******************************************************************************** MEME - Motif discovery tool ******************************************************************************** MEME version 4.10.0 (Release date: Wed May 21 10:35:36 2014 +1000) For further information on how to interpret these results or to get a copy of the MEME software please access http://meme.nbcr.net. This file may be used as input to the MAST algorithm for searching sequence databases for matches to groups of motifs. MAST is available for interactive use and downloading at http://meme.nbcr.net. ******************************************************************************** ******************************************************************************** REFERENCE ******************************************************************************** If you use this program in your research, please cite: Timothy L. Bailey and Charles Elkan, "Fitting a mixture model by expectation maximization to discover motifs in biopolymers", Proceedings of the Second International Conference on Intelligent Systems for Molecular Biology, pp. 28-36, AAAI Press, Menlo Park, California, 1994. ******************************************************************************** ******************************************************************************** TRAINING SET ******************************************************************************** DATAFILE= motifs/343/343.seqs.fa ALPHABET= ACGT Sequence name Weight Length Sequence name Weight Length ------------- ------ ------ ------------- ------ ------ 10010 1.0000 500 10509 1.0000 500 10536 1.0000 500 10713 1.0000 500 11540 1.0000 500 2000 1.0000 500 261696 1.0000 500 262191 1.0000 500 263044 1.0000 500 263201 1.0000 500 263252 1.0000 500 263605 1.0000 500 264036 1.0000 500 5669 1.0000 500 6604 1.0000 500 6614 1.0000 500 7301 1.0000 500 7394 1.0000 500 7833 1.0000 500 9241 1.0000 500 9259 1.0000 500 9279 1.0000 500 9306 1.0000 500 9309 1.0000 500 9656 1.0000 500 bd1097 1.0000 500 bd1098 1.0000 500 bd788 1.0000 500 bd810 1.0000 157 bd813 1.0000 500 ******************************************************************************** ******************************************************************************** COMMAND LINE SUMMARY ******************************************************************************** This information can also be useful in the event you wish to report a problem with the MEME software. command: meme motifs/343/343.seqs.fa -oc motifs/343 -dna -minw 12 -maxw 21 -nmotifs 3 -maxsize 500000 model: mod= zoops nmotifs= 3 evt= inf object function= E-value of product of p-values width: minw= 12 maxw= 21 minic= 0.00 width: wg= 11 ws= 1 endgaps= yes nsites: minsites= 2 maxsites= 30 wnsites= 0.8 theta: prob= 1 spmap= uni spfuzz= 0.5 global: substring= yes branching= no wbranch= no em: prior= dirichlet b= 0.01 maxiter= 50 distance= 1e-05 data: n= 14657 N= 30 strands: + sample: seed= 0 seqfrac= 1 Letter frequencies in dataset: A 0.289 C 0.220 G 0.233 T 0.258 Background letter frequencies (from dataset with add-one prior applied): A 0.289 C 0.220 G 0.233 T 0.258 ******************************************************************************** ******************************************************************************** MOTIF 1 MEME width = 21 sites = 8 llr = 173 E-value = 9.7e-016 ******************************************************************************** -------------------------------------------------------------------------------- Motif 1 Description -------------------------------------------------------------------------------- Simplified A ::5::1::13:4:1a:::::: pos.-specific C a:1aa91968a:85:4::a:: probability G :13:::::::::::::5a::6 matrix T :91:::913::634:65::a4 bits 2.2 * ** * ** 2.0 * ** * *** 1.7 * ** * * *** 1.5 ** ***** * * *** Relative 1.3 ** ***** ** * * *** Entropy 1.1 ** ***** ** * ******* (31.2 bits) 0.9 ** ********** ******* 0.7 ** ****************** 0.4 ** ****************** 0.2 ********************* 0.0 --------------------- Multilevel CTACCCTCCCCTCCATGGCTG consensus G TA ATT CT T sequence -------------------------------------------------------------------------------- -------------------------------------------------------------------------------- Motif 1 sites sorted by position p-value -------------------------------------------------------------------------------- Sequence name Start P-value Site ------------- ----- --------- --------------------- 9656 363 1.22e-12 GAACTCAACC CTACCCTCCCCTCTACTGCTG ATTCTAGACA 6614 363 1.22e-12 GAACTCAACC CTACCCTCCCCTCTACTGCTG ATTCTAGACA 11540 363 1.22e-12 GAACTCAACC CTACCCTCCCCTCTACTGCTG ATTCTAGACA bd813 326 4.67e-11 GTCGGTCCAC CTACCCTTTCCTCCATGGCTG CGGCCTCCTC bd788 312 1.82e-10 CACATGAGTG CTGCCCTCCACATCATGGCTT ATTTGCGTGA 263252 444 1.82e-10 CACATGAGTG CTGCCCTCCACATCATGGCTT ATTTGCGTGA 9279 277 4.05e-10 TTTGACAATT CTTCCATCACCTCCATTGCTG ATTGTGCCCA 10010 187 2.15e-09 ACGCAGCAGT CGCCCCCCTCCACAATGGCTT TGGTAATATT -------------------------------------------------------------------------------- -------------------------------------------------------------------------------- Motif 1 block diagrams -------------------------------------------------------------------------------- SEQUENCE NAME POSITION P-VALUE MOTIF DIAGRAM ------------- ---------------- ------------- 9656 1.2e-12 362_[+1]_117 6614 1.2e-12 362_[+1]_117 11540 1.2e-12 362_[+1]_117 bd813 4.7e-11 325_[+1]_154 bd788 1.8e-10 311_[+1]_168 263252 1.8e-10 443_[+1]_36 9279 4e-10 276_[+1]_203 10010 2.1e-09 186_[+1]_293 -------------------------------------------------------------------------------- -------------------------------------------------------------------------------- Motif 1 in BLOCKS format -------------------------------------------------------------------------------- BL MOTIF 1 width=21 seqs=8 9656 ( 363) CTACCCTCCCCTCTACTGCTG 1 6614 ( 363) CTACCCTCCCCTCTACTGCTG 1 11540 ( 363) CTACCCTCCCCTCTACTGCTG 1 bd813 ( 326) CTACCCTTTCCTCCATGGCTG 1 bd788 ( 312) CTGCCCTCCACATCATGGCTT 1 263252 ( 444) CTGCCCTCCACATCATGGCTT 1 9279 ( 277) CTTCCATCACCTCCATTGCTG 1 10010 ( 187) CGCCCCCCTCCACAATGGCTT 1 // -------------------------------------------------------------------------------- -------------------------------------------------------------------------------- Motif 1 position-specific scoring matrix -------------------------------------------------------------------------------- log-odds matrix: alength= 4 w= 21 n= 14057 bayes= 10.7782 E= 9.7e-016 -965 218 -965 -965 -965 -965 -90 176 79 -81 10 -105 -965 218 -965 -965 -965 218 -965 -965 -121 199 -965 -965 -965 -81 -965 176 -965 199 -965 -105 -121 151 -965 -5 -21 177 -965 -965 -965 218 -965 -965 38 -965 -965 127 -965 177 -965 -5 -121 118 -965 54 179 -965 -965 -965 -965 77 -965 127 -965 -965 110 95 -965 -965 210 -965 -965 218 -965 -965 -965 -965 -965 195 -965 -965 142 54 -------------------------------------------------------------------------------- -------------------------------------------------------------------------------- Motif 1 position-specific probability matrix -------------------------------------------------------------------------------- letter-probability matrix: alength= 4 w= 21 nsites= 8 E= 9.7e-016 0.000000 1.000000 0.000000 0.000000 0.000000 0.000000 0.125000 0.875000 0.500000 0.125000 0.250000 0.125000 0.000000 1.000000 0.000000 0.000000 0.000000 1.000000 0.000000 0.000000 0.125000 0.875000 0.000000 0.000000 0.000000 0.125000 0.000000 0.875000 0.000000 0.875000 0.000000 0.125000 0.125000 0.625000 0.000000 0.250000 0.250000 0.750000 0.000000 0.000000 0.000000 1.000000 0.000000 0.000000 0.375000 0.000000 0.000000 0.625000 0.000000 0.750000 0.000000 0.250000 0.125000 0.500000 0.000000 0.375000 1.000000 0.000000 0.000000 0.000000 0.000000 0.375000 0.000000 0.625000 0.000000 0.000000 0.500000 0.500000 0.000000 0.000000 1.000000 0.000000 0.000000 1.000000 0.000000 0.000000 0.000000 0.000000 0.000000 1.000000 0.000000 0.000000 0.625000 0.375000 -------------------------------------------------------------------------------- -------------------------------------------------------------------------------- Motif 1 regular expression -------------------------------------------------------------------------------- CT[AG]CCCTC[CT][CA]C[TA][CT][CT]A[TC][GT]GCT[GT] -------------------------------------------------------------------------------- Time 6.86 secs. ******************************************************************************** ******************************************************************************** MOTIF 2 MEME width = 21 sites = 14 llr = 212 E-value = 2.3e-008 ******************************************************************************** -------------------------------------------------------------------------------- Motif 2 Description -------------------------------------------------------------------------------- Simplified A :21:1138a5:4a8:146a7: pos.-specific C 7::1466::54::19151:39 probability G 342:11:2::46:::2:4::: matrix T :479511:::1::1161:::1 bits 2.2 2.0 1.7 * * * * 1.5 * * * * * Relative 1.3 * * * * * * * Entropy 1.1 * * *** ** * *** (21.9 bits) 0.9 * ** *** **** *** 0.7 * ** ********** ***** 0.4 ********************* 0.2 ********************* 0.0 --------------------- Multilevel CGTTTCCAAACGAACTCAAAC consensus GTG C AG CGA GAG C sequence A -------------------------------------------------------------------------------- -------------------------------------------------------------------------------- Motif 2 sites sorted by position p-value -------------------------------------------------------------------------------- Sequence name Start P-value Site ------------- ----- --------- --------------------- 9656 342 1.01e-09 CCAACGGCGG CGGTTCCGACCGAACTCAACC CTACCCTCCC 6614 342 1.01e-09 CCAACGGCGG CGGTTCCGACCGAACTCAACC CTACCCTCCC 11540 342 1.01e-09 CCAACGGCGG CGGTTCCGACCGAACTCAACC CTACCCTCCC 5669 463 1.34e-09 GCAACTAATT CTTTTCAAACGGAACTCCAAC CAAGCAGCAT 263201 288 6.24e-09 CACTATGAAA CGTTCAAAAAGGAACTAGAAC ACCTAGTACG 261696 437 6.24e-09 CACTATCAAA CGTTCAAAAAGGAACTAGAAC ACCTGGTACG 6604 479 4.90e-08 TCTATTGCTC GTTTTGCAAATAAACGCAAAC G 9259 325 1.44e-07 TCCAGAACGG CGTTCCAAACGAATCCAGAAC CAAACTGATG 10509 435 1.56e-07 TGCGTTTCAG CTTTTTCAAACGAACGCAACT CATTGTCTAC bd788 126 2.40e-07 GCAATAAACC CATTCCCAACGGACTATGAAC CGATGGAGGA 263252 258 2.40e-07 GCAATAAACC CATTCCCAACGGACTATGAAC CGATGGAGGA 263044 381 2.76e-07 AGATTGGTAT GTTCGCTAAACAAACTAAAAC AATCAATGAG 263605 381 3.37e-07 AGATTGGTAT GTTCACTAAACAAACTAAAAC GATCAATGAG bd813 479 6.23e-07 TGTTACACCT GAATTGCAAATAAACGCAAAC G -------------------------------------------------------------------------------- -------------------------------------------------------------------------------- Motif 2 block diagrams -------------------------------------------------------------------------------- SEQUENCE NAME POSITION P-VALUE MOTIF DIAGRAM ------------- ---------------- ------------- 9656 1e-09 341_[+2]_138 6614 1e-09 341_[+2]_138 11540 1e-09 341_[+2]_138 5669 1.3e-09 462_[+2]_17 263201 6.2e-09 287_[+2]_192 261696 6.2e-09 436_[+2]_43 6604 4.9e-08 478_[+2]_1 9259 1.4e-07 324_[+2]_155 10509 1.6e-07 434_[+2]_45 bd788 2.4e-07 125_[+2]_354 263252 2.4e-07 257_[+2]_222 263044 2.8e-07 380_[+2]_99 263605 3.4e-07 380_[+2]_99 bd813 6.2e-07 478_[+2]_1 -------------------------------------------------------------------------------- -------------------------------------------------------------------------------- Motif 2 in BLOCKS format -------------------------------------------------------------------------------- BL MOTIF 2 width=21 seqs=14 9656 ( 342) CGGTTCCGACCGAACTCAACC 1 6614 ( 342) CGGTTCCGACCGAACTCAACC 1 11540 ( 342) CGGTTCCGACCGAACTCAACC 1 5669 ( 463) CTTTTCAAACGGAACTCCAAC 1 263201 ( 288) CGTTCAAAAAGGAACTAGAAC 1 261696 ( 437) CGTTCAAAAAGGAACTAGAAC 1 6604 ( 479) GTTTTGCAAATAAACGCAAAC 1 9259 ( 325) CGTTCCAAACGAATCCAGAAC 1 10509 ( 435) CTTTTTCAAACGAACGCAACT 1 bd788 ( 126) CATTCCCAACGGACTATGAAC 1 263252 ( 258) CATTCCCAACGGACTATGAAC 1 263044 ( 381) GTTCGCTAAACAAACTAAAAC 1 263605 ( 381) GTTCACTAAACAAACTAAAAC 1 bd813 ( 479) GAATTGCAAATAAACGCAAAC 1 // -------------------------------------------------------------------------------- -------------------------------------------------------------------------------- Motif 2 position-specific scoring matrix -------------------------------------------------------------------------------- log-odds matrix: alength= 4 w= 21 n= 14057 bayes= 9.81393 E= 2.3e-008 -1045 170 30 -1045 -43 -1045 88 47 -201 -1045 -12 147 -1045 -62 -1045 173 -201 70 -170 95 -101 155 -70 -185 -2 138 -1045 -85 144 -1045 -12 -1045 179 -1045 -1045 -1045 79 118 -1045 -1045 -1045 96 88 -85 31 -1045 146 -1045 179 -1045 -1045 -1045 144 -62 -1045 -185 -1045 196 -1045 -85 -101 -162 -12 114 31 118 -1045 -85 98 -162 62 -1045 179 -1045 -1045 -1045 131 38 -1045 -1045 -1045 208 -1045 -185 -------------------------------------------------------------------------------- -------------------------------------------------------------------------------- Motif 2 position-specific probability matrix -------------------------------------------------------------------------------- letter-probability matrix: alength= 4 w= 21 nsites= 14 E= 2.3e-008 0.000000 0.714286 0.285714 0.000000 0.214286 0.000000 0.428571 0.357143 0.071429 0.000000 0.214286 0.714286 0.000000 0.142857 0.000000 0.857143 0.071429 0.357143 0.071429 0.500000 0.142857 0.642857 0.142857 0.071429 0.285714 0.571429 0.000000 0.142857 0.785714 0.000000 0.214286 0.000000 1.000000 0.000000 0.000000 0.000000 0.500000 0.500000 0.000000 0.000000 0.000000 0.428571 0.428571 0.142857 0.357143 0.000000 0.642857 0.000000 1.000000 0.000000 0.000000 0.000000 0.785714 0.142857 0.000000 0.071429 0.000000 0.857143 0.000000 0.142857 0.142857 0.071429 0.214286 0.571429 0.357143 0.500000 0.000000 0.142857 0.571429 0.071429 0.357143 0.000000 1.000000 0.000000 0.000000 0.000000 0.714286 0.285714 0.000000 0.000000 0.000000 0.928571 0.000000 0.071429 -------------------------------------------------------------------------------- -------------------------------------------------------------------------------- Motif 2 regular expression -------------------------------------------------------------------------------- [CG][GTA][TG]T[TC]C[CA][AG]A[AC][CG][GA]AAC[TG][CA][AG]A[AC]C -------------------------------------------------------------------------------- Time 13.81 secs. ******************************************************************************** ******************************************************************************** MOTIF 3 MEME width = 21 sites = 10 llr = 178 E-value = 2.4e-009 ******************************************************************************** -------------------------------------------------------------------------------- Motif 3 Description -------------------------------------------------------------------------------- Simplified A a::::1a23:3:4:9:31:96 pos.-specific C :9::31:8:17:2::53::1: probability G ::a726::79:4:a14:4a:1 matrix T :1:352:::::64::145::3 bits 2.2 * * * 2.0 * * * 1.7 *** * * * * 1.5 *** * * * * Relative 1.3 *** ** * ** ** Entropy 1.1 **** ****** ** ** (25.7 bits) 0.9 **** ****** *** ** 0.7 ***** ****** *** **** 0.4 ********************* 0.2 ********************* 0.0 --------------------- Multilevel ACGGTGACGGCTAGACTTGAA consensus TCT AA AGT GAG T sequence G C C -------------------------------------------------------------------------------- -------------------------------------------------------------------------------- Motif 3 sites sorted by position p-value -------------------------------------------------------------------------------- Sequence name Start P-value Site ------------- ----- --------- --------------------- 9656 397 1.32e-11 CTAGACAGCG ACGGTGACGGCGAGAGTGGAA CCGGAGCATC 6614 397 1.32e-11 CTAGACAGCG ACGGTGACGGCGAGAGTGGAA CCGGAGCATC 11540 397 1.32e-11 CTAGACAGCG ACGGTGACGGCGAGAGTGGAA CCGGAGCATC 263605 457 2.97e-09 GACTCGATCA ACGGCTAAGGCTCGACATGAA AATATAGTTG 263044 457 2.97e-09 GACTCGATCA ACGGCTAAGGCTCGACATGAA AATGTAGTTG 10536 460 4.22e-09 GGTTTCAAGC ACGTGGACAGATTGACTTGAT TGACATGCAG bd1098 213 4.98e-09 GGTTTCATGC ACGTGGACAGATTGACCTGAT TGACATCCAG 2000 113 2.90e-08 GTGGTTTGAT ACGGCAACGCCGAGACCAGAA GTGTACAAGG 10509 270 5.29e-08 GGGGATGTTG ATGTTGACAGATTGAGAGGAG TGGAGATTCA bd1097 17 6.31e-08 AGTCTGACAC ACGGTCACGGCTTGGTCTGCT GTTAAGCTCA -------------------------------------------------------------------------------- -------------------------------------------------------------------------------- Motif 3 block diagrams -------------------------------------------------------------------------------- SEQUENCE NAME POSITION P-VALUE MOTIF DIAGRAM ------------- ---------------- ------------- 9656 1.3e-11 396_[+3]_83 6614 1.3e-11 396_[+3]_83 11540 1.3e-11 396_[+3]_83 263605 3e-09 456_[+3]_23 263044 3e-09 456_[+3]_23 10536 4.2e-09 459_[+3]_20 bd1098 5e-09 212_[+3]_267 2000 2.9e-08 112_[+3]_367 10509 5.3e-08 269_[+3]_210 bd1097 6.3e-08 16_[+3]_463 -------------------------------------------------------------------------------- -------------------------------------------------------------------------------- Motif 3 in BLOCKS format -------------------------------------------------------------------------------- BL MOTIF 3 width=21 seqs=10 9656 ( 397) ACGGTGACGGCGAGAGTGGAA 1 6614 ( 397) ACGGTGACGGCGAGAGTGGAA 1 11540 ( 397) ACGGTGACGGCGAGAGTGGAA 1 263605 ( 457) ACGGCTAAGGCTCGACATGAA 1 263044 ( 457) ACGGCTAAGGCTCGACATGAA 1 10536 ( 460) ACGTGGACAGATTGACTTGAT 1 bd1098 ( 213) ACGTGGACAGATTGACCTGAT 1 2000 ( 113) ACGGCAACGCCGAGACCAGAA 1 10509 ( 270) ATGTTGACAGATTGAGAGGAG 1 bd1097 ( 17) ACGGTCACGGCTTGGTCTGCT 1 // -------------------------------------------------------------------------------- -------------------------------------------------------------------------------- Motif 3 position-specific scoring matrix -------------------------------------------------------------------------------- log-odds matrix: alength= 4 w= 21 n= 14057 bayes= 10.7077 E= 2.4e-009 179 -997 -997 -997 -997 203 -997 -137 -997 -997 210 -997 -997 -997 159 21 -997 45 -22 95 -153 -114 137 -37 179 -997 -997 -997 -53 186 -997 -997 6 -997 159 -997 -997 -114 195 -997 6 167 -997 -997 -997 -997 78 121 47 -14 -997 63 -997 -997 210 -997 164 -997 -122 -997 -997 118 78 -137 6 45 -997 63 -153 -997 78 95 -997 -997 210 -997 164 -114 -997 -997 105 -997 -122 21 -------------------------------------------------------------------------------- -------------------------------------------------------------------------------- Motif 3 position-specific probability matrix -------------------------------------------------------------------------------- letter-probability matrix: alength= 4 w= 21 nsites= 10 E= 2.4e-009 1.000000 0.000000 0.000000 0.000000 0.000000 0.900000 0.000000 0.100000 0.000000 0.000000 1.000000 0.000000 0.000000 0.000000 0.700000 0.300000 0.000000 0.300000 0.200000 0.500000 0.100000 0.100000 0.600000 0.200000 1.000000 0.000000 0.000000 0.000000 0.200000 0.800000 0.000000 0.000000 0.300000 0.000000 0.700000 0.000000 0.000000 0.100000 0.900000 0.000000 0.300000 0.700000 0.000000 0.000000 0.000000 0.000000 0.400000 0.600000 0.400000 0.200000 0.000000 0.400000 0.000000 0.000000 1.000000 0.000000 0.900000 0.000000 0.100000 0.000000 0.000000 0.500000 0.400000 0.100000 0.300000 0.300000 0.000000 0.400000 0.100000 0.000000 0.400000 0.500000 0.000000 0.000000 1.000000 0.000000 0.900000 0.100000 0.000000 0.000000 0.600000 0.000000 0.100000 0.300000 -------------------------------------------------------------------------------- -------------------------------------------------------------------------------- Motif 3 regular expression -------------------------------------------------------------------------------- ACG[GT][TCG][GT]A[CA][GA]G[CA][TG][ATC]GA[CG][TAC][TG]GA[AT] -------------------------------------------------------------------------------- Time 20.53 secs. ******************************************************************************** ******************************************************************************** SUMMARY OF MOTIFS ******************************************************************************** -------------------------------------------------------------------------------- Combined block diagrams: non-overlapping sites with p-value < 0.0001 -------------------------------------------------------------------------------- SEQUENCE NAME COMBINED P-VALUE MOTIF DIAGRAM ------------- ---------------- ------------- 10010 1.68e-05 186_[+1(2.15e-09)]_293 10509 2.93e-07 269_[+3(5.29e-08)]_144_\ [+2(1.56e-07)]_45 10536 3.80e-05 459_[+3(4.22e-09)]_20 10713 2.96e-01 500 11540 2.78e-21 341_[+2(1.01e-09)]_[+1(1.22e-12)]_\ 13_[+3(1.32e-11)]_83 2000 1.76e-04 112_[+3(2.90e-08)]_367 261696 5.06e-05 436_[+2(6.24e-09)]_43 262191 2.23e-01 500 263044 3.13e-08 380_[+2(2.76e-07)]_55_\ [+3(2.97e-09)]_23 263201 4.76e-05 287_[+2(6.24e-09)]_192 263252 3.17e-09 257_[+2(2.40e-07)]_165_\ [+1(1.82e-10)]_36 263605 2.74e-08 380_[+2(3.37e-07)]_55_\ [+3(2.97e-09)]_23 264036 2.65e-01 500 5669 1.11e-05 462_[+2(1.34e-09)]_17 6604 1.19e-03 478_[+2(4.90e-08)]_1 6614 2.78e-21 341_[+2(1.01e-09)]_[+1(1.22e-12)]_\ 13_[+3(1.32e-11)]_83 7301 2.58e-01 500 7394 9.95e-01 500 7833 9.08e-02 500 9241 9.22e-01 500 9259 2.48e-04 324_[+2(1.44e-07)]_155 9279 1.95e-06 276_[+1(4.05e-10)]_203 9306 8.60e-01 500 9309 8.09e-02 379_[+1(5.28e-05)]_100 9656 2.78e-21 341_[+2(1.01e-09)]_[+1(1.22e-12)]_\ 13_[+3(1.32e-11)]_83 bd1097 7.09e-04 16_[+3(6.31e-08)]_463 bd1098 8.37e-05 212_[+3(4.98e-09)]_267 bd788 3.17e-09 125_[+2(2.40e-07)]_165_\ [+1(1.82e-10)]_168 bd810 7.03e-01 157 bd813 2.97e-10 325_[+1(4.67e-11)]_132_\ [+2(6.23e-07)]_1 -------------------------------------------------------------------------------- ******************************************************************************** ******************************************************************************** Stopped because nmotifs = 3 reached. ******************************************************************************** CPU: seaotter.hsd1.wa.comcast.net ********************************************************************************