Rv1862 Alcohol dehydrogenase (EC 1.1.1.1)

Summary

Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv1862 adhA Alcohol dehydrogenase (EC 1.1.1.1) CDS 2109544 2110584 + 1 041 346 FALSE

Rv1862 (Alcohol dehydrogenase (EC 1.1.1.1)) is predicted to be co-regulated in modules bicluster_0156 with residual 0.52 and bicluster_0289 with residual 0.53.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 180.00 and 890.00 for bicluster_0156 and 0.00 and 0.03 for bicluster_0289 respectively.

These modules are enriched for following go terms: oxidoreductase activity, acting on the C..., oxidoreductase activity, acting on CH-OH....

This gene is found to be for growth on cholesterol.

Mutant available?:

Product (LegacyBRC) Product (RefSeq)
Probable alcohol dehydrogenase adhA alcohol dehydrogenase AdhA
Operon # Operon
1219
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Glycolysis / Gluconeogenesis

35
Total items in this category:  

KEGG

Fatty acid metabolism

51
Total items in this category:  

KEGG

Tyrosine metabolism

41
Total items in this category:  

KEGG

Chloroalkane and chloroalkene degradation

13
Total items in this category:  

KEGG

Naphthalene degradation

40
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  

KEGG

Biosynthesis of secondary metabolites

326
Total items in this category:  

KEGG

Microbial metabolism in diverse environments

296
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15608999 NP_216378.1 Run
No TFOE experiment results were found

Quantitative Proteomics Data

t-test p-value Cholesterol/Glycerol Ratio
0.030000 1.17

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: