Rv1122 6-phosphogluconate dehydrogenase, decarboxylating (EC 1.1.1.44)

Summary

Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv1122 gnd2 6-phosphogluconate dehydrogenase, decarboxylating (EC 1.1.1.44) CDS 1245129 1246151 + 1 023 340 FALSE

Rv1122 (6-phosphogluconate dehydrogenase, decarboxylating (EC 1.1.1.44)) is predicted to be co-regulated in modules bicluster_0191 with residual 0.46 and bicluster_0444 with residual 0.53.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.27 and 2.30 for bicluster_0191 and 230.00 and 23,000.00 for bicluster_0444 respectively.

These modules are enriched for following go terms: cellular component organization, cellular component biogenesis, cellular component organization or bioge....

This gene is found to be for growth on cholesterol.

Mutant available?:

Product (LegacyBRC) Product (RefSeq)
PROBABLE 6-PHOSPHOGLUCONATE DEHYDROGENASE DECARBOXYLATING GND2 6-phosphogluconate dehydrogenase-like protein
Operon # Operon
758 -
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Pentose phosphate pathway

19
Total items in this category:  

KEGG

Glutathione metabolism

14
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  

KEGG

Biosynthesis of secondary metabolites

326
Total items in this category:  

KEGG

Microbial metabolism in diverse environments

296
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15608262 NP_215638.1 Run
GO:0004616

phosphogluconate dehydrogenase (decarboxylating) activity

phosphogluconate dehydrogenase (decarboxylating) activity

Details: 
Catalysis of the reaction: 6-phospho-D-gluconate + NADP+ = D-ribulose 5-phosphate + CO2 + NADPH + H+.
GO Category: 
molecular_function
2
Total items in this category:  
GO:0005886

plasma membrane

plasma membrane

Details: 
The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
cellular_component
1284
Total items in this category:  
GO:0040007

growth

growth

Details: 
The increase in size or mass of an entire organism, a part of an organism or a cell.
GO Category: 
biological_process
621
Total items in this category:  
No TFOE experiment results were found

Quantitative Proteomics Data

t-test p-value Cholesterol/Glycerol Ratio
NA NA

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: