Rv0848 Cysteine synthase (EC 2.5.1.47)

Summary

Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv0848 cysK2 Cysteine synthase (EC 2.5.1.47) CDS 944938 946056 + 1 119 372 FALSE

Rv0848 (Cysteine synthase (EC 2.5.1.47)) is predicted to be co-regulated in modules bicluster_0073 with residual 0.60 and bicluster_0337 with residual 0.50.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.00 and 0.00 for bicluster_0073 and 0.00 and 0.00 for bicluster_0337 respectively.

These modules are enriched for following go terms: aspartate carbamoyltransferase activity.

This gene is found to be for growth on cholesterol.

Mutant available?:

BASS Score Re-Annotated Start Tuberculist Annotated Start
-0.442 944842 944938
Product (LegacyBRC) Product (RefSeq)
POSSIBLE CYSTEINE SYNTHASE A CYSK2 [O-ACETYLSERINE SULFHYDRYLASE] [O-ACETYLSERINE [THIOL]-LYASE] [CSASE] cysteine synthase A CysK2
Operon # Operon
568 - -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Cysteine synthase Cysteine and methionine metabolism, Sulfur metabolism
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Cysteine and methionine metabolism

28
Total items in this category:  

KEGG

Selenocompound metabolism

40
Total items in this category:  

KEGG

Sulfur metabolism

12
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  

KEGG

Microbial metabolism in diverse environments

296
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
57116791 YP_177762.1 Run
GO:0004124

cysteine synthase activity

cysteine synthase activity

Details: 
Catalysis of the reaction: O3-acetyl-L-serine + hydrogen sulfide = L-cysteine + acetate.
GO Category: 
molecular_function
4
Total items in this category:  
GO:0005618

cell wall

cell wall

Details: 
The rigid or semi-rigid envelope lying outside the cell membrane of plant, fungal, and most prokaryotic cells, maintaining their shape and protecting them from osmotic lysis. In plants it is made of cellulose and, often, lignin; in fungi it is composed largely of polysaccharides; in bacteria it is composed of peptidoglycan.
GO Category: 
cellular_component
320
Total items in this category:  
No TFOE experiment results were found

Quantitative Proteomics Data

t-test p-value Cholesterol/Glycerol Ratio
0.890000 1.44

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: