Rv3601c Aspartate 1-decarboxylase (EC 4.1.1.11)

Summary

Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv3601c panD Aspartate 1-decarboxylase (EC 4.1.1.11) CDS 4043862 4044281 - 420 139 FALSE

Rv3601c (Aspartate 1-decarboxylase (EC 4.1.1.11)) is predicted to be co-regulated in modules bicluster_0370 with residual 0.49 and bicluster_0556 with residual 0.55.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.02 and 38.00 for bicluster_0370 and 0.02 and 1.40 for bicluster_0556 respectively.

These modules are enriched for following go terms: cobalamin binding.

This gene is found to be for growth on cholesterol.

Mutant available?:

Product (LegacyBRC) Product (RefSeq)
Aspartate 1-decarboxylase aspartate alpha-decarboxylase
Operon # Operon
2346 - - - - -
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

beta-Alanine metabolism

37
Total items in this category:  

KEGG

Pantothenate and CoA biosynthesis

18
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  

KEGG

Biosynthesis of secondary metabolites

326
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15610737 NP_218118.1 Run
GO:0004068

aspartate 1-decarboxylase activity

aspartate 1-decarboxylase activity

Details: 
Catalysis of the reaction: L-aspartate = beta-alanine + CO2.
GO Category: 
molecular_function
1
Total items in this category:  
GO:0004068

aspartate 1-decarboxylase activity

aspartate 1-decarboxylase activity

Details: 
Catalysis of the reaction: L-aspartate = beta-alanine + CO2.
GO Category: 
molecular_function
1
Total items in this category:  
GO:0005618

cell wall

cell wall

Details: 
The rigid or semi-rigid envelope lying outside the cell membrane of plant, fungal, and most prokaryotic cells, maintaining their shape and protecting them from osmotic lysis. In plants it is made of cellulose and, often, lignin; in fungi it is composed largely of polysaccharides; in bacteria it is composed of peptidoglycan.
GO Category: 
cellular_component
320
Total items in this category:  
GO:0005829

cytosol

cytosol

Details: 
The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
GO Category: 
cellular_component
371
Total items in this category:  
GO:0051289

protein homotetramerization

protein homotetramerization

Details: 
The formation of a protein homotetramer, a macromolecular structure consisting of four noncovalently associated identical subunits.
GO Category: 
biological_process
17
Total items in this category:  
No TFOE experiment results were found

Quantitative Proteomics Data

t-test p-value Cholesterol/Glycerol Ratio
NA NA

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: