Rv2095c FIG019733: possible DNA-binding protein

Summary

Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv2095c pafC FIG019733: possible DNA-binding protein CDS 2353365 2354315 - 951 316 FALSE

Rv2095c (FIG019733: possible DNA-binding protein) is predicted to be co-regulated in modules bicluster_0192 with residual 0.54 and bicluster_0315 with residual 0.53.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 4,500.00 and 12,000.00 for bicluster_0192 and 0.00 and 0.00 for bicluster_0315 respectively.

These modules are enriched for following go terms: cellular macromolecular complex assembly, cellular protein complex assembly, protein complex assembly, macromolecular complex assembly, protein complex biogenesis .

This gene is found to be for growth on cholesterol.

Mutant available?: Yes

Last update: 10/16/2017 - 12:06
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-17938 MT2156 950
Product (LegacyBRC) Product (RefSeq)
Uncharacterized protein Rv2095c_MT2156
Operon # Operon
1372 - -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15609232 NP_216611.1 Run
GO:0005515

protein binding

protein binding

Details: 
Interacting selectively and non-covalently with any protein or protein complex (a complex of two or more proteins that may include other nonprotein molecules).
GO Category: 
molecular_function
135
Total items in this category:  
No TFOE experiment results were found

Quantitative Proteomics Data

t-test p-value Cholesterol/Glycerol Ratio
0.280000 1.39

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: