Rv3565 Valine--pyruvate aminotransferase (EC 2.6.1.66)

Summary

Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv3565 aspB Valine--pyruvate aminotransferase (EC 2.6.1.66) CDS 4006200 4007366 + 1 167 388 FALSE

Rv3565 (Valine--pyruvate aminotransferase (EC 2.6.1.66)) is predicted to be co-regulated in modules bicluster_0037 with residual 0.37 and bicluster_0152 with residual 0.50.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.00 and 0.00 for bicluster_0037 and 0.00 and 0.09 for bicluster_0152 respectively.

These modules are enriched for following go terms: cellular macromolecule metabolic process, cellular nitrogen compound metabolic pro..., cellular aromatic compound metabolic pro..., heterocycle metabolic process, organic cyclic compound metabolic proces..., macromolecule metabolic process, heterocyclic compound binding, organic cyclic compound binding .

This gene is found to be for growth on cholesterol.

Mutant available?: Yes

Last update: 10/16/2017 - 15:33
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-14947 MT3670 340
Product (LegacyBRC) Product (RefSeq)
POSSIBLE ASPARTATE AMINOTRANSFERASE ASPB [TRANSAMINASE A] [ASPAT] [GLUTAMIC--OXALOACETIC TRANSAMINASE] [GLUTAMIC--ASPARTIC TRANSAMINASE] aspartate aminotransferase
Operon # Operon
2323 - - - -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Valine--pyruvate transaminase Valine, leucine and isoleucine biosynthesis
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15610701 NP_218082.1 Run
No TFOE experiment results were found

Quantitative Proteomics Data

t-test p-value Cholesterol/Glycerol Ratio
0.350000 1.54

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: