Rv2861c Methionine aminopeptidase (EC 3.4.11.18)

Summary

Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv2861c mapB Methionine aminopeptidase (EC 3.4.11.18) CDS 3173160 3174017 - 858 285 FALSE

Rv2861c (Methionine aminopeptidase (EC 3.4.11.18)) is predicted to be co-regulated in modules bicluster_0054 with residual 0.52 and bicluster_0556 with residual 0.55.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.32 and 0.27 for bicluster_0054 and 0.02 and 1.40 for bicluster_0556 respectively.

These modules are enriched for following go terms: cobalamin binding.

This gene is found to be for growth on cholesterol.

Mutant available?:

Product (LegacyBRC) Product (RefSeq)
Methionine aminopeptidase methionine aminopeptidase
Operon # Operon
1870
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
57117031 YP_177911.1 Run
GO:0004239

methionyl aminopeptidase activity

methionyl aminopeptidase activity

Details: 
OBSOLETE. Catalysis of the release of N-terminal amino acids, preferentially methionine, from peptides and arylamides.
GO Category: 
molecular_function
2
Total items in this category:  
No TFOE experiment results were found

Quantitative Proteomics Data

t-test p-value Cholesterol/Glycerol Ratio
NA NA

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: