Rv2435c Adenylate cyclase (EC 4.6.1.1)

Summary

Product Feature Type Start End Strand Length AA Length is TF
Rv2435c Adenylate cyclase (EC 4.6.1.1) CDS 2730557 2732749 - 2 193 730 FALSE

Rv2435c (Adenylate cyclase (EC 4.6.1.1)) is predicted to be co-regulated in modules bicluster_0226 with residual 0.52 and bicluster_0275 with residual 0.52.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.00 and 2,000.00 for bicluster_0226 and 550.00 and 2,100.00 for bicluster_0275 respectively.

These modules are enriched for following go terms: .

This gene is found to be for growth on cholesterol.

Mutant available?: Yes

BASS Score Distance to Tuberculist Start Codon Internal TSS New Internal UTR Re-Annotated Start Tuberculist Annotated Start
-0.137 465 2732284 -393 2732677 2732749
Last update: 10/16/2017 - 13:07
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-15655 MT2509 1606
Product (LegacyBRC) Product (RefSeq)
PROBABLE CYCLASE [ADENYLYL-OR GUANYLYL-][ADENYLATE-OR GUANYLATE-] cyclase
Operon # Operon
1604 - - -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Adenylate cyclase Purine metabolism
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15609572 NP_216951.1 Run
GO:0004016

adenylate cyclase activity

adenylate cyclase activity

Details: 
Catalysis of the reaction: ATP = 3',5'-cyclic AMP + diphosphate.
GO Category: 
molecular_function
11
Total items in this category:  
No TFOE experiment results were found

Quantitative Proteomics Data

t-test p-value Cholesterol/Glycerol Ratio
0.580000 1.66

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: