Rv2905 Putative lipoprotein lppW precursor

Summary

Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv2905 lppW Putative lipoprotein lppW precursor CDS 3214628 3215572 + 945 314 FALSE

Rv2905 (Putative lipoprotein lppW precursor) is predicted to be co-regulated in modules bicluster_0064 with residual 0.44 and bicluster_0227 with residual 0.50.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 500.00 and 2,500.00 for bicluster_0064 and 89.00 and 1,900.00 for bicluster_0227 respectively.

These modules are enriched for following go terms: protein secretion, secretion by cell, secretion, establishment of localization in cell, cellular localization, antibiotic metabolic process, drug metabolic process, protein transport, establishment of protein localization .

This gene is found to be for growth on cholesterol.

Mutant available?: Yes

Last update: 10/16/2017 - 14:41
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-14755 MT2973 77
Product (LegacyBRC) Product (RefSeq)
Putative lipoprotein lppW alanine rich lipoprotein LppW
Operon # Operon
1898
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15610042 NP_217421.1 Run
GO:0005576

extracellular region

extracellular region

Details: 
The space external to the outermost structure of a cell. For cells without external protective or external encapsulating structures this refers to space outside of the plasma membrane. This term covers the host cell environment outside an intracellular parasite.
GO Category: 
cellular_component
272
Total items in this category:  
No TFOE experiment results were found

Quantitative Proteomics Data

t-test p-value Cholesterol/Glycerol Ratio
0.900000 2.56

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: