Rv0006 DNA gyrase subunit A (EC 5.99.1.3)

Summary

Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv0006 gyrA DNA gyrase subunit A (EC 5.99.1.3) CDS 7302 9818 + 2 517 838 FALSE

Rv0006 (DNA gyrase subunit A (EC 5.99.1.3)) is predicted to be co-regulated in modules bicluster_0142 with residual 0.59 and bicluster_0162 with residual 0.54.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 420.00 and 7,300.00 for bicluster_0142 and 45.00 and 5,400.00 for bicluster_0162 respectively.

These modules are enriched for following go terms: DNA topological change, glutamate metabolic process, dicarboxylic acid biosynthetic process, DNA conformation change, DNA topoisomerase activity cysteine biosynthetic process from serin..., cysteine biosynthetic process, cysteine metabolic process, L-serine metabolic process.

This gene is found to be for growth on cholesterol.

Mutant available?:

Product (LegacyBRC) Product (RefSeq)
DNA gyrase subunit A DNA gyrase subunit A
Operon # Operon
3 -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15607148 NP_214520.1 Run
GO:0003918

DNA topoisomerase (ATP-hydrolyzing) activity

DNA topoisomerase (ATP-hydrolyzing) activity

Details: 
Catalysis of a DNA topological transformation by transiently cleaving a pair of complementary DNA strands to form a gate through which a second double-stranded DNA segment is passed, after which the severed strands in the first DNA segment are rejoined; product release is coupled to ATP binding and hydrolysis; changes the linking number in multiples of 2.
GO Category: 
molecular_function
2
Total items in this category:  
GO:0000287

magnesium ion binding

magnesium ion binding

Details: 
Interacting selectively and non-covalently with magnesium (Mg) ions.
GO Category: 
molecular_function
52
Total items in this category:  
GO:0003918

DNA topoisomerase (ATP-hydrolyzing) activity

DNA topoisomerase (ATP-hydrolyzing) activity

Details: 
Catalysis of a DNA topological transformation by transiently cleaving a pair of complementary DNA strands to form a gate through which a second double-stranded DNA segment is passed, after which the severed strands in the first DNA segment are rejoined; product release is coupled to ATP binding and hydrolysis; changes the linking number in multiples of 2.
GO Category: 
molecular_function
2
Total items in this category:  
GO:0005515

protein binding

protein binding

Details: 
Interacting selectively and non-covalently with any protein or protein complex (a complex of two or more proteins that may include other nonprotein molecules).
GO Category: 
molecular_function
135
Total items in this category:  
GO:0005524

ATP binding

ATP binding

Details: 
Interacting selectively and non-covalently with ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
GO Category: 
molecular_function
58
Total items in this category:  
GO:0005886

plasma membrane

plasma membrane

Details: 
The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
cellular_component
1284
Total items in this category:  
GO:0046982

protein heterodimerization activity

protein heterodimerization activity

Details: 
Interacting selectively and non-covalently with a nonidentical protein to form a heterodimer.
GO Category: 
molecular_function
2
Total items in this category:  
No TFOE experiment results were found

Quantitative Proteomics Data

t-test p-value Cholesterol/Glycerol Ratio
NA NA

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: