Organism : Pseudomonas aeruginosa | Module List :
PA2596

hypothetical protein (NCBI)

CircVis
Functional Annotations (8)
Function System
ABC-type nitrate/sulfonate/bicarbonate transport systems, periplasmic components cog/ cog
transporter activity go/ molecular_function
sulfur compound metabolic process go/ biological_process
transport go/ biological_process
membrane go/ cellular_component
outer membrane-bounded periplasmic space go/ cellular_component
ABC transporters kegg/ kegg pathway
SsuA_fam tigr/ tigrfam
GeneModule member RegulatorRegulator MotifMotif

Cytoscape Web
Regulation information for PA2596
(Mouseover regulator name to see its description)

PA2596 is regulated by 22 influences and regulates 0 modules.
Regulators for PA2596 (22)
Regulator Module Operator
PA0701 241 tf
PA1153 241 tf
PA1223 241 tf
PA1261 241 tf
PA2005 241 tf
PA2050 241 tf
PA3381 241 tf
PA3433 241 tf
PA4341 241 tf
PA4914 241 tf
PA5032 241 tf
PA5431 241 tf
PA0564 497 tf
PA0701 497 tf
PA2050 497 tf
PA2704 497 tf
PA3133 497 tf
PA3391 497 tf
PA3420 497 tf
PA5032 497 tf
PA5293 497 tf
PA5431 497 tf

Warning: PA2596 Does not regulate any modules!

Motif information (de novo identified motifs for modules)

There are 4 motifs predicted.

Motif Table (4)
Motif Id e-value Consensus Motif Logo
3310 3.80e+04 CGgaTaccCCAGatactC.CG
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3311 2.80e+04 AcGccT.TCctCacTG
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3808 1.60e-05 CCGG.AAaTgGtC.gCtaGattcT
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3809 6.40e-04 Attg..GgggAATtc
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Motif Help

Transcription factor binding motifs help to elucidate regulatory mechanism. cMonkey integrates powerful de novo motif detection to identify conditionally co-regulated sets of genes. De novo predicted motifs for each module are listed in the module page as motif logo images along with associated prediction statistics (e-values). The main module page also shows the location of these motifs within the upstream sequences of the module member genes.

Motifs of interest can be broadcasted to RegPredict (currently only available for Desulfovibrio vulgaris Hildenborough) in order to compare conservation in similar species. This integrated motif prediction and comparative analysis provides an additional checkpoint for regulatory motif prediction confidence.

Motif e-value: cMonkey tries to identify two motifs per modules in the upstream sequences of the module member genes. Motif e-value is an indicative of the motif co-occurences between the members of the module.Smaller e-values are indicative of significant sequence motifs. Our experience showed that e-values smaller than 10 are generally indicative of significant motifs.

Functional Enrichment for PA2596

PA2596 is enriched for 8 functions in 3 categories.
Enrichment Table (8)
Function System
ABC-type nitrate/sulfonate/bicarbonate transport systems, periplasmic components cog/ cog
transporter activity go/ molecular_function
sulfur compound metabolic process go/ biological_process
transport go/ biological_process
membrane go/ cellular_component
outer membrane-bounded periplasmic space go/ cellular_component
ABC transporters kegg/ kegg pathway
SsuA_fam tigr/ tigrfam
Module neighborhood information for PA2596

PA2596 has total of 45 gene neighbors in modules 241, 497
Gene neighbors (45)
Gene Common Name Description Module membership
PA0136 PA0136 probable ATP-binding component of ABC transporter (NCBI) 481, 497
PA0138 PA0138 probable permease of ABC transporter (NCBI) 481, 497
PA0186 PA0186 probable binding protein component of ABC transporter (NCBI) 362, 497
PA0602 PA0602 probable binding protein component of ABC transporter (NCBI) 31, 241
PA0714 PA0714 hypothetical protein (NCBI) 241, 311
PA0847 PA0847 hypothetical protein (NCBI) 241, 523
PA1046 PA1046 hypothetical protein (NCBI) 241, 375
PA1133 PA1133 hypothetical protein (NCBI) 171, 241
PA1153 PA1153 hypothetical protein (NCBI) 104, 241
PA1234 PA1234 hypothetical protein (NCBI) 20, 241
PA1567 PA1567 hypothetical protein (NCBI) 16, 497
PA1636 kdpD two-component sensor KdpD (NCBI) 241, 313
PA1637 kdpE two-component response regulator KdpE (NCBI) 241, 313
PA1671 stk1 serine-threonine kinase Stk1 (NCBI) 33, 497
PA2005 PA2005 probable transcriptional regulator (NCBI) 241, 489
PA2596 PA2596 hypothetical protein (NCBI) 241, 497
PA2704 PA2704 probable transcriptional regulator (NCBI) 445, 497
PA2916 PA2916 hypothetical protein (NCBI) 333, 497
PA3125 PA3125 hypothetical protein (NCBI) 196, 497
PA3358 PA3358 hypothetical protein (NCBI) 171, 241
PA3393 nosD NosD protein (NCBI) 458, 497
PA3394 nosF NosF protein (NCBI) 458, 497
PA3395 nosY NosY protein (NCBI) 36, 497
PA3396 nosL NosL protein (NCBI) 74, 497
PA3669 PA3669 hypothetical protein (NCBI) 104, 241
PA3670 PA3670 hypothetical protein (NCBI) 104, 241
PA3783 PA3783 hypothetical protein (NCBI) 175, 241
PA3886 PA3886 hypothetical protein (NCBI) 187, 241
PA4093 PA4093 hypothetical protein (NCBI) 241, 489
PA4347 PA4347 hypothetical protein (NCBI) 241, 253
PA4541 PA4541 hypothetical protein (NCBI) 97, 497
PA4586 PA4586 hypothetical protein (NCBI) 192, 497
PA4827 PA4827 arylamine N-acetyltransferase (NCBI) 292, 497
PA4828 PA4828 hypothetical protein (NCBI) 292, 497
PA4921 PA4921 hypothetical protein (NCBI) 206, 241
PA4982 PA4982 probable two-component sensor (NCBI) 497, 507
PA5089 PA5089 hypothetical protein (NCBI) 241, 473
PA5283 PA5283 probable transcriptional regulator (NCBI) 241, 434
PA5430 PA5430 hypothetical protein (NCBI) 268, 497
PA5431 PA5431 probable transcriptional regulator (NCBI) 497, 507
PA5432 PA5432 probable acetyltransferase (NCBI) 469, 497
PA5433 PA5433 hypothetical protein (NCBI) 246, 497
PA5444 PA5444 hypothetical protein (NCBI) 206, 241
PA5480 PA5480 hypothetical protein (NCBI) 71, 497
PA5514 poxB probable beta-lactamase (NCBI) 241, 469
Gene Page Help

Network Tab

If the gene is associated with a module(s), its connection to given modules along with other members of that module are shown as network by using CytoscapeWeb. In this view, each green colored circular nodes represent module member genes, purple colored diamonds represent module motifs and red triangles represent regulators. Each node is connected to module (Bicluster) via edges. This representation provides quick overview of all genes, regulators and motifs for modules. It also allows one to see shared genes/motifs/regulators among diferent modules.

Network representation is interactive. You can zoom in/out and move nodes/edges around. Clicking on a node will open up a window to give more details. For genes, Locus tag, organism, genomic coordinates, NCBI gene ID, whether it is transcription factor or not and any associated functional information will be shown. For regulators, number of modules are shown in addition to gene details. For motifs, e-value, consensus sequence and sequence logo will be shown. For modules, expression profile plot, motif information, functional associations and motif locations for each member of the module will be shown.
You can pin information boxes by using button in the box title and open up additional ones on the same screen for comparative analysis.

Regulation Tab

Regulation tab for each gene includes regulatory influences such as environmental factors or transcription factors or their combinations identified by regulatory network inference algorithms.

If the gene is a member of a module, regulators influencing that module are also considered to regulate the gene. Regulators table list total number of regulatory influences, regulators, modules and type of the influence.

You can see description of the regulator inside the tooltip when you mouseover. In certain cases the regulatory influence is predicted to be the result of the combination of two influences. These are indicated as combiner in the column labeled "Operator".

For transcription factors, an additional table next to regulator table will be show. This table show modules that are influenced by the transcription factor.

Motifs Tab

Network inference algorithm uses de novo motif prediction for assigning genes to modules. If there are any motifs identified in the upstream region of a gene, the motif will be shown here. For each motif sequence logo, consensus and e-value will be shown.

Functions Tab

Identification of functional enrichment for the module members is important in associating predicted motifs and regulatory influences with pathways. As described above, the network inference pipeline includes a functional enrichment module by which hypergeometric p-values are used to identify over representation of functional ontology terms among module members.

Network Portal presents functional ontologies from KEGG, GO, TIGRFAM, and COG as separate tables that include function name, type, corrected and uncorrected hypergeometric p-values, and the number of genes assigned to this category out of total number of genes in the module.

Module Members Tab

Identity of gene members in a module may help to identify potential interactions between different functional modules. Therefore, neighbor genes that share the same module(s) with gene under consideration are shown here. For each memebr, gene name, description and modules that contain it are listed.

Help Tab

This help page. More general help can be accessed by clicking help menu in the main navigation bar.

Social Tab

Network Portal is designed to promote collaboration through social interactions. Therefore interested researchers can share information, questions and updates for a particular gene.

Users can use their Disqus, Facebook, Twitter or Google accounts to connect to this page (We recommend Google). Each module and gene page includes comments tab that lists history of the interactions for that gene. You can browse the history, make updates, raise questions and share these activities with social web.

In the next releases of the network portal, we are planning to create personal space for each user where you can share you space that contains all the analysis steps you did along with relevant information.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend
Comments for PA2596
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Gene Help

Overview

Gene landing pages present genomic, functional, and regulatory information for individual genes. A circular visualization displays connections between the selected gene and genes in the same modules, with as edges drawn between the respective coordinates of the whole genome.

The gene page also lists functional ontology assignments, module membership, and motifs associated with these modules. Genes in the network inherit regulatory influences from the modules to which they belong. Therefore, the regulatory information for each gene is a collection of all regulatory influences on these modules. These are listed as a table that includes influence name, type, and target module. If the gene is a transcription factor, its target modules are also displayed in a table that provides residual values and number of genes.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend