Rv2713 Soluble pyridine nucleotide transhydrogenase (EC 1.6.1.1)

Summary

Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv2713 sthA Soluble pyridine nucleotide transhydrogenase (EC 1.6.1.1) CDS 3025441 3026847 + 1 407 468 FALSE

Rv2713 (Soluble pyridine nucleotide transhydrogenase (EC 1.6.1.1)) is predicted to be co-regulated in modules bicluster_0196 with residual 0.45 and bicluster_0524 with residual 0.54.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 120.00 and 3,000.00 for bicluster_0196 and 0.00 and 1.00 for bicluster_0524 respectively.

These modules are enriched for following go terms: .

This gene is found to be for growth on cholesterol.

Mutant available?: Yes

BASS Score Distance to Tuberculist Start Codon Internal TSS Primary TSS Re-Annotated Start Tuberculist Annotated Start
-0.404 1100 3026541 3025432 3025432 3025441
Last update: 10/16/2017 - 14:17
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-15690 MT2786 1593
Product (LegacyBRC) Product (RefSeq)
Probable soluble pyridine nucleotide transhydrogenase soluble pyridine nucleotide transhydrogenase
Operon # Operon
1782
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

NAD(P)(+) transhydrogenase (B-specific) Nicotinate and nicotinamide metabolism
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Nicotinate and nicotinamide metabolism

16
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15609850 NP_217229.1 Run
GO:0003957

NAD(P)+ transhydrogenase (B-specific) activity

NAD(P)+ transhydrogenase (B-specific) activity

Details: 
Catalysis of the reaction: NADPH + H+ + NAD+ = NADP+ + NADH + H+, driving the transfer of a solute or solutes from one side of a membrane to the other. In the course of the reaction (left to right) one H atom is transferred from inside the cell to outside. The reaction is B-specific (i.e. the pro-S hydrogen is transferred from the 4-position of reduced nicotinamide cofactor) with respect to both NAD+ and NADP+.
GO Category: 
molecular_function
1
Total items in this category:  
GO:0005829

cytosol

cytosol

Details: 
The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
GO Category: 
cellular_component
371
Total items in this category:  
GO:0005886

plasma membrane

plasma membrane

Details: 
The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
cellular_component
1284
Total items in this category:  
No TFOE experiment results were found

Quantitative Proteomics Data

t-test p-value Cholesterol/Glycerol Ratio
0.430000 1.41

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: