Organism : Pseudomonas aeruginosa | Module List :
PA0164

probable gamma-glutamyltranspeptidase (NCBI)

CircVis
Functional Annotations (8)
Function System
Gamma-glutamyltransferase cog/ cog
gamma-glutamyltransferase activity go/ molecular_function
Taurine and hypotaurine metabolism kegg/ kegg pathway
Selenocompound metabolism kegg/ kegg pathway
Cyanoamino acid metabolism kegg/ kegg pathway
Glutathione metabolism kegg/ kegg pathway
Arachidonic acid metabolism kegg/ kegg pathway
Metabolic pathways kegg/ kegg pathway
GeneModule member RegulatorRegulator MotifMotif

Cytoscape Web
Regulation information for PA0164
(Mouseover regulator name to see its description)

PA0164 is regulated by 36 influences and regulates 0 modules.
Regulators for PA0164 (36)
Regulator Module Operator
PA0032 266 tf
PA0163 266 tf
PA0253 266 tf
PA1359 266 tf
PA2050 266 tf
PA2766 266 tf
PA3002 266 tf
PA3133 266 tf
PA3364 266 tf
PA3423 266 tf
PA4354 266 tf
PA4363 266 tf
PA4989 266 tf
PA5085 266 tf
PA5253 266 tf
PA5342 266 tf
PA5431 266 tf
PA0207 240 tf
PA0376 240 tf
PA0527 240 tf
PA0791 240 tf
PA0815 240 tf
PA1067 240 tf
PA1125 240 tf
PA2056 240 tf
PA2118 240 tf
PA2899 240 tf
PA3594 240 tf
PA4132 240 tf
PA4169 240 tf
PA4196 240 tf
PA4890 240 tf
PA4989 240 tf
PA5293 240 tf
PA5437 240 tf
PA5562 240 tf

Warning: PA0164 Does not regulate any modules!

Motif information (de novo identified motifs for modules)

There are 4 motifs predicted.

Motif Table (4)
Motif Id e-value Consensus Motif Logo
3308 1.50e-02 aaaTttTt.t.taCa.c.atgTgA
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3309 3.40e+02 gCAaccatAaCaaCc.ggA
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3360 3.60e-19 aa.AaAGat.g.TAaCgAttgt
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3361 3.60e-07 AcCtGCGGatg.aCgagggAcCCg
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Motif Help

Transcription factor binding motifs help to elucidate regulatory mechanism. cMonkey integrates powerful de novo motif detection to identify conditionally co-regulated sets of genes. De novo predicted motifs for each module are listed in the module page as motif logo images along with associated prediction statistics (e-values). The main module page also shows the location of these motifs within the upstream sequences of the module member genes.

Motifs of interest can be broadcasted to RegPredict (currently only available for Desulfovibrio vulgaris Hildenborough) in order to compare conservation in similar species. This integrated motif prediction and comparative analysis provides an additional checkpoint for regulatory motif prediction confidence.

Motif e-value: cMonkey tries to identify two motifs per modules in the upstream sequences of the module member genes. Motif e-value is an indicative of the motif co-occurences between the members of the module.Smaller e-values are indicative of significant sequence motifs. Our experience showed that e-values smaller than 10 are generally indicative of significant motifs.

Functional Enrichment for PA0164

PA0164 is enriched for 8 functions in 3 categories.
Enrichment Table (8)
Function System
Gamma-glutamyltransferase cog/ cog
gamma-glutamyltransferase activity go/ molecular_function
Taurine and hypotaurine metabolism kegg/ kegg pathway
Selenocompound metabolism kegg/ kegg pathway
Cyanoamino acid metabolism kegg/ kegg pathway
Glutathione metabolism kegg/ kegg pathway
Arachidonic acid metabolism kegg/ kegg pathway
Metabolic pathways kegg/ kegg pathway
Module neighborhood information for PA0164

PA0164 has total of 49 gene neighbors in modules 240, 266
Gene neighbors (49)
Gene Common Name Description Module membership
PA0025 aroE shikimate dehydrogenase (NCBI) 240, 333
PA0164 PA0164 probable gamma-glutamyltranspeptidase (NCBI) 240, 266
PA0239 PA0239 hypothetical protein (NCBI) 266, 536
PA0749 PA0749 hypothetical protein (NCBI) 68, 266
PA0989 PA0989 hypothetical protein (NCBI) 266, 323
PA1051 PA1051 probable transporter (NCBI) 240, 337
PA1052 PA1052 hypothetical protein (NCBI) 240, 337
PA1309 PA1309 probable transcriptional regulator (NCBI) 266, 438
PA1416 PA1416 hypothetical protein (NCBI) 151, 240
PA1417 PA1417 probable decarboxylase (NCBI) 151, 240
PA1418 PA1418 probable sodium:solute symport protein (NCBI) 151, 240
PA1419 PA1419 probable transporter (NCBI) 151, 240
PA1420 PA1420 hypothetical protein (NCBI) 151, 240
PA1421 gbuA guanidinobutyrase (NCBI) 151, 240
PA1450 PA1450 hypothetical protein (NCBI) 266, 344
PA1466 PA1466 hypothetical protein (NCBI) 266, 507
PA1600 PA1600 probable cytochrome c (NCBI) 240, 546
PA1601 PA1601 probable aldehyde dehydrogenase (NCBI) 240, 546
PA1602 PA1602 probable oxidoreductase (NCBI) 240, 546
PA1734 PA1734 hypothetical protein (NCBI) 178, 266
PA1735 PA1735 hypothetical protein (NCBI) 178, 266
PA2757 PA2757 hypothetical protein (NCBI) 240, 247
PA2766 PA2766 probable transcriptional regulator (NCBI) 266, 323
PA2767 PA2767 probable enoyl-CoA hydratase/isomerase (NCBI) 266, 323
PA2768 PA2768 hypothetical protein (NCBI) 266, 323
PA3018 PA3018 hypothetical protein (NCBI) 240, 333
PA3035 PA3035 probable glutathione S-transferase (NCBI) 240, 377
PA3276 PA3276 hypothetical protein (NCBI) 255, 266
PA3281 PA3281 hypothetical protein (NCBI) 48, 240
PA3282 PA3282 hypothetical protein (NCBI) 48, 240
PA3283 PA3283 hypothetical protein (NCBI) 48, 240
PA3284 PA3284 hypothetical protein (NCBI) 48, 240
PA3825 PA3825 hypothetical protein (NCBI) 245, 266
PA3826 PA3826 hypothetical protein (NCBI) 266, 375
PA4167 PA4167 probable oxidoreductase (NCBI) 175, 266
PA4770 lldP L-lactate permease (NCBI) 82, 240
PA4771 lldD L-lactate dehydrogenase (NCBI) 82, 240
PA4772 PA4772 probable ferredoxin (NCBI) 82, 240
PA4891 ureE urease accessory protein UreE (NCBI) 240, 380
PA4892 ureF urease accessory protein UreF (NCBI) 44, 240
PA4988 waaA 3-deoxy-D-manno-octulosonic-acid transferase (NCBI) 266, 422
PA4989 PA4989 probable transcriptional regulator (NCBI) 266, 335
PA4990 PA4990 SMR multidrug efflux transporter (NCBI) 23, 266
PA4991 PA4991 hypothetical protein (NCBI) 58, 266
PA4992 PA4992 hypothetical protein (NCBI) 253, 266
PA5085 PA5085 probable transcriptional regulator (NCBI) 220, 266
PA5311 PA5311 probable major facilitator superfamily (MFS) transporter (NCBI) 266, 335
PA5440 PA5440 probable peptidase (NCBI) 240, 333
PA5442 PA5442 hypothetical protein (NCBI) 266, 438
Gene Page Help

Network Tab

If the gene is associated with a module(s), its connection to given modules along with other members of that module are shown as network by using CytoscapeWeb. In this view, each green colored circular nodes represent module member genes, purple colored diamonds represent module motifs and red triangles represent regulators. Each node is connected to module (Bicluster) via edges. This representation provides quick overview of all genes, regulators and motifs for modules. It also allows one to see shared genes/motifs/regulators among diferent modules.

Network representation is interactive. You can zoom in/out and move nodes/edges around. Clicking on a node will open up a window to give more details. For genes, Locus tag, organism, genomic coordinates, NCBI gene ID, whether it is transcription factor or not and any associated functional information will be shown. For regulators, number of modules are shown in addition to gene details. For motifs, e-value, consensus sequence and sequence logo will be shown. For modules, expression profile plot, motif information, functional associations and motif locations for each member of the module will be shown.
You can pin information boxes by using button in the box title and open up additional ones on the same screen for comparative analysis.

Regulation Tab

Regulation tab for each gene includes regulatory influences such as environmental factors or transcription factors or their combinations identified by regulatory network inference algorithms.

If the gene is a member of a module, regulators influencing that module are also considered to regulate the gene. Regulators table list total number of regulatory influences, regulators, modules and type of the influence.

You can see description of the regulator inside the tooltip when you mouseover. In certain cases the regulatory influence is predicted to be the result of the combination of two influences. These are indicated as combiner in the column labeled "Operator".

For transcription factors, an additional table next to regulator table will be show. This table show modules that are influenced by the transcription factor.

Motifs Tab

Network inference algorithm uses de novo motif prediction for assigning genes to modules. If there are any motifs identified in the upstream region of a gene, the motif will be shown here. For each motif sequence logo, consensus and e-value will be shown.

Functions Tab

Identification of functional enrichment for the module members is important in associating predicted motifs and regulatory influences with pathways. As described above, the network inference pipeline includes a functional enrichment module by which hypergeometric p-values are used to identify over representation of functional ontology terms among module members.

Network Portal presents functional ontologies from KEGG, GO, TIGRFAM, and COG as separate tables that include function name, type, corrected and uncorrected hypergeometric p-values, and the number of genes assigned to this category out of total number of genes in the module.

Module Members Tab

Identity of gene members in a module may help to identify potential interactions between different functional modules. Therefore, neighbor genes that share the same module(s) with gene under consideration are shown here. For each memebr, gene name, description and modules that contain it are listed.

Help Tab

This help page. More general help can be accessed by clicking help menu in the main navigation bar.

Social Tab

Network Portal is designed to promote collaboration through social interactions. Therefore interested researchers can share information, questions and updates for a particular gene.

Users can use their Disqus, Facebook, Twitter or Google accounts to connect to this page (We recommend Google). Each module and gene page includes comments tab that lists history of the interactions for that gene. You can browse the history, make updates, raise questions and share these activities with social web.

In the next releases of the network portal, we are planning to create personal space for each user where you can share you space that contains all the analysis steps you did along with relevant information.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend
Comments for PA0164
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Gene Help

Overview

Gene landing pages present genomic, functional, and regulatory information for individual genes. A circular visualization displays connections between the selected gene and genes in the same modules, with as edges drawn between the respective coordinates of the whole genome.

The gene page also lists functional ontology assignments, module membership, and motifs associated with these modules. Genes in the network inherit regulatory influences from the modules to which they belong. Therefore, the regulatory information for each gene is a collection of all regulatory influences on these modules. These are listed as a table that includes influence name, type, and target module. If the gene is a transcription factor, its target modules are also displayed in a table that provides residual values and number of genes.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend