Rv1099c Fructose-1,6-bisphosphatase, GlpX type (EC 3.1.3.11)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv1099c glpX Fructose-1,6-bisphosphatase, GlpX type (EC 3.1.3.11) CDS 1227596 1228582 - 987 328 FALSE

Rv1099c (Fructose-1,6-bisphosphatase, GlpX type (EC 3.1.3.11)) is predicted to be co-regulated in modules bicluster_0066 with residual 0.56 and bicluster_0255 with residual 0.52.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 47.00 and 3,300.00 for bicluster_0066 and 0.00 and 3.80 for bicluster_0255 respectively.

These modules are enriched for following go terms: glycerol ether metabolic process, ether metabolic process, protein disulfide oxidoreductase activit..., disulfide oxidoreductase activity .

This gene is found to be for growth on cholesterol.

Mutant available?:

BASS Score New Primary UTR Primary TSS Re-Annotated Start Tuberculist Annotated Start
-0.576 -102 1228684 1228786 1228684
Product (LegacyBRC) Product (RefSeq)
Putative uncharacterized protein fructose 1,6-bisphosphatase II
Operon # Operon
742 - -
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Glycolysis / Gluconeogenesis

35
Total items in this category:  

KEGG

Pentose phosphate pathway

19
Total items in this category:  

KEGG

Fructose and mannose metabolism

18
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  

KEGG

Biosynthesis of secondary metabolites

326
Total items in this category:  

KEGG

Microbial metabolism in diverse environments

296
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15608239 NP_215615.1 Run
GO:0042132

fructose 1,6-bisphosphate 1-phosphatase activity

fructose 1,6-bisphosphate 1-phosphatase activity

Details: 
Catalysis of the reaction: D-fructose 1,6-bisphosphate + H2O = D-fructose 6-phosphate + phosphate.
GO Category: 
molecular_function
2
Total items in this category:  
GO:0005829

cytosol

cytosol

Details: 
The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
GO Category: 
cellular_component
371
Total items in this category:  
GO:0006094

gluconeogenesis

gluconeogenesis

Details: 
The formation of glucose from noncarbohydrate precursors, such as pyruvate, amino acids and glycerol.
GO Category: 
biological_process
1
Total items in this category:  
GO:0042132

fructose 1,6-bisphosphate 1-phosphatase activity

fructose 1,6-bisphosphate 1-phosphatase activity

Details: 
Catalysis of the reaction: D-fructose 1,6-bisphosphate + H2O = D-fructose 6-phosphate + phosphate.
GO Category: 
molecular_function
2
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.410000 8.61

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: