Rv1381 Dihydroorotase (EC 3.5.2.3)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv1381 pyrC Dihydroorotase (EC 3.5.2.3) CDS 1554188 1555480 + 1 293 430 FALSE

Rv1381 (Dihydroorotase (EC 3.5.2.3)) is predicted to be co-regulated in modules bicluster_0104 with residual 0.59 and bicluster_0137 with residual 0.49.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 3,600.00 and 3,500.00 for bicluster_0104 and 850.00 and 1,200.00 for bicluster_0137 respectively.

These modules are enriched for following go terms: pyrimidine nucleobase biosynthetic proce..., pyrimidine nucleobase metabolic process, nucleobase biosynthetic process 'de novo' pyrimidine nucleobase biosynth..., pyrimidine-containing compound biosynthe..., pyrimidine-containing compound metabolic..., organonitrogen compound metabolic proces..., nucleobase-containing small molecule met..., carbon-nitrogen ligase activity, with gl....

This gene is found to be for growth on cholesterol.

Mutant available?:

Product (LegacyBRC) Product (RefSeq)
Dihydroorotase dihydroorotase
Operon # Operon
928 - - - - - -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Dihydroorotase Pyrimidine metabolism
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Pyrimidine metabolism

41
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15608521 NP_215897.1 Run
GO:0004151

dihydroorotase activity

dihydroorotase activity

Details: 
Catalysis of the reaction: (S)-dihydroorotate + H(2)O = N-carbamoyl-L-aspartate + H(+).
GO Category: 
molecular_function
1
Total items in this category:  
GO:0040007

growth

growth

Details: 
The increase in size or mass of an entire organism, a part of an organism or a cell.
GO Category: 
biological_process
621
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
NA NA

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: