Organism : Campylobacter jejuni | Module List :
Cj1718c leuB

3-isopropylmalate dehydrogenase (NCBI ptt file)

CircVis
Functional Annotations (8)
Function System
Isocitrate/isopropylmalate dehydrogenase cog/ cog
3-isopropylmalate dehydrogenase activity go/ molecular_function
cytoplasm go/ cellular_component
leucine biosynthetic process go/ biological_process
Valine leucine and isoleucine biosynthesis kegg/ kegg pathway
Metabolic pathways kegg/ kegg pathway
Biosynthesis of secondary metabolites kegg/ kegg pathway
leuB tigr/ tigrfam
GeneModule member RegulatorRegulator MotifMotif

Cytoscape Web
Regulation information for Cj1718c
(Mouseover regulator name to see its description)

Warning: No Regulators were found for Cj1718c!

Warning: Cj1718c Does not regulate any modules!

Motif information (de novo identified motifs for modules)

There are 4 motifs predicted.

Motif Table (4)
Motif Id e-value Consensus Motif Logo
7418 1.00e+03 Ac.CaAacCa.aA.GCc
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7419 5.80e+03 cTcGCcGTTG
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7652 3.30e+03 CTCaCACTC
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7653 5.20e+03 ATGGcG
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Motif Help

Transcription factor binding motifs help to elucidate regulatory mechanism. cMonkey integrates powerful de novo motif detection to identify conditionally co-regulated sets of genes. De novo predicted motifs for each module are listed in the module page as motif logo images along with associated prediction statistics (e-values). The main module page also shows the location of these motifs within the upstream sequences of the module member genes.

Motifs of interest can be broadcasted to RegPredict (currently only available for Desulfovibrio vulgaris Hildenborough) in order to compare conservation in similar species. This integrated motif prediction and comparative analysis provides an additional checkpoint for regulatory motif prediction confidence.

Motif e-value: cMonkey tries to identify two motifs per modules in the upstream sequences of the module member genes. Motif e-value is an indicative of the motif co-occurences between the members of the module.Smaller e-values are indicative of significant sequence motifs. Our experience showed that e-values smaller than 10 are generally indicative of significant motifs.

Functional Enrichment for Cj1718c

Cj1718c is enriched for 8 functions in 3 categories.
Enrichment Table (8)
Function System
Isocitrate/isopropylmalate dehydrogenase cog/ cog
3-isopropylmalate dehydrogenase activity go/ molecular_function
cytoplasm go/ cellular_component
leucine biosynthetic process go/ biological_process
Valine leucine and isoleucine biosynthesis kegg/ kegg pathway
Metabolic pathways kegg/ kegg pathway
Biosynthesis of secondary metabolites kegg/ kegg pathway
leuB tigr/ tigrfam
Module neighborhood information for Cj1718c

Cj1718c has total of 34 gene neighbors in modules 18, 135
Gene neighbors (34)
Gene Common Name Description Module membership
Cj0014c Cj0014c putative integral membrane protein (NCBI ptt file) 39, 135
Cj0086c ung uracil-DNA glycosylase (NCBI ptt file) 18, 47
Cj0089 Cj0089 putative lipoprotein (NCBI ptt file) 18, 65
Cj0093 Cj0093 putative periplasmic protein (NCBI ptt file) 18, 61
Cj0096 Cj0096 putative GTP-binding protein (NCBI ptt file) 18, 96
Cj0241c Cj0241c putative iron-binding protein (NCBI ptt file) 18, 79
Cj0314 lysA diaminopimelate decarboxylase (NCBI ptt file) 18, 61
Cj0372 Cj0372 hypothetical protein Cj0372 (NCBI ptt file) 13, 18
Cj0468 Cj0468 amino-acid ABC transporter integral membrane protein (NCBI ptt file) 117, 135
Cj0469 Cj0469 amino-acid ABC transporter ATP-binding protein (NCBI ptt file) 80, 135
Cj0842 Cj0842 putative lipoprotein (NCBI ptt file) 18, 162
Cj0845c gltX glutamyl-tRNA synthetase (NCBI ptt file) 18, 138
Cj0878 Cj0878 hypothetical protein Cj0878 (NCBI ptt file) 18, 114
Cj0920c Cj0920c putative ABC-type amino-acid transporter permease protein (NCBI ptt file) 98, 135
Cj0921c peb1A probable ABC-type amino-acid transporter periplasmic solute-binding protein (NCBI ptt file) 97, 135
Cj0960c rnpA putative ribonuclease P protein component (NCBI ptt file) 47, 135
Cj0978c Cj0978c putative lipoprotein (NCBI ptt file) 18, 132
Cj1003c Cj1003c putative integral membrane protein (NCBI ptt file) 18, 24
Cj1081c thiE thiamin-phosphate pyrophosphorylase (NCBI ptt file) 18, 109
Cj1089c Cj1089c hypothetical protein Cj1089c (NCBI ptt file) 18, 118
Cj1091c leuS leucyl-tRNA synthetase (NCBI ptt file) 18, 118
Cj1105 smpB small protein B homolog (NCBI ptt file) 18, 138
Cj1199 Cj1199 putative iron/ascorbate-dependent oxidoreductase (NCBI ptt file) 18, 59
Cj1269c amiA putative N-acetylmuramoyl-L-alanine amidase (NCBI ptt file) 18, 41
Cj1271c tyrS tyrosyl-tRNA synthetase (NCBI ptt file) 18, 138
Cj1311 neuA2 acylneuraminate cytidylyltransferase (NCBI ptt file) 18, 88
Cj1323 Cj1323 hypothetical protein Cj1323 (NCBI ptt file) 18, 81
Cj1423c Cj1423c putative sugar-phosphate nucleotidyltransferase (NCBI ptt file) 18, 109
Cj1566c nuoN NADH dehydrogenase I chain N (NCBI ptt file) 18, 166
Cj1641 murE UDP-N-acetylmuramoylalanyl-D-glutamate--2,6-diami ligase (NCBI ptt file) 18, 44
Cj1655c nhaA1 Na(+)/H(+) antiporter (NCBI ptt file) 18, 132
Cj1715 Cj1715 putative acetyltransferase (NCBI ptt file) 1, 18
Cj1718c leuB 3-isopropylmalate dehydrogenase (NCBI ptt file) 18, 135
Cj1731c ruvC crossover junction endodeoxyribonuclease (NCBI ptt file) 135, 154
Gene Page Help

Network Tab

If the gene is associated with a module(s), its connection to given modules along with other members of that module are shown as network by using CytoscapeWeb. In this view, each green colored circular nodes represent module member genes, purple colored diamonds represent module motifs and red triangles represent regulators. Each node is connected to module (Bicluster) via edges. This representation provides quick overview of all genes, regulators and motifs for modules. It also allows one to see shared genes/motifs/regulators among diferent modules.

Network representation is interactive. You can zoom in/out and move nodes/edges around. Clicking on a node will open up a window to give more details. For genes, Locus tag, organism, genomic coordinates, NCBI gene ID, whether it is transcription factor or not and any associated functional information will be shown. For regulators, number of modules are shown in addition to gene details. For motifs, e-value, consensus sequence and sequence logo will be shown. For modules, expression profile plot, motif information, functional associations and motif locations for each member of the module will be shown.
You can pin information boxes by using button in the box title and open up additional ones on the same screen for comparative analysis.

Regulation Tab

Regulation tab for each gene includes regulatory influences such as environmental factors or transcription factors or their combinations identified by regulatory network inference algorithms.

If the gene is a member of a module, regulators influencing that module are also considered to regulate the gene. Regulators table list total number of regulatory influences, regulators, modules and type of the influence.

You can see description of the regulator inside the tooltip when you mouseover. In certain cases the regulatory influence is predicted to be the result of the combination of two influences. These are indicated as combiner in the column labeled "Operator".

For transcription factors, an additional table next to regulator table will be show. This table show modules that are influenced by the transcription factor.

Motifs Tab

Network inference algorithm uses de novo motif prediction for assigning genes to modules. If there are any motifs identified in the upstream region of a gene, the motif will be shown here. For each motif sequence logo, consensus and e-value will be shown.

Functions Tab

Identification of functional enrichment for the module members is important in associating predicted motifs and regulatory influences with pathways. As described above, the network inference pipeline includes a functional enrichment module by which hypergeometric p-values are used to identify over representation of functional ontology terms among module members.

Network Portal presents functional ontologies from KEGG, GO, TIGRFAM, and COG as separate tables that include function name, type, corrected and uncorrected hypergeometric p-values, and the number of genes assigned to this category out of total number of genes in the module.

Module Members Tab

Identity of gene members in a module may help to identify potential interactions between different functional modules. Therefore, neighbor genes that share the same module(s) with gene under consideration are shown here. For each memebr, gene name, description and modules that contain it are listed.

Help Tab

This help page. More general help can be accessed by clicking help menu in the main navigation bar.

Social Tab

Network Portal is designed to promote collaboration through social interactions. Therefore interested researchers can share information, questions and updates for a particular gene.

Users can use their Disqus, Facebook, Twitter or Google accounts to connect to this page (We recommend Google). Each module and gene page includes comments tab that lists history of the interactions for that gene. You can browse the history, make updates, raise questions and share these activities with social web.

In the next releases of the network portal, we are planning to create personal space for each user where you can share you space that contains all the analysis steps you did along with relevant information.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend
Comments for Cj1718c
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Gene Help

Overview

Gene landing pages present genomic, functional, and regulatory information for individual genes. A circular visualization displays connections between the selected gene and genes in the same modules, with as edges drawn between the respective coordinates of the whole genome.

The gene page also lists functional ontology assignments, module membership, and motifs associated with these modules. Genes in the network inherit regulatory influences from the modules to which they belong. Therefore, the regulatory information for each gene is a collection of all regulatory influences on these modules. These are listed as a table that includes influence name, type, and target module. If the gene is a transcription factor, its target modules are also displayed in a table that provides residual values and number of genes.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend