Rv1877 EmrB/QacA family drug resistance transporter

Summary

Product Feature Type Start End Strand Length AA Length is TF
Rv1877 EmrB/QacA family drug resistance transporter CDS 2125904 2127967 + 2 064 687 FALSE

Rv1877 (EmrB/QacA family drug resistance transporter) is predicted to be co-regulated in modules bicluster_0398 with residual 0.53 and bicluster_0468 with residual 0.58.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 3.30 and 23.00 for bicluster_0398 and 14.00 and 16.00 for bicluster_0468 respectively.

These modules are enriched for following go terms: ligase activity, forming carbon-nitrogen... .

This gene is found to be for growth on cholesterol.

Mutant available?: Yes

Last update: 10/16/2017 - 11:45
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-17962 MT1926 1015
Product (LegacyBRC) Product (RefSeq)
PROBABLE CONSERVED INTEGRAL MEMBRANE PROTEIN integral membrane protein
Operon # Operon
1230
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15609014 NP_216393.1 Run
No TFOE experiment results were found

Quantitative Proteomics Data

t-test p-value Cholesterol/Glycerol Ratio
0.040000 0.96

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: