Rv3229c POSSIBLE LINOLEOYL-CoA DESATURASE (DELTA(6)-DESATURASE)

Summary

Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv3229c desA3 POSSIBLE LINOLEOYL-CoA DESATURASE (DELTA(6)-DESATURASE) CDS 3605751 3607034 - 1 284 427 FALSE

Rv3229c (POSSIBLE LINOLEOYL-CoA DESATURASE (DELTA(6)-DESATURASE)) is predicted to be co-regulated in modules bicluster_0202 with residual 0.55 and bicluster_0374 with residual 0.50.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.01 and 150.00 for bicluster_0202 and 0.01 and 3,200.00 for bicluster_0374 respectively.

These modules are enriched for following go terms: .

This gene is found to be for growth on cholesterol.

Mutant available?: Yes

Last update: 10/16/2017 - 15:27
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-17875 MT3326 433
Product (LegacyBRC) Product (RefSeq)
POSSIBLE LINOLEOYL-CoA DESATURASE [DELTA[6]-DESATURASE] linoleoyl-CoA desaturase
Operon # Operon
2114
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Linoleic acid metabolism

3
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
57117078 YP_177948.1 Run
GO:0005515

protein binding

protein binding

Details: 
Interacting selectively and non-covalently with any protein or protein complex (a complex of two or more proteins that may include other nonprotein molecules).
GO Category: 
molecular_function
135
Total items in this category:  
GO:0005886

plasma membrane

plasma membrane

Details: 
The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
cellular_component
1284
Total items in this category:  
GO:0016213

linoleoyl-CoA desaturase activity

linoleoyl-CoA desaturase activity

Details: 
Catalysis of the reaction: linoleoyl-CoA + reduced acceptor + O2 = gamma-linolenoyl-CoA + acceptor + 2 H2O.
GO Category: 
molecular_function
1
Total items in this category:  
No TFOE experiment results were found

Quantitative Proteomics Data

t-test p-value Cholesterol/Glycerol Ratio
0.180000 0.04

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: