Rv3684 Cysteine synthase (EC 2.5.1.47)

Summary

Product Feature Type Start End Strand Length AA Length is TF
Rv3684 Cysteine synthase (EC 2.5.1.47) CDS 4125439 4126479 + 1 041 346 FALSE

Rv3684 (Cysteine synthase (EC 2.5.1.47)) is predicted to be co-regulated in modules bicluster_0234 with residual 0.50 and bicluster_0251 with residual 0.46.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 44.00 and 9,500.00 for bicluster_0234 and 780.00 and 7,200.00 for bicluster_0251 respectively.

These modules are enriched for following go terms: cysteine metabolic process, L-serine metabolic process, serine family amino acid biosynthetic pr..., sulfur amino acid biosynthetic process, small molecule biosynthetic process, single-organism biosynthetic process, sulfur amino acid metabolic process, alpha-amino acid biosynthetic process.

This gene is found to be for growth on cholesterol.

Mutant available?:

BASS Score Re-Annotated Start Tuberculist Annotated Start
-0.788 4125373 4125439
Product (LegacyBRC) Product (RefSeq)
PROBABLE LYASE lyase
Operon # Operon
2400
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Cysteine synthase Cysteine and methionine metabolism, Sulfur metabolism
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Cysteine and methionine metabolism

28
Total items in this category:  

KEGG

Selenocompound metabolism

40
Total items in this category:  

KEGG

Sulfur metabolism

12
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  

KEGG

Microbial metabolism in diverse environments

296
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15610820 NP_218201.1 Run
GO:0005829

cytosol

cytosol

Details: 
The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
GO Category: 
cellular_component
371
Total items in this category:  
GO:0005886

plasma membrane

plasma membrane

Details: 
The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
cellular_component
1284
Total items in this category:  
No TFOE experiment results were found

Quantitative Proteomics Data

t-test p-value Cholesterol/Glycerol Ratio
0.760000 1.37

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: