Rv3730c ATP-dependent DNA ligase (EC 6.5.1.1)

Summary

Product Feature Type Start End Strand Length AA Length is TF
Rv3730c ATP-dependent DNA ligase (EC 6.5.1.1) CDS 4180680 4181720 - 1 041 346 FALSE

Rv3730c (ATP-dependent DNA ligase (EC 6.5.1.1)) is predicted to be co-regulated in modules bicluster_0139 with residual 0.43 and bicluster_0427 with residual 0.47.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 1,500.00 and 6,300.00 for bicluster_0139 and 0.00 and 4.60 for bicluster_0427 respectively.

These modules are enriched for following go terms: galactose metabolic process, oxidoreductase activity, acting on the C....

This gene is found to be for growth on cholesterol.

Mutant available?:

Product (LegacyBRC) Product (RefSeq)
Putative uncharacterized protein
Operon # Operon
2435
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Base excision repair

17
Total items in this category:  

KEGG

Nucleotide excision repair

14
Total items in this category:  

KEGG

Mismatch repair

19
Total items in this category:  

KEGG

Non-homologous end-joining

5
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15610866 NP_218247.1 Run
GO:0003910

DNA ligase (ATP) activity

DNA ligase (ATP) activity

Details: 
Catalysis of the reaction: ATP + deoxyribonucleotide(n) + deoxyribonucleotide(m) = AMP + diphosphate + deoxyribonucleotide(n+m).
GO Category: 
molecular_function
3
Total items in this category:  
No TFOE experiment results were found

Quantitative Proteomics Data

t-test p-value Cholesterol/Glycerol Ratio
0.660000 2.75

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: