Rv0404 Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3)

Summary

Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv0404 fadD30 Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3) CDS 483977 485734 + 1 758 585 FALSE

Rv0404 (Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3)) is predicted to be co-regulated in modules bicluster_0456 with residual 0.56 and bicluster_0596 with residual 0.61.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 190.00 and 9,400.00 for bicluster_0456 and 0.00 and 0.03 for bicluster_0596 respectively.

These modules are enriched for following go terms: .

This gene is found to be for growth on cholesterol.

Mutant available?: Yes

Last update: 10/16/2017 - 02:57
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-18011 MT0417 1122
Product (LegacyBRC) Product (RefSeq)
PROBABLE FATTY-ACID-CoA LIGASE FADD30 [FATTY-ACID-CoA SYNTHETASE] [FATTY-ACID-CoA SYNTHASE] acyl-CoA synthetase
Operon # Operon
275 -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Long-chain-fatty-acid--CoA ligase Fatty acid metabolism
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15607545 NP_214918.1 Run
GO:0004467

long-chain fatty acid-CoA ligase activity

long-chain fatty acid-CoA ligase activity

Details: 
Catalysis of the reaction: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate + an acyl-CoA; a long-chain fatty acid is a fatty acid with a chain length between C13 and C22.
GO Category: 
molecular_function
13
Total items in this category:  
GO:0040007

growth

growth

Details: 
The increase in size or mass of an entire organism, a part of an organism or a cell.
GO Category: 
biological_process
621
Total items in this category:  
GO:0070566

adenylyltransferase activity

adenylyltransferase activity

Details: 
Catalysis of the transfer of an adenylyl group to an acceptor.
GO Category: 
molecular_function
6
Total items in this category:  
No TFOE experiment results were found

Quantitative Proteomics Data

t-test p-value Cholesterol/Glycerol Ratio
0.710000 1.80

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: