Rv3593 Beta-lactamase (EC 3.5.2.6)

Summary

Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv3593 lpqF Beta-lactamase (EC 3.5.2.6) CDS 4034352 4035710 + 1 359 452 FALSE

Rv3593 (Beta-lactamase (EC 3.5.2.6)) is predicted to be co-regulated in modules bicluster_0145 with residual 0.56 and bicluster_0252 with residual 0.55.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.00 and 0.16 for bicluster_0145 and 930.00 and 3,100.00 for bicluster_0252 respectively.

These modules are enriched for following go terms: riboflavin metabolic process, riboflavin biosynthetic process, flavin-containing compound metabolic pro..., flavin-containing compound biosynthetic ... regulation of cell shape, regulation of anatomical structure morph..., regulation of cell morphogenesis, regulation of developmental process, cell morphogenesis, anatomical structure morphogenesis, developmental process, cellular component morphogenesis, single-organism developmental process, anatomical structure development, cellular developmental process, regulation of cellular component organiz..., cellular component organization.

This gene is found to be for growth on cholesterol.

Mutant available?:

BASS Score New Primary UTR Primary TSS Re-Annotated Start Tuberculist Annotated Start
-0.7 162 4034232 4034394 4034352
Product (LegacyBRC) Product (RefSeq)
PROBABLE CONSERVED LIPOPROTEIN LPQF lipoprotein LpqF
Operon # Operon
2341 -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Beta-lactamase beta-Lactam resistance, Penicillin and cephalosporin biosynthesis
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15610729 NP_218110.1 Run
GO:0040007

growth

growth

Details: 
The increase in size or mass of an entire organism, a part of an organism or a cell.
GO Category: 
biological_process
621
Total items in this category:  
No TFOE experiment results were found

Quantitative Proteomics Data

t-test p-value Cholesterol/Glycerol Ratio
NA NA

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: