******************************************************************************** MEME - Motif discovery tool ******************************************************************************** MEME version 4.10.0 (Release date: Wed May 21 10:35:36 2014 +1000) For further information on how to interpret these results or to get a copy of the MEME software please access http://meme.nbcr.net. This file may be used as input to the MAST algorithm for searching sequence databases for matches to groups of motifs. MAST is available for interactive use and downloading at http://meme.nbcr.net. ******************************************************************************** ******************************************************************************** REFERENCE ******************************************************************************** If you use this program in your research, please cite: Timothy L. Bailey and Charles Elkan, "Fitting a mixture model by expectation maximization to discover motifs in biopolymers", Proceedings of the Second International Conference on Intelligent Systems for Molecular Biology, pp. 28-36, AAAI Press, Menlo Park, California, 1994. ******************************************************************************** ******************************************************************************** TRAINING SET ******************************************************************************** DATAFILE= motifs/433/433.seqs.fa ALPHABET= ACGT Sequence name Weight Length Sequence name Weight Length ------------- ------ ------ ------------- ------ ------ 31986 1.0000 500 9529 1.0000 500 8741 1.0000 500 13459 1.0000 500 36940 1.0000 500 47212 1.0000 500 14502 1.0000 500 39429 1.0000 500 9828 1.0000 500 10160 1.0000 500 15692 1.0000 500 49392 1.0000 500 16840 1.0000 500 10257 1.0000 500 10670 1.0000 500 11285 1.0000 500 45181 1.0000 500 51810 1.0000 500 12544 1.0000 500 46014 1.0000 500 42507 1.0000 500 39109 1.0000 500 50461 1.0000 500 41489 1.0000 500 45420 1.0000 500 35490 1.0000 500 48132 1.0000 500 ******************************************************************************** ******************************************************************************** COMMAND LINE SUMMARY ******************************************************************************** This information can also be useful in the event you wish to report a problem with the MEME software. command: meme motifs/433/433.seqs.fa -oc motifs/433 -dna -minw 12 -maxw 21 -nmotifs 3 -maxsize 500000 model: mod= zoops nmotifs= 3 evt= inf object function= E-value of product of p-values width: minw= 12 maxw= 21 minic= 0.00 width: wg= 11 ws= 1 endgaps= yes nsites: minsites= 2 maxsites= 27 wnsites= 0.8 theta: prob= 1 spmap= uni spfuzz= 0.5 global: substring= yes branching= no wbranch= no em: prior= dirichlet b= 0.01 maxiter= 50 distance= 1e-05 data: n= 13500 N= 27 strands: + sample: seed= 0 seqfrac= 1 Letter frequencies in dataset: A 0.262 C 0.246 G 0.231 T 0.261 Background letter frequencies (from dataset with add-one prior applied): A 0.262 C 0.246 G 0.231 T 0.261 ******************************************************************************** ******************************************************************************** MOTIF 1 MEME width = 12 sites = 16 llr = 163 E-value = 5.5e+000 ******************************************************************************** -------------------------------------------------------------------------------- Motif 1 Description -------------------------------------------------------------------------------- Simplified A :::81::::943 pos.-specific C 53::41:1::14 probability G 1:a:3:a:a:53 matrix T 47:229:9:1:: bits 2.1 * * * 1.9 * * * 1.7 * * * 1.5 * ***** Relative 1.3 ** ***** Entropy 1.1 *** ***** (14.7 bits) 0.8 *** ***** 0.6 **** ****** 0.4 **** ******* 0.2 ************ 0.0 ------------ Multilevel CTGACTGTGAGC consensus TC G AA sequence G -------------------------------------------------------------------------------- -------------------------------------------------------------------------------- Motif 1 sites sorted by position p-value -------------------------------------------------------------------------------- Sequence name Start P-value Site ------------- ----- --------- ------------ 45420 412 2.10e-07 ACTGCTCTAA TTGACTGTGAGC TAACAGTTAC 12544 31 3.21e-07 ACCGGATCTA TTGACTGTGAGG TATTGACAAA 15692 91 6.53e-07 AACCGGATAC CTGACTGTGAAA TGCAGATTAT 39429 91 2.38e-06 GCACATTAAT CTGAATGTGAGC TACTGAGAAA 51810 9 3.09e-06 CGGCATGA CTGACTGTGACG GTGAATAATT 10160 220 3.54e-06 GCCTGTGATG GTGACTGTGAGA CGGTCACGGA 48132 389 5.67e-06 GAAACAAGCT CTGACTGCGAGG CACAATGACC 10670 281 7.70e-06 CATAAGCTTT TCGATTGTGAAC GGGGCCTTTT 11285 421 1.03e-05 AGTCTAGTGG CTGAGCGTGAGG TTCATGGACA 9529 276 1.03e-05 ATTTCAAAAT CCGAATGTGAAG AAATCCATAC 13459 112 1.24e-05 CTTCACAGTC TTGAGTGCGAGC CCTTGAAAAG 10257 429 2.71e-05 GTACGACGGT TCGACTGTGTAA GACATGCGGC 14502 130 3.49e-05 GATTCCTAGA GTGTTTGTGAGA GGATGACGAA 9828 143 3.66e-05 GTTCCCAGTT TCGAGCGTGAAA TCGGGACAAC 49392 353 4.37e-05 TATTGCTGTT CTGTGTGTGTAC GAGACGGAGC 31986 481 4.85e-05 AACACCAACA CCGTTTGTGACC TTGGAAGT -------------------------------------------------------------------------------- -------------------------------------------------------------------------------- Motif 1 block diagrams -------------------------------------------------------------------------------- SEQUENCE NAME POSITION P-VALUE MOTIF DIAGRAM ------------- ---------------- ------------- 45420 2.1e-07 411_[+1]_77 12544 3.2e-07 30_[+1]_458 15692 6.5e-07 90_[+1]_398 39429 2.4e-06 90_[+1]_398 51810 3.1e-06 8_[+1]_480 10160 3.5e-06 219_[+1]_269 48132 5.7e-06 388_[+1]_100 10670 7.7e-06 280_[+1]_208 11285 1e-05 420_[+1]_68 9529 1e-05 275_[+1]_213 13459 1.2e-05 111_[+1]_377 10257 2.7e-05 428_[+1]_60 14502 3.5e-05 129_[+1]_359 9828 3.7e-05 142_[+1]_346 49392 4.4e-05 352_[+1]_136 31986 4.9e-05 480_[+1]_8 -------------------------------------------------------------------------------- -------------------------------------------------------------------------------- Motif 1 in BLOCKS format -------------------------------------------------------------------------------- BL MOTIF 1 width=12 seqs=16 45420 ( 412) TTGACTGTGAGC 1 12544 ( 31) TTGACTGTGAGG 1 15692 ( 91) CTGACTGTGAAA 1 39429 ( 91) CTGAATGTGAGC 1 51810 ( 9) CTGACTGTGACG 1 10160 ( 220) GTGACTGTGAGA 1 48132 ( 389) CTGACTGCGAGG 1 10670 ( 281) TCGATTGTGAAC 1 11285 ( 421) CTGAGCGTGAGG 1 9529 ( 276) CCGAATGTGAAG 1 13459 ( 112) TTGAGTGCGAGC 1 10257 ( 429) TCGACTGTGTAA 1 14502 ( 130) GTGTTTGTGAGA 1 9828 ( 143) TCGAGCGTGAAA 1 49392 ( 353) CTGTGTGTGTAC 1 31986 ( 481) CCGTTTGTGACC 1 // -------------------------------------------------------------------------------- -------------------------------------------------------------------------------- Motif 1 position-specific scoring matrix -------------------------------------------------------------------------------- log-odds matrix: alength= 4 w= 12 n= 13203 bayes= 10.4245 E= 5.5e+000 -1064 102 -89 52 -1064 34 -1064 140 -1064 -1064 211 -1064 163 -1064 -1064 -48 -107 83 11 -48 -1064 -98 -1064 175 -1064 -1064 211 -1064 -1064 -98 -1064 175 -1064 -1064 211 -1064 174 -1064 -1064 -106 52 -98 111 -1064 26 61 43 -1064 -------------------------------------------------------------------------------- -------------------------------------------------------------------------------- Motif 1 position-specific probability matrix -------------------------------------------------------------------------------- letter-probability matrix: alength= 4 w= 12 nsites= 16 E= 5.5e+000 0.000000 0.500000 0.125000 0.375000 0.000000 0.312500 0.000000 0.687500 0.000000 0.000000 1.000000 0.000000 0.812500 0.000000 0.000000 0.187500 0.125000 0.437500 0.250000 0.187500 0.000000 0.125000 0.000000 0.875000 0.000000 0.000000 1.000000 0.000000 0.000000 0.125000 0.000000 0.875000 0.000000 0.000000 1.000000 0.000000 0.875000 0.000000 0.000000 0.125000 0.375000 0.125000 0.500000 0.000000 0.312500 0.375000 0.312500 0.000000 -------------------------------------------------------------------------------- -------------------------------------------------------------------------------- Motif 1 regular expression -------------------------------------------------------------------------------- [CT][TC]GA[CG]TGTGA[GA][CAG] -------------------------------------------------------------------------------- Time 6.16 secs. ******************************************************************************** ******************************************************************************** MOTIF 2 MEME width = 12 sites = 19 llr = 181 E-value = 3.8e+001 ******************************************************************************** -------------------------------------------------------------------------------- Motif 2 Description -------------------------------------------------------------------------------- Simplified A 7321::2::::: pos.-specific C :3:2:::61::3 probability G 33:2::61:6:: matrix T :285aa3494a7 bits 2.1 1.9 ** * 1.7 ** * * 1.5 ** * * Relative 1.3 * ** * * Entropy 1.1 * * ** **** (13.7 bits) 0.8 * * ** ***** 0.6 * * ******** 0.4 * * ******** 0.2 * ********** 0.0 ------------ Multilevel AATTTTGCTGTT consensus GC G TT T C sequence G T -------------------------------------------------------------------------------- -------------------------------------------------------------------------------- Motif 2 sites sorted by position p-value -------------------------------------------------------------------------------- Sequence name Start P-value Site ------------- ----- --------- ------------ 46014 245 5.06e-07 GCACTTTTGG AATTTTGTTGTT ACGTTGAGTT 14502 11 6.59e-07 CGAAAGAACT ACTTTTGCTTTT CGCTCGTGTA 9828 106 1.32e-06 GAAAATGGGA ACTGTTGCTGTT GTAGTTCGGT 16840 88 2.55e-06 ATCCGGATAT AGTTTTGTTGTC TCCGGGTTGG 36940 275 4.22e-06 ATATAAGTAA GGTTTTGCTGTC GACGGATTGT 47212 302 5.05e-06 CTACCGGTGC AGTGTTGCTGTC AATAAACCAG 35490 290 5.88e-06 GACCAGTGGC ATTGTTGTTGTT TTTGGTCTGC 51810 479 5.88e-06 TTTTGCTCGT GCTTTTTCTGTT GACTGTTACA 49392 341 5.88e-06 CATCCGTACC AGTATTGCTGTT CTGTGTGTGT 39109 404 1.75e-05 CGTGACTTTC ATTTTTTTTGTC TCTTCGTTAA 45181 458 2.12e-05 GCTCCGTAAA AATTTTATTTTT TACCCCTTGC 39429 458 3.30e-05 GCGAACAATC AATATTTTTGTT GGAGTGGCGA 50461 101 3.86e-05 GTTGCAAAGG GGTCTTGCTTTC AAGACGGAAG 13459 405 3.86e-05 CAAGTACTGA AATCTTTTTTTT TCTCAAGATG 8741 19 6.12e-05 CCAAGGCTGT GCATTTACTGTT TCATTGTCAG 10160 479 6.91e-05 ATTCCTATAC AAAGTTACTGTT GATTAGAAGC 10257 229 7.24e-05 AATTCCTTTG GCTTTTGCCTTT ACAGCTAAGG 41489 68 8.01e-05 GACGCGTCGG ATATTTTCTTTC GGTACTCCAT 45420 92 1.25e-04 TTTACATTCA GTTCTTGGTTTT TGAGGATTCG -------------------------------------------------------------------------------- -------------------------------------------------------------------------------- Motif 2 block diagrams -------------------------------------------------------------------------------- SEQUENCE NAME POSITION P-VALUE MOTIF DIAGRAM ------------- ---------------- ------------- 46014 5.1e-07 244_[+2]_244 14502 6.6e-07 10_[+2]_478 9828 1.3e-06 105_[+2]_383 16840 2.6e-06 87_[+2]_401 36940 4.2e-06 274_[+2]_214 47212 5e-06 301_[+2]_187 35490 5.9e-06 289_[+2]_199 51810 5.9e-06 478_[+2]_10 49392 5.9e-06 340_[+2]_148 39109 1.7e-05 403_[+2]_85 45181 2.1e-05 457_[+2]_31 39429 3.3e-05 457_[+2]_31 50461 3.9e-05 100_[+2]_388 13459 3.9e-05 404_[+2]_84 8741 6.1e-05 18_[+2]_470 10160 6.9e-05 478_[+2]_10 10257 7.2e-05 228_[+2]_260 41489 8e-05 67_[+2]_421 45420 0.00013 91_[+2]_397 -------------------------------------------------------------------------------- -------------------------------------------------------------------------------- Motif 2 in BLOCKS format -------------------------------------------------------------------------------- BL MOTIF 2 width=12 seqs=19 46014 ( 245) AATTTTGTTGTT 1 14502 ( 11) ACTTTTGCTTTT 1 9828 ( 106) ACTGTTGCTGTT 1 16840 ( 88) AGTTTTGTTGTC 1 36940 ( 275) GGTTTTGCTGTC 1 47212 ( 302) AGTGTTGCTGTC 1 35490 ( 290) ATTGTTGTTGTT 1 51810 ( 479) GCTTTTTCTGTT 1 49392 ( 341) AGTATTGCTGTT 1 39109 ( 404) ATTTTTTTTGTC 1 45181 ( 458) AATTTTATTTTT 1 39429 ( 458) AATATTTTTGTT 1 50461 ( 101) GGTCTTGCTTTC 1 13459 ( 405) AATCTTTTTTTT 1 8741 ( 19) GCATTTACTGTT 1 10160 ( 479) AAAGTTACTGTT 1 10257 ( 229) GCTTTTGCCTTT 1 41489 ( 68) ATATTTTCTTTC 1 45420 ( 92) GTTCTTGGTTTT 1 // -------------------------------------------------------------------------------- -------------------------------------------------------------------------------- Motif 2 position-specific scoring matrix -------------------------------------------------------------------------------- log-odds matrix: alength= 4 w= 12 n= 13203 bayes= 9.63365 E= 3.8e+001 139 -1089 45 -1089 1 10 19 -31 -73 -1089 -1089 169 -131 -64 -14 101 -1089 -1089 -1089 194 -1089 -1089 -1089 194 -73 -1089 132 1 -1089 123 -213 50 -1089 -222 -1089 186 -1089 -1089 145 50 -1089 -1089 -1089 194 -1089 36 -1089 139 -------------------------------------------------------------------------------- -------------------------------------------------------------------------------- Motif 2 position-specific probability matrix -------------------------------------------------------------------------------- letter-probability matrix: alength= 4 w= 12 nsites= 19 E= 3.8e+001 0.684211 0.000000 0.315789 0.000000 0.263158 0.263158 0.263158 0.210526 0.157895 0.000000 0.000000 0.842105 0.105263 0.157895 0.210526 0.526316 0.000000 0.000000 0.000000 1.000000 0.000000 0.000000 0.000000 1.000000 0.157895 0.000000 0.578947 0.263158 0.000000 0.578947 0.052632 0.368421 0.000000 0.052632 0.000000 0.947368 0.000000 0.000000 0.631579 0.368421 0.000000 0.000000 0.000000 1.000000 0.000000 0.315789 0.000000 0.684211 -------------------------------------------------------------------------------- -------------------------------------------------------------------------------- Motif 2 regular expression -------------------------------------------------------------------------------- [AG][ACGT]T[TG]TT[GT][CT]T[GT]T[TC] -------------------------------------------------------------------------------- Time 11.96 secs. ******************************************************************************** ******************************************************************************** MOTIF 3 MEME width = 12 sites = 13 llr = 140 E-value = 3.0e+002 ******************************************************************************** -------------------------------------------------------------------------------- Motif 3 Description -------------------------------------------------------------------------------- Simplified A a94:27:2911: pos.-specific C :::5::a6:41: probability G :16281:2:18a matrix T :::3:2::15:: bits 2.1 * * 1.9 * * * 1.7 * * * 1.5 ** * * * * Relative 1.3 ** * * * ** Entropy 1.1 *** * * * ** (15.5 bits) 0.8 *** *** * ** 0.6 ********* ** 0.4 ************ 0.2 ************ 0.0 ------------ Multilevel AAGCGACCATGG consensus AT T G C sequence -------------------------------------------------------------------------------- -------------------------------------------------------------------------------- Motif 3 sites sorted by position p-value -------------------------------------------------------------------------------- Sequence name Start P-value Site ------------- ----- --------- ------------ 13459 238 2.16e-07 TAAAATCTGC AAGTGACCATGG CGAGTCGAGA 10160 83 4.24e-07 TCCTCCGTCA AAGCGTCCATGG TATAGTACGT 16840 211 6.31e-07 CATTGTGCCG AAGGGACCATGG CTAAGCAGAG 15692 277 6.31e-07 AATGCTGCCG AAGCGTCCACGG GAAACGAAAA 11285 455 1.12e-06 TGCAGGTTTT AAGCGACAACGG ATCTAGAGGG 9529 184 2.62e-06 GTCGACACCC AAACGACCAAGG TGCTGCATAA 47212 355 5.88e-06 CGTCTAGAGC AAACGACCACCG CCAGTACCGC 14502 102 7.65e-06 GCCGTCTAAA AAGTGTCCAGGG TTTGGCGATT 31986 327 8.89e-06 ACACAGTATG AAAGAACCATGG TCGCATATTT 45420 447 1.37e-05 CCAAACTCCA AAGTGACGTTGG ATTTTGCGTC 41489 137 1.37e-05 GAACAGGGAC AGACGACGACGG ACACGACACC 10670 372 1.91e-05 TGCTCCAAGC AAACGACAATAG GAAAGCATGC 45181 339 3.94e-05 AGACAAAGGA AAGTAGCGACGG ATGACACACG -------------------------------------------------------------------------------- -------------------------------------------------------------------------------- Motif 3 block diagrams -------------------------------------------------------------------------------- SEQUENCE NAME POSITION P-VALUE MOTIF DIAGRAM ------------- ---------------- ------------- 13459 2.2e-07 237_[+3]_251 10160 4.2e-07 82_[+3]_406 16840 6.3e-07 210_[+3]_278 15692 6.3e-07 276_[+3]_212 11285 1.1e-06 454_[+3]_34 9529 2.6e-06 183_[+3]_305 47212 5.9e-06 354_[+3]_134 14502 7.6e-06 101_[+3]_387 31986 8.9e-06 326_[+3]_162 45420 1.4e-05 446_[+3]_42 41489 1.4e-05 136_[+3]_352 10670 1.9e-05 371_[+3]_117 45181 3.9e-05 338_[+3]_150 -------------------------------------------------------------------------------- -------------------------------------------------------------------------------- Motif 3 in BLOCKS format -------------------------------------------------------------------------------- BL MOTIF 3 width=12 seqs=13 13459 ( 238) AAGTGACCATGG 1 10160 ( 83) AAGCGTCCATGG 1 16840 ( 211) AAGGGACCATGG 1 15692 ( 277) AAGCGTCCACGG 1 11285 ( 455) AAGCGACAACGG 1 9529 ( 184) AAACGACCAAGG 1 47212 ( 355) AAACGACCACCG 1 14502 ( 102) AAGTGTCCAGGG 1 31986 ( 327) AAAGAACCATGG 1 45420 ( 447) AAGTGACGTTGG 1 41489 ( 137) AGACGACGACGG 1 10670 ( 372) AAACGACAATAG 1 45181 ( 339) AAGTAGCGACGG 1 // -------------------------------------------------------------------------------- -------------------------------------------------------------------------------- Motif 3 position-specific scoring matrix -------------------------------------------------------------------------------- log-odds matrix: alength= 4 w= 12 n= 13203 bayes= 10.5177 E= 3.0e+002 193 -1035 -1035 -1035 182 -1035 -159 -1035 56 -1035 141 -1035 -1035 113 -59 24 -77 -1035 187 -1035 140 -1035 -159 -18 -1035 202 -1035 -1035 -77 132 0 -1035 182 -1035 -1035 -176 -176 64 -159 82 -176 -168 187 -1035 -1035 -1035 211 -1035 -------------------------------------------------------------------------------- -------------------------------------------------------------------------------- Motif 3 position-specific probability matrix -------------------------------------------------------------------------------- letter-probability matrix: alength= 4 w= 12 nsites= 13 E= 3.0e+002 1.000000 0.000000 0.000000 0.000000 0.923077 0.000000 0.076923 0.000000 0.384615 0.000000 0.615385 0.000000 0.000000 0.538462 0.153846 0.307692 0.153846 0.000000 0.846154 0.000000 0.692308 0.000000 0.076923 0.230769 0.000000 1.000000 0.000000 0.000000 0.153846 0.615385 0.230769 0.000000 0.923077 0.000000 0.000000 0.076923 0.076923 0.384615 0.076923 0.461538 0.076923 0.076923 0.846154 0.000000 0.000000 0.000000 1.000000 0.000000 -------------------------------------------------------------------------------- -------------------------------------------------------------------------------- Motif 3 regular expression -------------------------------------------------------------------------------- AA[GA][CT]G[AT]C[CG]A[TC]GG -------------------------------------------------------------------------------- Time 18.52 secs. ******************************************************************************** ******************************************************************************** SUMMARY OF MOTIFS ******************************************************************************** -------------------------------------------------------------------------------- Combined block diagrams: non-overlapping sites with p-value < 0.0001 -------------------------------------------------------------------------------- SEQUENCE NAME COMBINED P-VALUE MOTIF DIAGRAM ------------- ---------------- ------------- 31986 3.90e-03 326_[+3(8.89e-06)]_142_\ [+1(4.85e-05)]_8 9529 5.18e-04 183_[+3(2.62e-06)]_80_\ [+1(1.03e-05)]_213 8741 1.62e-01 18_[+2(6.12e-05)]_470 13459 2.22e-06 111_[+1(1.24e-05)]_114_\ [+3(2.16e-07)]_155_[+2(3.86e-05)]_84 36940 1.41e-02 274_[+2(4.22e-06)]_214 47212 2.80e-04 247_[+3(2.30e-05)]_42_\ [+2(5.05e-06)]_41_[+3(5.88e-06)]_134 14502 3.57e-06 10_[+2(6.59e-07)]_79_[+3(7.65e-06)]_\ 16_[+1(3.49e-05)]_359 39429 8.38e-04 90_[+1(2.38e-06)]_355_\ [+2(3.30e-05)]_31 9828 2.90e-04 105_[+2(1.32e-06)]_25_\ [+1(3.66e-05)]_346 10160 2.21e-06 82_[+3(4.24e-07)]_125_\ [+1(3.54e-06)]_247_[+2(6.91e-05)]_10 15692 9.36e-06 90_[+1(6.53e-07)]_174_\ [+3(6.31e-07)]_212 49392 3.14e-03 340_[+2(5.88e-06)]_[+1(4.37e-05)]_\ 136 16840 3.61e-05 87_[+2(2.55e-06)]_111_\ [+3(6.31e-07)]_278 10257 6.21e-03 228_[+2(7.24e-05)]_188_\ [+1(2.71e-05)]_60 10670 1.72e-03 280_[+1(7.70e-06)]_79_\ [+3(1.91e-05)]_117 11285 2.08e-04 420_[+1(1.03e-05)]_22_\ [+3(1.12e-06)]_34 45181 5.63e-03 338_[+3(3.94e-05)]_107_\ [+2(2.12e-05)]_31 51810 1.69e-04 8_[+1(3.09e-06)]_458_[+2(5.88e-06)]_\ 10 12544 3.60e-03 30_[+1(3.21e-07)]_458 46014 5.11e-04 5_[+3(9.51e-05)]_227_[+2(5.06e-07)]_\ 244 42507 7.09e-01 500 39109 3.42e-02 403_[+2(1.75e-05)]_85 50461 1.12e-01 100_[+2(3.86e-05)]_388 41489 4.50e-03 67_[+2(8.01e-05)]_57_[+3(1.37e-05)]_\ 352 45420 6.62e-06 411_[+1(2.10e-07)]_23_\ [+3(1.37e-05)]_42 35490 3.74e-02 289_[+2(5.88e-06)]_199 48132 2.75e-02 388_[+1(5.67e-06)]_100 -------------------------------------------------------------------------------- ******************************************************************************** ******************************************************************************** Stopped because nmotifs = 3 reached. ******************************************************************************** CPU: seaotter.hsd1.wa.comcast.net ********************************************************************************