Rv1408 Ribulose-phosphate 3-epimerase (EC 5.1.3.1)

Summary

Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv1408 rpe Ribulose-phosphate 3-epimerase (EC 5.1.3.1) CDS 1584499 1585197 + 699 232 FALSE

Rv1408 (Ribulose-phosphate 3-epimerase (EC 5.1.3.1)) is predicted to be co-regulated in modules bicluster_0004 with residual 0.43 and bicluster_0564 with residual 0.54.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 5,500.00 and 6,900.00 for bicluster_0004 and 0.55 and 7.40 for bicluster_0564 respectively.

These modules are enriched for following go terms: .

This gene is found to be for growth on cholesterol.

Mutant available?:

BASS Score Distance to Tuberculist Start Codon Internal TSS Re-Annotated Start Tuberculist Annotated Start
-0.534 9 1584508 1584508 1584499
Product (LegacyBRC) Product (RefSeq)
Ribulose-phosphate 3-epimerase ribulose-phosphate 3-epimerase
Operon # Operon
944 - - -
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Pentose phosphate pathway

19
Total items in this category:  

KEGG

Pentose and glucuronate interconversions

8
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  

KEGG

Biosynthesis of secondary metabolites

326
Total items in this category:  

KEGG

Microbial metabolism in diverse environments

296
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15608546 NP_215924.1 Run
GO:0004750

ribulose-phosphate 3-epimerase activity

ribulose-phosphate 3-epimerase activity

Details: 
Catalysis of the reaction: D-ribulose 5-phosphate = D-xylulose 5-phosphate.
GO Category: 
molecular_function
1
Total items in this category:  
No TFOE experiment results were found

Quantitative Proteomics Data

t-test p-value Cholesterol/Glycerol Ratio
NA NA

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: