******************************************************************************** MEME - Motif discovery tool ******************************************************************************** MEME version 4.10.0 (Release date: Wed May 21 10:35:36 2014 +1000) For further information on how to interpret these results or to get a copy of the MEME software please access http://meme.nbcr.net. This file may be used as input to the MAST algorithm for searching sequence databases for matches to groups of motifs. MAST is available for interactive use and downloading at http://meme.nbcr.net. ******************************************************************************** ******************************************************************************** REFERENCE ******************************************************************************** If you use this program in your research, please cite: Timothy L. Bailey and Charles Elkan, "Fitting a mixture model by expectation maximization to discover motifs in biopolymers", Proceedings of the Second International Conference on Intelligent Systems for Molecular Biology, pp. 28-36, AAAI Press, Menlo Park, California, 1994. ******************************************************************************** ******************************************************************************** TRAINING SET ******************************************************************************** DATAFILE= motifs/215/215.seqs.fa ALPHABET= ACGT Sequence name Weight Length Sequence name Weight Length ------------- ------ ------ ------------- ------ ------ 10146 1.0000 500 11011 1.0000 500 11154 1.0000 500 1217 1.0000 500 14875 1.0000 500 16675 1.0000 500 23417 1.0000 500 23514 1.0000 500 2353 1.0000 500 24635 1.0000 500 24767 1.0000 500 25199 1.0000 500 25886 1.0000 500 270259 1.0000 500 33353 1.0000 500 36629 1.0000 500 37464 1.0000 500 38054 1.0000 500 38189 1.0000 500 38576 1.0000 500 4034 1.0000 500 4186 1.0000 500 6017 1.0000 500 6238 1.0000 500 7890 1.0000 500 8042 1.0000 500 8773 1.0000 500 ******************************************************************************** ******************************************************************************** COMMAND LINE SUMMARY ******************************************************************************** This information can also be useful in the event you wish to report a problem with the MEME software. command: meme motifs/215/215.seqs.fa -oc motifs/215 -dna -minw 12 -maxw 21 -nmotifs 3 -maxsize 500000 model: mod= zoops nmotifs= 3 evt= inf object function= E-value of product of p-values width: minw= 12 maxw= 21 minic= 0.00 width: wg= 11 ws= 1 endgaps= yes nsites: minsites= 2 maxsites= 27 wnsites= 0.8 theta: prob= 1 spmap= uni spfuzz= 0.5 global: substring= yes branching= no wbranch= no em: prior= dirichlet b= 0.01 maxiter= 50 distance= 1e-05 data: n= 13500 N= 27 strands: + sample: seed= 0 seqfrac= 1 Letter frequencies in dataset: A 0.271 C 0.232 G 0.229 T 0.269 Background letter frequencies (from dataset with add-one prior applied): A 0.271 C 0.232 G 0.229 T 0.269 ******************************************************************************** ******************************************************************************** MOTIF 1 MEME width = 21 sites = 11 llr = 171 E-value = 3.6e-005 ******************************************************************************** -------------------------------------------------------------------------------- Motif 1 Description -------------------------------------------------------------------------------- Simplified A ::1::223:1:1:1:12:::: pos.-specific C 1::4::332:3:21:41::1: probability G :18255434:49:3a::a:19 matrix T 99155422594:85:57:a81 bits 2.1 * * 1.9 * ** 1.7 * * ** * 1.5 ** * * * ** * Relative 1.3 *** * ** * ** * Entropy 1.1 *** * * ** * **** (22.4 bits) 0.9 *** * * ** * ***** 0.6 *** * * ** ******* 0.4 ****** ************* 0.2 ****** ************* 0.0 --------------------- Multilevel TTGTTGGATTGGTTGTTGTTG consensus CGTCCG T G C sequence G C -------------------------------------------------------------------------------- -------------------------------------------------------------------------------- Motif 1 sites sorted by position p-value -------------------------------------------------------------------------------- Sequence name Start P-value Site ------------- ----- --------- --------------------- 38576 345 5.19e-10 CTGCTATCCA TTGCTAGATTGGTTGCTGTTG TGAGTGGCTG 36629 345 5.19e-10 CTGCTATCCA TTGCTAGATTGGTTGCTGTTG TGAGTGGCTG 8042 251 6.04e-10 ATGTGTTTCA TTGTTGTTGTTGTTGTTGTTG CTGCTGCTGC 6017 182 1.04e-09 TGTGCTGTTC TTGTGTTGTTGGTGGCTGTTG TTGGCACGTC 16675 432 1.96e-08 CTCGTGTTGC TTGGTGAGTTTGTTGTTGTTT TAAACTTCGC 6238 313 4.28e-08 CTTTTGGTTT TTACGTACCTTGTTGCTGTTG CTTTTCGGTT 38054 418 1.02e-07 GCTTGAGCCG TGGTGGCAGTGGCGGTAGTTG CCATCGCATA 2353 88 1.02e-07 AGGATGATTG TTTCGGGTGTCATTGTTGTTG CTGCTATGTG 1217 32 1.89e-07 AGTTGTGGCG TTGTTTCCTTCGTCGTCGTCG TCGTCGTTTT 25199 3 3.71e-07 TG CTGTTTGCCATGTAGTTGTTG CCATGGTGTC 8773 246 6.73e-07 TATCGCTCGT TTGGGGCGGTCGCGGAAGTGG CCGTCTGTCT -------------------------------------------------------------------------------- -------------------------------------------------------------------------------- Motif 1 block diagrams -------------------------------------------------------------------------------- SEQUENCE NAME POSITION P-VALUE MOTIF DIAGRAM ------------- ---------------- ------------- 38576 5.2e-10 344_[+1]_135 36629 5.2e-10 344_[+1]_135 8042 6e-10 250_[+1]_229 6017 1e-09 181_[+1]_298 16675 2e-08 431_[+1]_48 6238 4.3e-08 312_[+1]_167 38054 1e-07 417_[+1]_62 2353 1e-07 87_[+1]_392 1217 1.9e-07 31_[+1]_448 25199 3.7e-07 2_[+1]_477 8773 6.7e-07 245_[+1]_234 -------------------------------------------------------------------------------- -------------------------------------------------------------------------------- Motif 1 in BLOCKS format -------------------------------------------------------------------------------- BL MOTIF 1 width=21 seqs=11 38576 ( 345) TTGCTAGATTGGTTGCTGTTG 1 36629 ( 345) TTGCTAGATTGGTTGCTGTTG 1 8042 ( 251) TTGTTGTTGTTGTTGTTGTTG 1 6017 ( 182) TTGTGTTGTTGGTGGCTGTTG 1 16675 ( 432) TTGGTGAGTTTGTTGTTGTTT 1 6238 ( 313) TTACGTACCTTGTTGCTGTTG 1 38054 ( 418) TGGTGGCAGTGGCGGTAGTTG 1 2353 ( 88) TTTCGGGTGTCATTGTTGTTG 1 1217 ( 32) TTGTTTCCTTCGTCGTCGTCG 1 25199 ( 3) CTGTTTGCCATGTAGTTGTTG 1 8773 ( 246) TTGGGGCGGTCGCGGAAGTGG 1 // -------------------------------------------------------------------------------- -------------------------------------------------------------------------------- Motif 1 position-specific scoring matrix -------------------------------------------------------------------------------- log-odds matrix: alength= 4 w= 21 n= 12960 bayes= 10.5565 E= 3.6e-005 -1010 -135 -1010 176 -1010 -1010 -133 176 -157 -1010 184 -156 -1010 65 -33 76 -1010 -1010 99 102 -57 -1010 99 44 -57 23 67 -56 1 23 25 -56 -1010 -35 67 76 -157 -1010 -1010 176 -1010 23 67 44 -157 -1010 199 -1010 -1010 -35 -1010 161 -157 -135 25 102 -1010 -1010 213 -1010 -157 65 -1010 102 -57 -135 -1010 144 -1010 -1010 213 -1010 -1010 -1010 -1010 190 -1010 -135 -133 161 -1010 -1010 199 -156 -------------------------------------------------------------------------------- -------------------------------------------------------------------------------- Motif 1 position-specific probability matrix -------------------------------------------------------------------------------- letter-probability matrix: alength= 4 w= 21 nsites= 11 E= 3.6e-005 0.000000 0.090909 0.000000 0.909091 0.000000 0.000000 0.090909 0.909091 0.090909 0.000000 0.818182 0.090909 0.000000 0.363636 0.181818 0.454545 0.000000 0.000000 0.454545 0.545455 0.181818 0.000000 0.454545 0.363636 0.181818 0.272727 0.363636 0.181818 0.272727 0.272727 0.272727 0.181818 0.000000 0.181818 0.363636 0.454545 0.090909 0.000000 0.000000 0.909091 0.000000 0.272727 0.363636 0.363636 0.090909 0.000000 0.909091 0.000000 0.000000 0.181818 0.000000 0.818182 0.090909 0.090909 0.272727 0.545455 0.000000 0.000000 1.000000 0.000000 0.090909 0.363636 0.000000 0.545455 0.181818 0.090909 0.000000 0.727273 0.000000 0.000000 1.000000 0.000000 0.000000 0.000000 0.000000 1.000000 0.000000 0.090909 0.090909 0.818182 0.000000 0.000000 0.909091 0.090909 -------------------------------------------------------------------------------- -------------------------------------------------------------------------------- Motif 1 regular expression -------------------------------------------------------------------------------- TTG[TC][TG][GT][GC][ACG][TG]T[GTC]GT[TG]G[TC]TGTTG -------------------------------------------------------------------------------- Time 6.39 secs. ******************************************************************************** ******************************************************************************** MOTIF 2 MEME width = 21 sites = 9 llr = 161 E-value = 7.6e-006 ******************************************************************************** -------------------------------------------------------------------------------- Motif 2 Description -------------------------------------------------------------------------------- Simplified A :92:829344::9761a:416 pos.-specific C 9::a:::2:1a4:318:36:3 probability G 118::81164:6::31:7:91 matrix T ::::2::3::::1:::::::: bits 2.1 * * 1.9 * * * 1.7 * * * * * 1.5 ** * * * * * * Relative 1.3 **** ** * * ** * Entropy 1.1 ******* * **** ***** (25.7 bits) 0.9 ******* * **** ***** 0.6 ******* ************* 0.4 ******* ************* 0.2 ******* ************* 0.0 --------------------- Multilevel CAGCAGAAGACGAAACAGCGA consensus A TA TAG C CG CA C sequence C -------------------------------------------------------------------------------- -------------------------------------------------------------------------------- Motif 2 sites sorted by position p-value -------------------------------------------------------------------------------- Sequence name Start P-value Site ------------- ----- --------- --------------------- 33353 297 2.85e-11 ATCGTTTGGA CAGCAGATGGCGAAGCAGAGC GAGGCGGTCA 270259 305 2.85e-11 ATCGTTTGGA CAGCAGATGGCGAAGCAGAGC GAGGCGGTCA 38576 308 3.77e-10 ACGTCCTCTC CAGCTGAAAACCACACAGCGA TTTCTCCTGC 36629 308 3.77e-10 ACGTCCTCTC CAGCTGAAAACCACACAGCGA TTTCTCCTGC 16675 393 7.47e-09 TACTTCCGAT CAGCAGAGAGCGAAGAAGCGG ACGGCAACCT 11011 425 9.29e-09 AACCCTCCCT CAGCAAAAAGCCAAACACAAA CAACAAAGGA 4186 28 1.70e-08 GCAGGGGTGG GAGCAAACGCCGAAACACCGC CCAAGACAGC 37464 150 2.47e-08 AATACTAGCA CAACAGACGACCACCGACCGA CCACTGACCG 38054 378 5.42e-08 TCTTTGGAAC CGACAGGTGACGTAACAGAGA AAGTCGGTAG -------------------------------------------------------------------------------- -------------------------------------------------------------------------------- Motif 2 block diagrams -------------------------------------------------------------------------------- SEQUENCE NAME POSITION P-VALUE MOTIF DIAGRAM ------------- ---------------- ------------- 33353 2.9e-11 296_[+2]_183 270259 2.9e-11 304_[+2]_175 38576 3.8e-10 307_[+2]_172 36629 3.8e-10 307_[+2]_172 16675 7.5e-09 392_[+2]_87 11011 9.3e-09 424_[+2]_55 4186 1.7e-08 27_[+2]_452 37464 2.5e-08 149_[+2]_330 38054 5.4e-08 377_[+2]_102 -------------------------------------------------------------------------------- -------------------------------------------------------------------------------- Motif 2 in BLOCKS format -------------------------------------------------------------------------------- BL MOTIF 2 width=21 seqs=9 33353 ( 297) CAGCAGATGGCGAAGCAGAGC 1 270259 ( 305) CAGCAGATGGCGAAGCAGAGC 1 38576 ( 308) CAGCTGAAAACCACACAGCGA 1 36629 ( 308) CAGCTGAAAACCACACAGCGA 1 16675 ( 393) CAGCAGAGAGCGAAGAAGCGG 1 11011 ( 425) CAGCAAAAAGCCAAACACAAA 1 4186 ( 28) GAGCAAACGCCGAAACACCGC 1 37464 ( 150) CAACAGACGACCACCGACCGA 1 38054 ( 378) CGACAGGTGACGTAACAGAGA 1 // -------------------------------------------------------------------------------- -------------------------------------------------------------------------------- Motif 2 position-specific scoring matrix -------------------------------------------------------------------------------- log-odds matrix: alength= 4 w= 21 n= 12960 bayes= 11.3393 E= 7.6e-006 -982 194 -104 -982 171 -982 -104 -982 -28 -982 176 -982 -982 211 -982 -982 152 -982 -982 -27 -28 -982 176 -982 171 -982 -104 -982 30 -6 -104 31 71 -982 128 -982 71 -106 96 -982 -982 211 -982 -982 -982 94 128 -982 171 -982 -982 -127 130 52 -982 -982 104 -106 54 -982 -128 175 -104 -982 188 -982 -982 -982 -982 52 154 -982 71 126 -982 -982 -128 -982 196 -982 104 52 -104 -982 -------------------------------------------------------------------------------- -------------------------------------------------------------------------------- Motif 2 position-specific probability matrix -------------------------------------------------------------------------------- letter-probability matrix: alength= 4 w= 21 nsites= 9 E= 7.6e-006 0.000000 0.888889 0.111111 0.000000 0.888889 0.000000 0.111111 0.000000 0.222222 0.000000 0.777778 0.000000 0.000000 1.000000 0.000000 0.000000 0.777778 0.000000 0.000000 0.222222 0.222222 0.000000 0.777778 0.000000 0.888889 0.000000 0.111111 0.000000 0.333333 0.222222 0.111111 0.333333 0.444444 0.000000 0.555556 0.000000 0.444444 0.111111 0.444444 0.000000 0.000000 1.000000 0.000000 0.000000 0.000000 0.444444 0.555556 0.000000 0.888889 0.000000 0.000000 0.111111 0.666667 0.333333 0.000000 0.000000 0.555556 0.111111 0.333333 0.000000 0.111111 0.777778 0.111111 0.000000 1.000000 0.000000 0.000000 0.000000 0.000000 0.333333 0.666667 0.000000 0.444444 0.555556 0.000000 0.000000 0.111111 0.000000 0.888889 0.000000 0.555556 0.333333 0.111111 0.000000 -------------------------------------------------------------------------------- -------------------------------------------------------------------------------- Motif 2 regular expression -------------------------------------------------------------------------------- CA[GA]C[AT][GA]A[ATC][GA][AG]C[GC]A[AC][AG]CA[GC][CA]G[AC] -------------------------------------------------------------------------------- Time 13.14 secs. ******************************************************************************** ******************************************************************************** MOTIF 3 MEME width = 20 sites = 12 llr = 179 E-value = 5.7e-005 ******************************************************************************** -------------------------------------------------------------------------------- Motif 3 Description -------------------------------------------------------------------------------- Simplified A :3a1:414:1::2::33:36 pos.-specific C 94:4113418a:36:8758: probability G :3:283:19::81:a::::3 matrix T 1::32371:1:344:::5:1 bits 2.1 * * 1.9 * * * 1.7 * * * * * 1.5 * * * * * Relative 1.3 * * **** ** * Entropy 1.1 * * * **** ****** (21.6 bits) 0.9 * * * * **** ****** 0.6 * * * * **** ******* 0.4 *** * ****** ******* 0.2 ******************** 0.0 -------------------- Multilevel CCACGATAGCCGTCGCCCCA consensus A T GCC TCT AATAG sequence G T -------------------------------------------------------------------------------- -------------------------------------------------------------------------------- Motif 3 sites sorted by position p-value -------------------------------------------------------------------------------- Sequence name Start P-value Site ------------- ----- --------- -------------------- 1217 344 4.76e-10 TCAGCGAAGT CAAGGGTCGCCGTCGCCTCA CCATCCAAGG 38054 56 1.02e-08 CTAACATTTT CGAGGTTAGCCGCTGCATCA ACAGATTCGC 38576 123 2.65e-08 ACGTGCACCT CCATGGCAGCCGTCGACCAG CAGTAACACA 36629 123 2.65e-08 ACGTGCACCT CCATGGCAGCCGTCGACCAG CAGTAACACA 33353 265 3.22e-08 TGTGGTTCTG CCACTTTCGCCTCTGCCTCG CCATCGTTTG 270259 273 3.22e-08 TGTGGTTCTG CCACTTTCGCCTCTGCCTCG CCATCGTTTG 24635 402 4.28e-08 TGGACATGAT CCATGATCGTCGTCGCACAA TCTACAAGCC 8773 10 7.92e-08 CTCTACGTG CAAAGCTTGCCGCCGCCCCA CCGCCAACCA 24767 404 1.01e-07 ATCCGACCAT TGACGACCGCCGACGCATCA AGCAAGCATC 23514 386 1.87e-07 AGCTAAGCCG CAACCATACCCTTCGCCCCA GTACAGAGCC 23417 323 3.48e-07 TGGATGATTC CAATGATGGCCGATGACTCT GCTTGTGGAC 38189 442 6.03e-07 CTTCAACGAA CGACGAAAGACGGTGCACCA CAGTACACAC -------------------------------------------------------------------------------- -------------------------------------------------------------------------------- Motif 3 block diagrams -------------------------------------------------------------------------------- SEQUENCE NAME POSITION P-VALUE MOTIF DIAGRAM ------------- ---------------- ------------- 1217 4.8e-10 343_[+3]_137 38054 1e-08 55_[+3]_425 38576 2.7e-08 122_[+3]_358 36629 2.7e-08 122_[+3]_358 33353 3.2e-08 264_[+3]_216 270259 3.2e-08 272_[+3]_208 24635 4.3e-08 401_[+3]_79 8773 7.9e-08 9_[+3]_471 24767 1e-07 403_[+3]_77 23514 1.9e-07 385_[+3]_95 23417 3.5e-07 322_[+3]_158 38189 6e-07 441_[+3]_39 -------------------------------------------------------------------------------- -------------------------------------------------------------------------------- Motif 3 in BLOCKS format -------------------------------------------------------------------------------- BL MOTIF 3 width=20 seqs=12 1217 ( 344) CAAGGGTCGCCGTCGCCTCA 1 38054 ( 56) CGAGGTTAGCCGCTGCATCA 1 38576 ( 123) CCATGGCAGCCGTCGACCAG 1 36629 ( 123) CCATGGCAGCCGTCGACCAG 1 33353 ( 265) CCACTTTCGCCTCTGCCTCG 1 270259 ( 273) CCACTTTCGCCTCTGCCTCG 1 24635 ( 402) CCATGATCGTCGTCGCACAA 1 8773 ( 10) CAAAGCTTGCCGCCGCCCCA 1 24767 ( 404) TGACGACCGCCGACGCATCA 1 23514 ( 386) CAACCATACCCTTCGCCCCA 1 23417 ( 323) CAATGATGGCCGATGACTCT 1 38189 ( 442) CGACGAAAGACGGTGCACCA 1 // -------------------------------------------------------------------------------- -------------------------------------------------------------------------------- Motif 3 position-specific scoring matrix -------------------------------------------------------------------------------- log-odds matrix: alength= 4 w= 20 n= 12987 bayes= 9.73709 E= 5.7e-005 -1023 198 -1023 -169 30 85 13 -1023 188 -1023 -1023 -1023 -170 85 -46 31 -1023 -147 171 -69 62 -147 13 -10 -170 11 -1023 131 62 85 -146 -169 -1023 -147 200 -1023 -170 185 -1023 -169 -1023 211 -1023 -1023 -1023 -1023 171 -10 -70 52 -146 63 -1023 133 -1023 63 -1023 -1023 213 -1023 -11 169 -1023 -1023 30 152 -1023 -1023 -1023 111 -1023 90 -11 169 -1023 -1023 111 -1023 54 -169 -------------------------------------------------------------------------------- -------------------------------------------------------------------------------- Motif 3 position-specific probability matrix -------------------------------------------------------------------------------- letter-probability matrix: alength= 4 w= 20 nsites= 12 E= 5.7e-005 0.000000 0.916667 0.000000 0.083333 0.333333 0.416667 0.250000 0.000000 1.000000 0.000000 0.000000 0.000000 0.083333 0.416667 0.166667 0.333333 0.000000 0.083333 0.750000 0.166667 0.416667 0.083333 0.250000 0.250000 0.083333 0.250000 0.000000 0.666667 0.416667 0.416667 0.083333 0.083333 0.000000 0.083333 0.916667 0.000000 0.083333 0.833333 0.000000 0.083333 0.000000 1.000000 0.000000 0.000000 0.000000 0.000000 0.750000 0.250000 0.166667 0.333333 0.083333 0.416667 0.000000 0.583333 0.000000 0.416667 0.000000 0.000000 1.000000 0.000000 0.250000 0.750000 0.000000 0.000000 0.333333 0.666667 0.000000 0.000000 0.000000 0.500000 0.000000 0.500000 0.250000 0.750000 0.000000 0.000000 0.583333 0.000000 0.333333 0.083333 -------------------------------------------------------------------------------- -------------------------------------------------------------------------------- Motif 3 regular expression -------------------------------------------------------------------------------- C[CAG]A[CT]G[AGT][TC][AC]GCC[GT][TC][CT]G[CA][CA][CT][CA][AG] -------------------------------------------------------------------------------- Time 19.67 secs. ******************************************************************************** ******************************************************************************** SUMMARY OF MOTIFS ******************************************************************************** -------------------------------------------------------------------------------- Combined block diagrams: non-overlapping sites with p-value < 0.0001 -------------------------------------------------------------------------------- SEQUENCE NAME COMBINED P-VALUE MOTIF DIAGRAM ------------- ---------------- ------------- 10146 2.86e-01 500 11011 1.23e-04 424_[+2(9.29e-09)]_55 11154 7.42e-02 500 1217 4.65e-09 31_[+1(1.89e-07)]_291_\ [+3(4.76e-10)]_137 14875 1.82e-01 500 16675 3.18e-09 392_[+2(7.47e-09)]_18_\ [+1(1.96e-08)]_48 23417 4.22e-04 322_[+3(3.48e-07)]_158 23514 1.43e-03 385_[+3(1.87e-07)]_95 2353 1.55e-03 87_[+1(1.02e-07)]_392 24635 4.26e-04 401_[+3(4.28e-08)]_79 24767 2.78e-04 403_[+3(1.01e-07)]_77 25199 1.18e-04 2_[+1(3.71e-07)]_277_[+3(3.67e-05)]_\ 180 25886 2.17e-03 101_[+2(1.50e-05)]_151_\ [+1(4.28e-05)]_206 270259 3.23e-11 272_[+3(3.22e-08)]_12_\ [+2(2.85e-11)]_175 33353 3.23e-11 264_[+3(3.22e-08)]_12_\ [+2(2.85e-11)]_183 36629 5.32e-16 122_[+3(2.65e-08)]_165_\ [+2(3.77e-10)]_16_[+1(5.19e-10)]_135 37464 1.88e-04 149_[+2(2.47e-08)]_330 38054 3.55e-12 55_[+3(1.02e-08)]_302_\ [+2(5.42e-08)]_19_[+1(1.02e-07)]_62 38189 1.07e-03 441_[+3(6.03e-07)]_39 38576 5.32e-16 122_[+3(2.65e-08)]_165_\ [+2(3.77e-10)]_16_[+1(5.19e-10)]_135 4034 5.38e-01 500 4186 1.58e-04 27_[+2(1.70e-08)]_452 6017 3.83e-05 181_[+1(1.04e-09)]_298 6238 4.61e-05 277_[+1(2.62e-05)]_14_\ [+1(4.28e-08)]_167 7890 2.43e-01 500 8042 8.82e-06 250_[+1(6.04e-10)]_12_\ [+1(5.38e-06)]_196 8773 4.35e-07 9_[+3(7.92e-08)]_216_[+1(6.73e-07)]_\ 234 -------------------------------------------------------------------------------- ******************************************************************************** ******************************************************************************** Stopped because nmotifs = 3 reached. ******************************************************************************** CPU: seaotter.hsd1.wa.comcast.net ********************************************************************************