Rv0803 Phosphoribosylformylglycinamidine synthase, synthetase subunit (EC 6.3.5.3)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv0803 purL Phosphoribosylformylglycinamidine synthase, synthetase subunit (EC 6.3.5.3) CDS 895820 898084 + 2 265 754 FALSE

Rv0803 (Phosphoribosylformylglycinamidine synthase, synthetase subunit (EC 6.3.5.3)) is predicted to be co-regulated in modules bicluster_0187 with residual 0.50 and bicluster_0407 with residual 0.55.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.01 and 0.16 for bicluster_0187 and 0.10 and 1.70 for bicluster_0407 respectively.

These modules are enriched for following go terms: organophosphate biosynthetic process, phospholipid metabolic process, phospholipid biosynthetic process GTPase activity, GTP binding, guanyl nucleotide binding, guanyl ribonucleotide binding.

This gene is found to be for growth on cholesterol.

Mutant available?:

Product (LegacyBRC) Product (RefSeq)
Phosphoribosylformylglycinamidine synthase II phosphoribosylformylglycinamidine synthase II
Operon # Operon
530 -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Phosphoribosylformylglycinamidine synthase Purine metabolism
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Purine metabolism

65
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  

KEGG

Biosynthesis of secondary metabolites

326
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15607943 NP_215318.1 Run
GO:0004642

phosphoribosylformylglycinamidine synthase activity

phosphoribosylformylglycinamidine synthase activity

Details: 
Catalysis of the reaction: N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide + L-glutamine + ATP + H(2)O = 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate + ADP + 2 H(+) + phosphate.
GO Category: 
molecular_function
3
Total items in this category:  
GO:0006164

purine nucleotide biosynthetic process

purine nucleotide biosynthetic process

Details: 
The chemical reactions and pathways resulting in the formation of a purine nucleotide, a compound consisting of nucleoside (a purine base linked to a deoxyribose or ribose sugar) esterified with a phosphate group at either the 3' or 5'-hydroxyl group of the sugar.
GO Category: 
biological_process
3
Total items in this category:  
GO:0040007

growth

growth

Details: 
The increase in size or mass of an entire organism, a part of an organism or a cell.
GO Category: 
biological_process
621
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
NA NA

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: