Organism : Pseudomonas aeruginosa | Module List :
PA1843 metH

methionine synthase (NCBI)

CircVis
Functional Annotations (14)
Function System
Methionine synthase I, cobalamin-binding domain cog/ cog
dihydropteroate synthase activity go/ molecular_function
intracellular go/ cellular_component
methionine synthase activity go/ molecular_function
homocysteine S-methyltransferase activity go/ molecular_function
methionine biosynthetic process go/ biological_process
folic acid-containing compound biosynthetic process go/ biological_process
cobalamin binding go/ molecular_function
cobalt ion binding go/ molecular_function
Cysteine and methionine metabolism kegg/ kegg pathway
One carbon pool by folate kegg/ kegg pathway
Metabolic pathways kegg/ kegg pathway
Biosynthesis of secondary metabolites kegg/ kegg pathway
metH tigr/ tigrfam
GeneModule member RegulatorRegulator MotifMotif

Cytoscape Web
Regulation information for PA1843
(Mouseover regulator name to see its description)

PA1843 is regulated by 32 influences and regulates 0 modules.
Regulators for PA1843 metH (32)
Regulator Module Operator
PA0179 14 tf
PA0547 14 tf
PA0893 14 tf
PA1898 14 tf
PA2692 14 tf
PA3002 14 tf
PA3604 14 tf
PA3804 14 tf
PA4269 14 tf
PA4275 14 tf
PA4279 14 tf
PA4530 14 tf
PA5239 14 tf
PA5389 14 tf
PA5403 14 tf
PA5550 14 tf
PA5562 14 tf
PA0393 94 tf
PA0547 94 tf
PA1526 94 tf
PA1776 94 tf
PA3002 94 tf
PA3604 94 tf
PA3804 94 tf
PA3948 94 tf
PA4052 94 tf
PA4451 94 tf
PA4530 94 tf
PA4547 94 tf
PA4755 94 tf
PA5344 94 tf
PA5562 94 tf

Warning: PA1843 Does not regulate any modules!

Motif information (de novo identified motifs for modules)

There are 4 motifs predicted.

Motif Table (4)
Motif Id e-value Consensus Motif Logo
2864 4.10e-01 gaTTtttcCGg
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2865 2.30e+01 CtgCaaT.TAGcatattgccC
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3020 1.60e+03 TTT.CcccT
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3021 1.60e+04 TTC.gAGTTT
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Motif Help

Transcription factor binding motifs help to elucidate regulatory mechanism. cMonkey integrates powerful de novo motif detection to identify conditionally co-regulated sets of genes. De novo predicted motifs for each module are listed in the module page as motif logo images along with associated prediction statistics (e-values). The main module page also shows the location of these motifs within the upstream sequences of the module member genes.

Motifs of interest can be broadcasted to RegPredict (currently only available for Desulfovibrio vulgaris Hildenborough) in order to compare conservation in similar species. This integrated motif prediction and comparative analysis provides an additional checkpoint for regulatory motif prediction confidence.

Motif e-value: cMonkey tries to identify two motifs per modules in the upstream sequences of the module member genes. Motif e-value is an indicative of the motif co-occurences between the members of the module.Smaller e-values are indicative of significant sequence motifs. Our experience showed that e-values smaller than 10 are generally indicative of significant motifs.

Functional Enrichment for PA1843

PA1843 is enriched for 14 functions in 3 categories.
Enrichment Table (14)
Function System
Methionine synthase I, cobalamin-binding domain cog/ cog
dihydropteroate synthase activity go/ molecular_function
intracellular go/ cellular_component
methionine synthase activity go/ molecular_function
homocysteine S-methyltransferase activity go/ molecular_function
methionine biosynthetic process go/ biological_process
folic acid-containing compound biosynthetic process go/ biological_process
cobalamin binding go/ molecular_function
cobalt ion binding go/ molecular_function
Cysteine and methionine metabolism kegg/ kegg pathway
One carbon pool by folate kegg/ kegg pathway
Metabolic pathways kegg/ kegg pathway
Biosynthesis of secondary metabolites kegg/ kegg pathway
metH tigr/ tigrfam
Module neighborhood information for PA1843

PA1843 has total of 30 gene neighbors in modules 14, 94
Gene neighbors (30)
Gene Common Name Description Module membership
PA0556 PA0556 hypothetical protein (NCBI) 14, 253
PA0761 nadB L-aspartate oxidase (NCBI) 94, 165
PA0903 alaS alanyl-tRNA synthetase (NCBI) 14, 260
PA1031 PA1031 hypothetical protein (NCBI) 94, 384
PA1529 lig DNA ligase (NCBI) 14, 396
PA1532 dnaX DNA polymerase III subunits gamma and tau (NCBI) 94, 178
PA1842 PA1842 hypothetical protein (NCBI) 14, 94
PA1843 metH methionine synthase (NCBI) 14, 94
PA2613 PA2613 hypothetical protein (NCBI) 94, 390
PA2615 ftsK cell division protein FtsK (NCBI) 94, 296
PA2978 ptpA phosphotyrosine protein phosphatase (NCBI) 1, 14
PA2981 lpxK tetraacyldisaccharide 4'-kinase (NCBI) 1, 14
PA3070 PA3070 hypothetical protein (NCBI) 60, 94
PA3084 PA3084 hypothetical protein (NCBI) 94, 195
PA3085 PA3085 hypothetical protein (NCBI) 94, 477
PA3088 ppnK inorganic polyphosphate/ATP-NAD kinase (NCBI) 94, 477
PA3634 PA3634 hypothetical protein (NCBI) 14, 396
PA3805 pilF type 4 fimbrial biogenesis protein PilF (NCBI) 94, 329
PA3808 PA3808 hypothetical protein (NCBI) 14, 409
PA3853 PA3853 probable transferase (NCBI) 14, 253
PA4424 PA4424 hypothetical protein (NCBI) 94, 263
PA4452 PA4452 hypothetical protein (NCBI) 14, 341
PA4478 PA4478 Maf-like protein (NCBI) 14, 28
PA4482 gatC aspartyl/glutamyl-tRNA amidotransferase subunit C (NCBI) 14, 271
PA4485 PA4485 hypothetical protein (NCBI) 14, 353
PA4617 PA4617 hypothetical protein (NCBI) 14, 60
PA4695 ilvH acetolactate synthase III small subunit (NCBI) 94, 543
PA5109 PA5109 hypothetical protein (NCBI) 14, 353
PA5134 PA5134 probable carboxyl-terminal protease (NCBI) 94, 499
PA5236 PA5236 hypothetical protein (NCBI) 14, 353
Gene Page Help

Network Tab

If the gene is associated with a module(s), its connection to given modules along with other members of that module are shown as network by using CytoscapeWeb. In this view, each green colored circular nodes represent module member genes, purple colored diamonds represent module motifs and red triangles represent regulators. Each node is connected to module (Bicluster) via edges. This representation provides quick overview of all genes, regulators and motifs for modules. It also allows one to see shared genes/motifs/regulators among diferent modules.

Network representation is interactive. You can zoom in/out and move nodes/edges around. Clicking on a node will open up a window to give more details. For genes, Locus tag, organism, genomic coordinates, NCBI gene ID, whether it is transcription factor or not and any associated functional information will be shown. For regulators, number of modules are shown in addition to gene details. For motifs, e-value, consensus sequence and sequence logo will be shown. For modules, expression profile plot, motif information, functional associations and motif locations for each member of the module will be shown.
You can pin information boxes by using button in the box title and open up additional ones on the same screen for comparative analysis.

Regulation Tab

Regulation tab for each gene includes regulatory influences such as environmental factors or transcription factors or their combinations identified by regulatory network inference algorithms.

If the gene is a member of a module, regulators influencing that module are also considered to regulate the gene. Regulators table list total number of regulatory influences, regulators, modules and type of the influence.

You can see description of the regulator inside the tooltip when you mouseover. In certain cases the regulatory influence is predicted to be the result of the combination of two influences. These are indicated as combiner in the column labeled "Operator".

For transcription factors, an additional table next to regulator table will be show. This table show modules that are influenced by the transcription factor.

Motifs Tab

Network inference algorithm uses de novo motif prediction for assigning genes to modules. If there are any motifs identified in the upstream region of a gene, the motif will be shown here. For each motif sequence logo, consensus and e-value will be shown.

Functions Tab

Identification of functional enrichment for the module members is important in associating predicted motifs and regulatory influences with pathways. As described above, the network inference pipeline includes a functional enrichment module by which hypergeometric p-values are used to identify over representation of functional ontology terms among module members.

Network Portal presents functional ontologies from KEGG, GO, TIGRFAM, and COG as separate tables that include function name, type, corrected and uncorrected hypergeometric p-values, and the number of genes assigned to this category out of total number of genes in the module.

Module Members Tab

Identity of gene members in a module may help to identify potential interactions between different functional modules. Therefore, neighbor genes that share the same module(s) with gene under consideration are shown here. For each memebr, gene name, description and modules that contain it are listed.

Help Tab

This help page. More general help can be accessed by clicking help menu in the main navigation bar.

Social Tab

Network Portal is designed to promote collaboration through social interactions. Therefore interested researchers can share information, questions and updates for a particular gene.

Users can use their Disqus, Facebook, Twitter or Google accounts to connect to this page (We recommend Google). Each module and gene page includes comments tab that lists history of the interactions for that gene. You can browse the history, make updates, raise questions and share these activities with social web.

In the next releases of the network portal, we are planning to create personal space for each user where you can share you space that contains all the analysis steps you did along with relevant information.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend
Comments for PA1843
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Gene Help

Overview

Gene landing pages present genomic, functional, and regulatory information for individual genes. A circular visualization displays connections between the selected gene and genes in the same modules, with as edges drawn between the respective coordinates of the whole genome.

The gene page also lists functional ontology assignments, module membership, and motifs associated with these modules. Genes in the network inherit regulatory influences from the modules to which they belong. Therefore, the regulatory information for each gene is a collection of all regulatory influences on these modules. These are listed as a table that includes influence name, type, and target module. If the gene is a transcription factor, its target modules are also displayed in a table that provides residual values and number of genes.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend