Rv0975c Isovaleryl-CoA dehydrogenase (EC 1.3.99.10)

Summary

Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv0975c fadE13 Isovaleryl-CoA dehydrogenase (EC 1.3.99.10) CDS 1087348 1088496 - 1 149 382 FALSE

Rv0975c (Isovaleryl-CoA dehydrogenase (EC 1.3.99.10)) is predicted to be co-regulated in modules bicluster_0326 with residual 0.49 and bicluster_0394 with residual 0.56.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.00 and 0.40 for bicluster_0326 and 26.00 and 340.00 for bicluster_0394 respectively.

These modules are enriched for following go terms: cellular protein metabolic process, macromolecular complex, cytoplasmic part.

This gene is found to be for growth on cholesterol.

Mutant available?: Yes

Last update: 10/16/2017 - 11:08
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-18136 MT1003 1476
Product (LegacyBRC) Product (RefSeq)
PROBABLE ACYL-CoA DEHYDROGENASE FADE13 acyl-CoA dehydrogenase FADE13
Operon # Operon
649 - - - - -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Isovaleryl-CoA dehydrogenase Valine, leucine and isoleucine degradation
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Fatty acid metabolism

51
Total items in this category:  

KEGG

Valine, leucine and isoleucine degradation

60
Total items in this category:  

KEGG

beta-Alanine metabolism

37
Total items in this category:  

KEGG

Propanoate metabolism

62
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  

KEGG

Biosynthesis of secondary metabolites

326
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15608115 NP_215490.1 Run
GO:0008470

isovaleryl-CoA dehydrogenase activity

isovaleryl-CoA dehydrogenase activity

Details: 
Catalysis of the reaction: 3-methylbutanoyl-CoA + ETF = 3-methylbut-2-enoyl-CoA + reduced ETF.
GO Category: 
molecular_function
3
Total items in this category:  
No TFOE experiment results were found

Quantitative Proteomics Data

t-test p-value Cholesterol/Glycerol Ratio
0.100000 0.83

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: