tonB transport protein (NCBI ptt file) 40, 143
Cj1665 Cj1665 possible lipoprotein thiredoxin (NCBI ptt file) 145, 156
Cj1686c topA DNA topoisomerase I (NCBI ptt file) 48, 156
Gene Page Help

Network Tab

If the gene is associated with a module(s), its connection to given modules along with other members of that module are shown as network by using CytoscapeWeb. In this view, each green colored circular nodes represent module member genes, purple colored diamonds represent module motifs and red triangles represent regulators. Each node is connected to module (Bicluster) via edges. This representation provides quick overview of all genes, regulators and motifs for modules. It also allows one to see shared genes/motifs/regulators among diferent modules.

Network representation is interactive. You can zoom in/out and move nodes/edges around. Clicking on a node will open up a window to give more details. For genes, Locus tag, organism, genomic coordinates, NCBI gene ID, whether it is transcription factor or not and any associated functional information will be shown. For regulators, number of modules are shown in addition to gene details. For motifs, e-value, consensus sequence and sequence logo will be shown. For modules, expression profile plot, motif information, functional associations and motif locations for each member of the module will be shown.
You can pin information boxes by using button in the box title and open up additional ones on the same screen for comparative analysis.

Regulation Tab

Regulation tab for each gene includes regulatory influences such as environmental factors or transcription factors or their combinations identified by regulatory network inference algorithms.

If the gene is a member of a module, regulators influencing that module are also considered to regulate the gene. Regulators table list total number of regulatory influences, regulators, modules and type of the influence.

You can see description of the regulator inside the tooltip when you mouseover. In certain cases the regulatory influence is predicted to be the result of the combination of two influences. These are indicated as combiner in the column labeled "Operator".

For transcription factors, an additional table next to regulator table will be show. This table show modules that are influenced by the transcription factor.

Motifs Tab

Network inference algorithm uses de novo motif prediction for assigning genes to modules. If there are any motifs identified in the upstream region of a gene, the motif will be shown here. For each motif sequence logo, consensus and e-value will be shown.

Functions Tab

Identification of functional enrichment for the module members is important in associating predicted motifs and regulatory influences with pathways. As described above, the network inference pipeline includes a functional enrichment module by which hypergeometric p-values are used to identify over representation of functional ontology terms among module members.

Network Portal presents functional ontologies from KEGG, GO, TIGRFAM, and COG as separate tables that include function name, type, corrected and uncorrected hypergeometric p-values, and the number of genes assigned to this category out of total number of genes in the module.

Module Members Tab

Identity of gene members in a module may help to identify potential interactions between different functional modules. Therefore, neighbor genes that share the same module(s) with gene under consideration are shown here. For each memebr, gene name, description and modules that contain it are listed.

Help Tab

This help page. More general help can be accessed by clicking help menu in the main navigation bar.

Social Tab

Network Portal is designed to promote collaboration through social interactions. Therefore interested researchers can share information, questions and updates for a particular gene.

Users can use their Disqus, Facebook, Twitter or Google accounts to connect to this page (We recommend Google). Each module and gene page includes comments tab that lists history of the interactions for that gene. You can browse the history, make updates, raise questions and share these activities with social web.

In the next releases of the network portal, we are planning to create personal space for each user where you can share you space that contains all the analysis steps you did along with relevant information.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend
Comments for Cj0262c
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Gene Help

Overview

Gene landing pages present genomic, functional, and regulatory information for individual genes. A circular visualization displays connections between the selected gene and genes in the same modules, with as edges drawn between the respective coordinates of the whole genome.

The gene page also lists functional ontology assignments, module membership, and motifs associated with these modules. Genes in the network inherit regulatory influences from the modules to which they belong. Therefore, the regulatory information for each gene is a collection of all regulatory influences on these modules. These are listed as a table that includes influence name, type, and target module. If the gene is a transcription factor, its target modules are also displayed in a table that provides residual values and number of genes.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend