Rv1297 Transcription termination factor Rho

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv1297 rho Transcription termination factor Rho CDS 1453204 1455012 + 1 809 602 FALSE

Rv1297 (Transcription termination factor Rho) is predicted to be co-regulated in modules bicluster_0162 with residual 0.54 and bicluster_0257 with residual 0.53.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 45.00 and 5,400.00 for bicluster_0162 and 710.00 and 1,500.00 for bicluster_0257 respectively.

These modules are enriched for following go terms: cysteine biosynthetic process from serin..., cysteine biosynthetic process, cysteine metabolic process, L-serine metabolic process phosphate-containing compound metabolic ..., phosphorus metabolic process.

This gene is found to be for growth on cholesterol.

Mutant available?:

Product (LegacyBRC) Product (RefSeq)
Transcription termination factor rho transcription termination factor Rho
Operon # Operon
882
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

RNA degradation

10
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15608437 NP_215813.1 Run
GO:0016469

proton-transporting two-sector ATPase complex

proton-transporting two-sector ATPase complex

Details: 
A large protein complex that catalyzes the synthesis or hydrolysis of ATP by a rotational mechanism, coupled to the transport of protons across a membrane. The complex comprises a membrane sector (F0, V0, or A0) that carries out proton transport and a cytoplasmic compartment sector (F1, V1, or A1) that catalyzes ATP synthesis or hydrolysis. Two major types have been characterized: V-type ATPases couple ATP hydrolysis to the transport of protons across a concentration gradient, whereas F-type ATPases, also known as ATP synthases, normally run in the reverse direction to utilize energy from a proton concentration or electrochemical gradient to synthesize ATP. A third type, A-type ATPases have been found in archaea, and are closely related to eukaryotic V-type ATPases but are reversible.
GO Category: 
cellular_component
1
Total items in this category:  
GO:0003715

transcription termination factor activity

transcription termination factor activity

Details: 
OBSOLETE. Any activity that brings about termination of transcription.
GO Category: 
molecular_function
1
Total items in this category:  
GO:0006353

DNA-dependent transcription, termination

DNA-dependent transcription, termination

Details: 
The cellular process that completes DNA-dependent transcription; the formation of phosphodiester bonds ceases, the RNA-DNA hybrid dissociates, and RNA polymerase releases the DNA.
GO Category: 
biological_process
1
Total items in this category:  
GO:0003723

RNA binding

RNA binding

Details: 
Interacting selectively and non-covalently with an RNA molecule or a portion thereof.
GO Category: 
molecular_function
16
Total items in this category:  
GO:0005524

ATP binding

ATP binding

Details: 
Interacting selectively and non-covalently with ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
GO Category: 
molecular_function
58
Total items in this category:  
GO:0015986

ATP synthesis coupled proton transport

ATP synthesis coupled proton transport

Details: 
The transport of protons across a membrane to generate an electrochemical gradient (proton-motive force) that powers ATP synthesis.
GO Category: 
biological_process
1
Total items in this category:  
GO:0005618

cell wall

cell wall

Details: 
The rigid or semi-rigid envelope lying outside the cell membrane of plant, fungal, and most prokaryotic cells, maintaining their shape and protecting them from osmotic lysis. In plants it is made of cellulose and, often, lignin; in fungi it is composed largely of polysaccharides; in bacteria it is composed of peptidoglycan.
GO Category: 
cellular_component
320
Total items in this category:  
GO:0005886

plasma membrane

plasma membrane

Details: 
The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
cellular_component
1284
Total items in this category:  
GO:0040007

growth

growth

Details: 
The increase in size or mass of an entire organism, a part of an organism or a cell.
GO Category: 
biological_process
621
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.000000 0.01

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: