Rv0823c tRNA dihydrouridine synthase B (EC 1.-.-.-)

Summary

Product Feature Type Start End Strand Length AA Length is TF
Rv0823c tRNA dihydrouridine synthase B (EC 1.-.-.-) CDS 916477 917646 - 1 170 389 FALSE

Rv0823c (tRNA dihydrouridine synthase B (EC 1.-.-.-)) is predicted to be co-regulated in modules bicluster_0426 with residual 0.50 and bicluster_0478 with residual 0.49.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 950.00 and 11,000.00 for bicluster_0426 and 0.00 and 0.09 for bicluster_0478 respectively.

These modules are enriched for following go terms: nicotinamide nucleotide metabolic proces..., oxidoreduction coenzyme metabolic proces..., pyridine nucleotide metabolic process, pyridine-containing compound metabolic p..., RNA binding single-organism process, transport, establishment of localization, localization, arsenite transmembrane transporter activ..., anion transmembrane transporter activity.

This gene is found to be for growth on cholesterol.

Mutant available?: Yes

BASS Score New Primary UTR Primary TSS Re-Annotated Start Tuberculist Annotated Start
-0.255 325 917935 917610 917646
Last update: 11/20/2020 - 12:11
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-13485 MT0845 42
Product (LegacyBRC) Product (RefSeq)
Probable tRNA-dihydrouridine synthase transcriptional regulatory protein
Operon # Operon
545
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15607963 NP_215338.1 Run
No TFOE experiment results were found

Quantitative Proteomics Data

t-test p-value Cholesterol/Glycerol Ratio
0.640000 1.20

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated: 0.680757
p-value INH: 0.832328
Displaying 1 - 17 of 17
Condition Count Day Doublings Fitness U.I Plots
D0U 27 0 0.00 14.81 U
D3I 3 3 3.83 15.00 I
D3U 3 3 3.83 14.75 U
D5I 9 5 6.00 14.97 I
D5U 17 5 6.00 15.00 U
D7I 18 7 8.14 15.04 I
D7U 19 7 8.14 14.63 U
D14I 4 14 15.63 13.16 I
D14U 4 14 15.63 13.53 U
D17I 3 17 19.15 12.00 I
D17U 3 17 19.15 12.91 U
D21I 4 21 23.23 12.04 I
D21U 4 21 23.23 13.24 U
D24I 3 24 26.60 10.82 I
D24U 3 24 26.60 12.66 U
D28I 4 28 30.61 10.98 I
D28U 4 28 30.61 13.01 U