Rv3762c alkyl sulfatase (EC 3.1.6.-)

Summary

Product Feature Type Start End Strand Length AA Length is TF
Rv3762c alkyl sulfatase (EC 3.1.6.-) CDS 4206996 4208876 - 1 881 626 FALSE

Rv3762c (alkyl sulfatase (EC 3.1.6.-)) is predicted to be co-regulated in modules bicluster_0122 with residual 0.57 and bicluster_0372 with residual 0.47.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 1.10 and 6.50 for bicluster_0122 and 360.00 and 1,500.00 for bicluster_0372 respectively.

These modules are enriched for following go terms: cellular protein modification process, protein modification process, phosphorylation, protein kinase activity .

This gene is found to be for growth on cholesterol.

Mutant available?: Yes

BASS Score Distance to Tuberculist Start Codon Internal TSS Re-Annotated Start Tuberculist Annotated Start
-0.116 6 4208870 4208870 4208876
Last update: 10/16/2017 - 16:15
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-13638 MT3869 99
Product (LegacyBRC) Product (RefSeq)
POSSIBLE HYDROLASE hydrolase
Operon # Operon
2458
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15610898 NP_218279.1 Run
GO:0004065

arylsulfatase activity

arylsulfatase activity

Details: 
Catalysis of the reaction: a phenol sulfate + H2O = a phenol + sulfate.
GO Category: 
molecular_function
3
Total items in this category:  
No TFOE experiment results were found

Quantitative Proteomics Data

t-test p-value Cholesterol/Glycerol Ratio
0.010000 1.35

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: