Rv0462 Dihydrolipoamide dehydrogenase (EC 1.8.1.4)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv0462 lpd Dihydrolipoamide dehydrogenase (EC 1.8.1.4) CDS 552614 554008 + 1 395 464 FALSE

Rv0462 (Dihydrolipoamide dehydrogenase (EC 1.8.1.4)) is predicted to be co-regulated in modules bicluster_0079 with residual 0.57 and bicluster_0458 with residual 0.58.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.70 and 11.00 for bicluster_0079 and 0.15 and 30,000.00 for bicluster_0458 respectively.

These modules are enriched for following go terms: potassium ion transport organonitrogen compound metabolic proces..., primary metabolic process, small molecule metabolic process, small molecule biosynthetic process, single-organism biosynthetic process, organonitrogen compound biosynthetic pro....

This gene is found to be for growth on cholesterol.

Mutant available?:

Product (LegacyBRC) Product (RefSeq)
Dihydrolipoyl dehydrogenase dihydrolipoamide dehydrogenase
Operon # Operon
310 -
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Glycolysis / Gluconeogenesis

35
Total items in this category:  

KEGG

Citrate cycle (TCA cycle)

35
Total items in this category:  

KEGG

Glycine, serine and threonine metabolism

27
Total items in this category:  

KEGG

Valine, leucine and isoleucine degradation

60
Total items in this category:  

KEGG

Pyruvate metabolism

40
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  

KEGG

Biosynthesis of secondary metabolites

326
Total items in this category:  

KEGG

Microbial metabolism in diverse environments

296
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15607603 NP_214976.1 Run
GO:0004148

dihydrolipoyl dehydrogenase activity

dihydrolipoyl dehydrogenase activity

Details: 
Catalysis of the reaction: protein N6-(dihydrolipoyl)lysine + NAD+ = protein N6-(lipoyl)lysine + NADH + H+.
GO Category: 
molecular_function
2
Total items in this category:  
GO:0004148

dihydrolipoyl dehydrogenase activity

dihydrolipoyl dehydrogenase activity

Details: 
Catalysis of the reaction: protein N6-(dihydrolipoyl)lysine + NAD+ = protein N6-(lipoyl)lysine + NADH + H+.
GO Category: 
molecular_function
2
Total items in this category:  
GO:0005515

protein binding

protein binding

Details: 
Interacting selectively and non-covalently with any protein or protein complex (a complex of two or more proteins that may include other nonprotein molecules).
GO Category: 
molecular_function
135
Total items in this category:  
GO:0005576

extracellular region

extracellular region

Details: 
The space external to the outermost structure of a cell. For cells without external protective or external encapsulating structures this refers to space outside of the plasma membrane. This term covers the host cell environment outside an intracellular parasite.
GO Category: 
cellular_component
272
Total items in this category:  
GO:0005829

cytosol

cytosol

Details: 
The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
GO Category: 
cellular_component
371
Total items in this category:  
GO:0005886

plasma membrane

plasma membrane

Details: 
The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
cellular_component
1284
Total items in this category:  
GO:0016655

oxidoreductase activity, acting on NADH or NADPH, quinone or similar compound as acceptor

oxidoreductase activity, acting on NADH or NADPH, quinone or similar compound as acceptor

Details: 
Catalysis of an oxidation-reduction (redox) reaction in which NADH or NADPH acts as a hydrogen or electron donor and reduces a quinone or a similar acceptor molecule.
GO Category: 
molecular_function
3
Total items in this category:  
GO:0040007

growth

growth

Details: 
The increase in size or mass of an entire organism, a part of an organism or a cell.
GO Category: 
biological_process
621
Total items in this category:  
GO:0042803

protein homodimerization activity

protein homodimerization activity

Details: 
Interacting selectively and non-covalently with an identical protein to form a homodimer.
GO Category: 
molecular_function
83
Total items in this category:  
GO:0045254

pyruvate dehydrogenase complex

pyruvate dehydrogenase complex

Details: 
Complex that carries out the oxidative decarboxylation of pyruvate to form acetyl-CoA; comprises subunits possessing three catalytic activities: pyruvate dehydrogenase (E1), dihydrolipoamide S-acetyltransferase (E2), and dihydrolipoamide dehydrogenase (E3).
GO Category: 
cellular_component
4
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
NA NA

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: