Rv1377c Methyltransferase (EC 2.1.1.-)

Summary

Product Feature Type Start End Strand Length AA Length is TF
Rv1377c Methyltransferase (EC 2.1.1.-) CDS 1550579 1551217 - 639 212 FALSE

Rv1377c (Methyltransferase (EC 2.1.1.-)) is predicted to be co-regulated in modules bicluster_0137 with residual 0.49 and bicluster_0328 with residual 0.36.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 850.00 and 1,200.00 for bicluster_0137 and 94.00 and 3,700.00 for bicluster_0328 respectively.

These modules are enriched for following go terms: 'de novo' pyrimidine nucleobase biosynth..., pyrimidine-containing compound biosynthe..., pyrimidine-containing compound metabolic..., organonitrogen compound metabolic proces..., nucleobase-containing small molecule met..., carbon-nitrogen ligase activity, with gl... .

This gene is found to be for growth on cholesterol.

Mutant available?: Yes

Last update: 10/16/2017 - 11:16
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-14926 MT1421 275
Product (LegacyBRC) Product (RefSeq)
PUTATIVE TRANSFERASE putative transferase
Operon # Operon
927 -
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15608517 NP_215893.1 Run
No TFOE experiment results were found

Quantitative Proteomics Data

t-test p-value Cholesterol/Glycerol Ratio
0.570000 1.43

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: