Rv2951c Phthiodiolone/phenolphthiodiolone dimycocerosates ketoreductase

Summary
Product Feature Type Start End Strand Length AA Length is TF
Rv2951c Phthiodiolone/phenolphthiodiolone dimycocerosates ketoreductase CDS 3303103 3304248 - 1 146 381 FALSE

Rv2951c (Phthiodiolone/phenolphthiodiolone dimycocerosates ketoreductase) is predicted to be co-regulated in modules bicluster_0255 with residual 0.52 and bicluster_0396 with residual 0.50.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.00 and 3.80 for bicluster_0255 and 0.01 and 9.30 for bicluster_0396 respectively.

These modules are enriched for following go terms: organic hydroxy compound transport, tetracycline transport, antibiotic transport, toxin transport, drug transport, response to drug, tetracycline transporter activity, drug:hydrogen antiporter activity, tetracycline:hydrogen antiporter activit..., toxin transporter activity, antibiotic transporter activity, organic hydroxy compound transmembrane t..., solute:cation antiporter activity, solute:hydrogen antiporter activity, antiporter activity.

This gene is found to be for growth on cholesterol.

Mutant available?: Yes

Last update: 10/16/2017 - 14:41
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-13642 MT3025 991
Product (LegacyBRC) Product (RefSeq)
Phthiodiolone_phenolphthiodiolone dimycocerosates ketoreductase oxidoreductase
Operon # Operon
1922
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

DDT degradation

2
Total items in this category:  

KEGG

Limonene and pinene degradation

65
Total items in this category:  

KEGG

Biosynthesis of secondary metabolites

326
Total items in this category:  

KEGG

Microbial metabolism in diverse environments

296
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15610088 NP_217467.1 Run
GO:0005829

cytosol

cytosol

Details: 
The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
GO Category: 
cellular_component
371
Total items in this category:  
GO:0005886

plasma membrane

plasma membrane

Details: 
The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
cellular_component
1284
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.500000 1.49

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: