Organism : Campylobacter jejuni | Module List :
Cj1042c

putative transcriptional regulatory protein (NCBI ptt file)

CircVis
Functional Annotations (6)
Function System
AraC-type DNA-binding domain-containing proteins cog/ cog
DNA binding go/ molecular_function
sequence-specific DNA binding transcription factor activity go/ molecular_function
intracellular go/ cellular_component
regulation of transcription, DNA-dependent go/ biological_process
sequence-specific DNA binding go/ molecular_function
GeneModule member RegulatorRegulator MotifMotif

Cytoscape Web
Regulation information for Cj1042c
(Mouseover regulator name to see its description)

Cj1042c is regulated by 2 influences and regulates 2 modules.
Regulators for Cj1042c (2)
Regulator Module Operator
Cj0480c 50 tf
Cj0670 50 tf

Warning: Cj1042c Does not regulate any modules!

Motif information (de novo identified motifs for modules)

There are 4 motifs predicted.

Motif Table (4)
Motif Id e-value Consensus Motif Logo
7482 1.30e+03 .gcta.tttcaAa.cCt.tgC
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7483 2.70e+03 GCGG.aTT
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7624 3.70e+02 gcTtTggTttAtgag
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7625 4.70e+02 CCTTTtgAA.tTttTgtAAttTtA
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Motif Help

Transcription factor binding motifs help to elucidate regulatory mechanism. cMonkey integrates powerful de novo motif detection to identify conditionally co-regulated sets of genes. De novo predicted motifs for each module are listed in the module page as motif logo images along with associated prediction statistics (e-values). The main module page also shows the location of these motifs within the upstream sequences of the module member genes.

Motifs of interest can be broadcasted to RegPredict (currently only available for Desulfovibrio vulgaris Hildenborough) in order to compare conservation in similar species. This integrated motif prediction and comparative analysis provides an additional checkpoint for regulatory motif prediction confidence.

Motif e-value: cMonkey tries to identify two motifs per modules in the upstream sequences of the module member genes. Motif e-value is an indicative of the motif co-occurences between the members of the module.Smaller e-values are indicative of significant sequence motifs. Our experience showed that e-values smaller than 10 are generally indicative of significant motifs.

Functional Enrichment for Cj1042c

Cj1042c is enriched for 6 functions in 3 categories.
Enrichment Table (6)
Function System
AraC-type DNA-binding domain-containing proteins cog/ cog
DNA binding go/ molecular_function
sequence-specific DNA binding transcription factor activity go/ molecular_function
intracellular go/ cellular_component
regulation of transcription, DNA-dependent go/ biological_process
sequence-specific DNA binding go/ molecular_function
Module neighborhood information for Cj1042c

Cj1042c has total of 43 gene neighbors in modules 50, 121
Gene neighbors (43)
Gene Common Name Description Module membership
Cj0009 gltD glutamate synthase (NADPH) small subunit (NCBI ptt file) 50, 155
Cj0010c rnhB ribonuclease HII (NCBI ptt file) 22, 50
Cj0026c Cj0026c hypothetical protein Cj0026c (NCBI ptt file) 50, 68
Cj0097 proB putative glutamate 5-kinase (NCBI ptt file) 1, 121
Cj0344 Cj0344 hypothetical protein Cj0344 (NCBI ptt file) 68, 121
Cj0418c Cj0418c hypothetical protein Cj0418c (NCBI ptt file) 32, 50
Cj0480c Cj0480c putative transcriptional regulator (NCBI ptt file) 50, 122
Cj0497 Cj0497 putative lipoprotein (NCBI ptt file) 50, 100
Cj0541 Cj0541 polyprenyl synthetase (NCBI ptt file) 46, 50
Cj0567 Cj0567 hypothetical protein Cj0567 (NCBI ptt file) 50, 152
Cj0568 Cj0568 hypothetical protein Cj0568 (NCBI ptt file) 49, 50
Cj0591c Cj0591c putative lipoprotein (NCBI ptt file) 50, 167
Cj0602c Cj0602c hypothetical protein Cj0602c (NCBI ptt file) 50, 101
Cj0680c uvrB excinuclease ABC subunit B (NCBI ptt file) 50, 53
Cj0699c glnA glutamine synthetase (NCBI ptt file) 49, 121
Cj0717 Cj0717 hypothetical protein Cj0717 (NCBI ptt file) 90, 121
Cj0832c Cj0832c putative integral membrane protein (NCBI ptt file) 53, 121
Cj0892c Cj0892c putative periplasmic protein (NCBI ptt file) 121, 123
Cj0916c Cj0916c hypothetical protein Cj0916c (NCBI ptt file) 38, 50
Cj0984 Cj0984 hypothetical protein Cj0984 (NCBI ptt file) 50, 76
Cj1042c Cj1042c putative transcriptional regulatory protein (NCBI ptt file) 50, 121
Cj1050c Cj1050c putative transferase (NCBI ptt file) 51, 121
Cj1066 rdxA nitroreductase (NCBI ptt file) 72, 121
Cj1145c Cj1145c hypothetical protein (NCBI) 72, 121
Cj1148 waaF ADP-heptose--LPS heptosyltransferase (NCBI ptt file) 50, 72
Cj1192 dctA putative C4-dicarboxylate transport protein (NCBI ptt file) 20, 121
Cj1219c Cj1219c putative periplasmic protein (NCBI ptt file) 50, 67
Cj1221 groEL 60 kD chaperonin (cpn60) (NCBI ptt file) 9, 121
Cj1259 porA major outer membrane protein (NCBI ptt file) 68, 121
Cj1278c Cj1278c ypothetical protein Cj1278c (NCBI ptt file) 121, 157
Cj1298 Cj1298 hypothetical protein Cj1298 (NCBI ptt file) 72, 121
Cj1305c Cj1305c hypothetical protein Cj1305c (617 family) (NCBI ptt file) 39, 121
Cj1307 Cj1307 putative amino acid activating enzyme (NCBI ptt file) 67, 121
Cj1334 Cj1334 hypothetical prootein Cj1334 (1318 family) (NCBI ptt file) 17, 121
Cj1354 ceuD enterochelin uptake ATP-binding protein (NCBI ptt file) 7, 121
Cj1368 Cj1368 hypothetical protein Cj1368 (NCBI ptt file) 68, 121
Cj1377c Cj1377c putative ferredoxin (NCBI ptt file) 50, 155
Cj1402c pgk phosphoglycerate kinase (NCBI ptt file) 110, 121
Cj1534c Cj1534c possible bacterioferritin (NCBI ptt file) 78, 121
Cj1627c Cj1627c hypothetical protein Cj1627c (NCBI ptt file) 113, 121
Cj1631c Cj1631c hypothetical protein Cj1631c (NCBI ptt file) 50, 75
Cj1726c metA putative homoserine O-succinyltransferase (NCBI ptt file) 50, 89
VIMSS46399 VIMSS46399 None 50, 117
Gene Page Help

Network Tab

If the gene is associated with a module(s), its connection to given modules along with other members of that module are shown as network by using CytoscapeWeb. In this view, each green colored circular nodes represent module member genes, purple colored diamonds represent module motifs and red triangles represent regulators. Each node is connected to module (Bicluster) via edges. This representation provides quick overview of all genes, regulators and motifs for modules. It also allows one to see shared genes/motifs/regulators among diferent modules.

Network representation is interactive. You can zoom in/out and move nodes/edges around. Clicking on a node will open up a window to give more details. For genes, Locus tag, organism, genomic coordinates, NCBI gene ID, whether it is transcription factor or not and any associated functional information will be shown. For regulators, number of modules are shown in addition to gene details. For motifs, e-value, consensus sequence and sequence logo will be shown. For modules, expression profile plot, motif information, functional associations and motif locations for each member of the module will be shown.
You can pin information boxes by using button in the box title and open up additional ones on the same screen for comparative analysis.

Regulation Tab

Regulation tab for each gene includes regulatory influences such as environmental factors or transcription factors or their combinations identified by regulatory network inference algorithms.

If the gene is a member of a module, regulators influencing that module are also considered to regulate the gene. Regulators table list total number of regulatory influences, regulators, modules and type of the influence.

You can see description of the regulator inside the tooltip when you mouseover. In certain cases the regulatory influence is predicted to be the result of the combination of two influences. These are indicated as combiner in the column labeled "Operator".

For transcription factors, an additional table next to regulator table will be show. This table show modules that are influenced by the transcription factor.

Motifs Tab

Network inference algorithm uses de novo motif prediction for assigning genes to modules. If there are any motifs identified in the upstream region of a gene, the motif will be shown here. For each motif sequence logo, consensus and e-value will be shown.

Functions Tab

Identification of functional enrichment for the module members is important in associating predicted motifs and regulatory influences with pathways. As described above, the network inference pipeline includes a functional enrichment module by which hypergeometric p-values are used to identify over representation of functional ontology terms among module members.

Network Portal presents functional ontologies from KEGG, GO, TIGRFAM, and COG as separate tables that include function name, type, corrected and uncorrected hypergeometric p-values, and the number of genes assigned to this category out of total number of genes in the module.

Module Members Tab

Identity of gene members in a module may help to identify potential interactions between different functional modules. Therefore, neighbor genes that share the same module(s) with gene under consideration are shown here. For each memebr, gene name, description and modules that contain it are listed.

Help Tab

This help page. More general help can be accessed by clicking help menu in the main navigation bar.

Social Tab

Network Portal is designed to promote collaboration through social interactions. Therefore interested researchers can share information, questions and updates for a particular gene.

Users can use their Disqus, Facebook, Twitter or Google accounts to connect to this page (We recommend Google). Each module and gene page includes comments tab that lists history of the interactions for that gene. You can browse the history, make updates, raise questions and share these activities with social web.

In the next releases of the network portal, we are planning to create personal space for each user where you can share you space that contains all the analysis steps you did along with relevant information.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend
Comments for Cj1042c
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Gene Help

Overview

Gene landing pages present genomic, functional, and regulatory information for individual genes. A circular visualization displays connections between the selected gene and genes in the same modules, with as edges drawn between the respective coordinates of the whole genome.

The gene page also lists functional ontology assignments, module membership, and motifs associated with these modules. Genes in the network inherit regulatory influences from the modules to which they belong. Therefore, the regulatory information for each gene is a collection of all regulatory influences on these modules. These are listed as a table that includes influence name, type, and target module. If the gene is a transcription factor, its target modules are also displayed in a table that provides residual values and number of genes.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend