Rv1464 Cysteine desulfurase (EC 2.8.1.7), SufS subfamily

Summary

Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv1464 csd Cysteine desulfurase (EC 2.8.1.7), SufS subfamily CDS 1651518 1652771 + 1 254 417 FALSE

Rv1464 (Cysteine desulfurase (EC 2.8.1.7), SufS subfamily) is predicted to be co-regulated in modules bicluster_0369 with residual 0.51 and bicluster_0370 with residual 0.49.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 5.10 and 230.00 for bicluster_0369 and 0.02 and 38.00 for bicluster_0370 respectively.

These modules are enriched for following go terms: lipid biosynthetic process, lipid metabolic process, transferase activity, methyltransferase activity .

This gene is found to be for growth on cholesterol.

Mutant available?:

Product (LegacyBRC) Product (RefSeq)
Probable cysteine desulfurase cysteine desulfurase
Operon # Operon
976 - - - - - -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Cysteine desulfurase Thiamine metabolism
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Selenocompound metabolism

40
Total items in this category:  

KEGG

Thiamine metabolism

9
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15608602 NP_215980.1 Run
GO:0031071

cysteine desulfurase activity

cysteine desulfurase activity

Details: 
Catalysis of the reaction: L-cysteine + [enzyme]-cysteine = L-alanine + [enzyme]-S-sulfanylcysteine.
GO Category: 
molecular_function
2
Total items in this category:  
GO:0005515

protein binding

protein binding

Details: 
Interacting selectively and non-covalently with any protein or protein complex (a complex of two or more proteins that may include other nonprotein molecules).
GO Category: 
molecular_function
135
Total items in this category:  
GO:0005886

plasma membrane

plasma membrane

Details: 
The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
cellular_component
1284
Total items in this category:  
GO:0040007

growth

growth

Details: 
The increase in size or mass of an entire organism, a part of an organism or a cell.
GO Category: 
biological_process
621
Total items in this category:  
No TFOE experiment results were found

Quantitative Proteomics Data

t-test p-value Cholesterol/Glycerol Ratio
NA NA

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: