Rv2857c Short-chain dehydrogenase/reductase in hypothetical Actinobacterial gene cluster

Product Feature Type Start End Strand Length AA Length is TF
Rv2857c Short-chain dehydrogenase/reductase in hypothetical Actinobacterial gene cluster CDS 3168583 3169359 - 777 258 FALSE

Rv2857c (Short-chain dehydrogenase/reductase in hypothetical Actinobacterial gene cluster) is predicted to be co-regulated in modules bicluster_0083 with residual 0.52 and bicluster_0177 with residual 0.50.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 240.00 and 440.00 for bicluster_0083 and 3.10 and 110.00 for bicluster_0177 respectively.

These modules are enriched for following go terms: glutamine metabolic process glutamine metabolic process.

This gene is found to be for growth on cholesterol.

Mutant available?:

BASS Score Re-Annotated Start Tuberculist Annotated Start
-1.477 3169356 3169359
Product (LegacyBRC) Product (RefSeq)
Operon # Operon
1869 - - -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics


Not assigned Not assigned
Locus Tuberculist Genome View


Locus Tag KEGG Pathways


not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network


GI Number Protein ID Blast Conserved Domains
15609994 NP_217373.1 Run
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.420000 0.68

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: