Rv2678c Uroporphyrinogen III decarboxylase (EC 4.1.1.37)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv2678c hemE Uroporphyrinogen III decarboxylase (EC 4.1.1.37) CDS 2993989 2995062 - 1 074 357 FALSE

Rv2678c (Uroporphyrinogen III decarboxylase (EC 4.1.1.37)) is predicted to be co-regulated in modules bicluster_0236 with residual 0.53 and bicluster_0440 with residual 0.53.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 1,500.00 and 1,800.00 for bicluster_0236 and 0.01 and 67.00 for bicluster_0440 respectively.

These modules are enriched for following go terms: cellular macromolecule catabolic process, tetrapyrrole metabolic process, tetrapyrrole biosynthetic process, porphyrin-containing compound metabolic ..., porphyrin-containing compound biosynthet... .

This gene is found to be for growth on cholesterol.

Mutant available?:

Product (LegacyBRC) Product (RefSeq)
Uroporphyrinogen decarboxylase uroporphyrinogen decarboxylase
Operon # Operon
1755 - - -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Uroporphyrinogen decarboxylase Porphyrin and chlorophyll metabolism
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Porphyrin and chlorophyll metabolism

31
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  

KEGG

Biosynthesis of secondary metabolites

326
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15609815 NP_217194.1 Run
GO:0004853

uroporphyrinogen decarboxylase activity

uroporphyrinogen decarboxylase activity

Details: 
Catalysis of the reaction: uroporphyrinogen-III = coproporphyrinogen + 4 CO2.
GO Category: 
molecular_function
1
Total items in this category:  
GO:0005829

cytosol

cytosol

Details: 
The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
GO Category: 
cellular_component
371
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
NA NA

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: