Organism : Pseudomonas aeruginosa | Module List :
PA3287

hypothetical protein (NCBI)

CircVis
Functional Annotations (1)
Function System
FOG: Ankyrin repeat cog/ cog
GeneModule member RegulatorRegulator MotifMotif

Cytoscape Web
Regulation information for PA3287
(Mouseover regulator name to see its description)

PA3287 is regulated by 43 influences and regulates 0 modules.
Regulators for PA3287 (43)
Regulator Module Operator
PA0159 152 tf
PA0181 152 tf
PA0236 152 tf
PA0436 152 tf
PA0477 152 tf
PA0512 152 tf
PA0535 152 tf
PA0893 152 tf
PA1328 152 tf
PA1455 152 tf
PA1539 152 tf
PA1633 152 tf
PA1826 152 tf
PA2005 152 tf
PA2118 152 tf
PA2273 152 tf
PA2299 152 tf
PA2511 152 tf
PA2586 152 tf
PA2802 152 tf
PA3815 152 tf
PA3932 152 tf
PA4354 152 tf
PA5059 152 tf
PA5105 152 tf
PA5157 152 tf
PA5166 152 tf
PA5274 152 tf
PA5288 152 tf
PA5356 152 tf
PA0533 432 tf
PA0791 432 tf
PA1136 432 tf
PA1264 432 tf
PA1399 432 tf
PA1539 432 tf
PA2206 432 tf
PA3973 432 tf
PA4269 432 tf
PA4703 432 tf
PA4914 432 tf
PA5059 432 tf
PA5105 432 tf

Warning: PA3287 Does not regulate any modules!

Motif information (de novo identified motifs for modules)

There are 4 motifs predicted.

Motif Table (4)
Motif Id e-value Consensus Motif Logo
3134 4.20e-07 a..T.Aaac.tcaagaaAaa.taa
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3135 6.40e+01 aTAtTCat
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3684 2.00e-03 TtCccatcGAAaAcCgccgcaga
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3685 2.70e+02 TGCCAGGCTgGCCaGacCTACCC
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Motif Help

Transcription factor binding motifs help to elucidate regulatory mechanism. cMonkey integrates powerful de novo motif detection to identify conditionally co-regulated sets of genes. De novo predicted motifs for each module are listed in the module page as motif logo images along with associated prediction statistics (e-values). The main module page also shows the location of these motifs within the upstream sequences of the module member genes.

Motifs of interest can be broadcasted to RegPredict (currently only available for Desulfovibrio vulgaris Hildenborough) in order to compare conservation in similar species. This integrated motif prediction and comparative analysis provides an additional checkpoint for regulatory motif prediction confidence.

Motif e-value: cMonkey tries to identify two motifs per modules in the upstream sequences of the module member genes. Motif e-value is an indicative of the motif co-occurences between the members of the module.Smaller e-values are indicative of significant sequence motifs. Our experience showed that e-values smaller than 10 are generally indicative of significant motifs.

Functional Enrichment for PA3287

PA3287 is enriched for 1 functions in 3 categories.
Enrichment Table (1)
Function System
FOG: Ankyrin repeat cog/ cog
Module neighborhood information for PA3287

PA3287 has total of 52 gene neighbors in modules 152, 432
Gene neighbors (52)
Gene Common Name Description Module membership
PA0051 phzH potential phenazine-modifying enzyme (NCBI) 152, 292
PA0140 ahpF alkyl hydroperoxide reductase subunit F (NCBI) 152, 224
PA0187 PA0187 hypothetical protein (NCBI) 326, 432
PA0229 pcaT dicarboxylic acid transporter PcaT (NCBI) 104, 432
PA0534 PA0534 hypothetical protein (NCBI) 152, 212
PA0535 PA0535 probable transcriptional regulator (NCBI) 152, 212
PA0741 PA0741 hypothetical protein (NCBI) 432, 475
PA0791 PA0791 probable transcriptional regulator (NCBI) 136, 432
PA0848 PA0848 probable alkyl hydroperoxide reductase (NCBI) 152, 550
PA0849 trxB2 thioredoxin reductase 2 (NCBI) 152, 187
PA1136 PA1136 probable transcriptional regulator (NCBI) 323, 432
PA1208 PA1208 hypothetical protein (NCBI) 3, 432
PA1283 PA1283 probable transcriptional regulator (NCBI) 79, 432
PA1362 PA1362 hypothetical protein (NCBI) 373, 432
PA1433 PA1433 hypothetical protein (NCBI) 432, 538
PA1434 PA1434 hypothetical protein (NCBI) 432, 538
PA1531 PA1531 hypothetical protein (NCBI) 196, 432
PA1604 PA1604 hypothetical protein (NCBI) 34, 432
PA1747 PA1747 hypothetical protein (NCBI) 373, 432
PA1927 metE 5-methyltetrahydropteroyltriglutamate-- homocysteine methyltransferase (NCBI) 152, 236
PA1968 PA1968 hypothetical protein (NCBI) 208, 432
PA2274 PA2274 hypothetical protein (NCBI) 152, 309
PA2698 PA2698 probable hydrolase (NCBI) 152, 268
PA2794 PA2794 hypothetical protein (NCBI) 152, 188
PA2795 PA2795 hypothetical protein (NCBI) 152, 326
PA2868 PA2868 hypothetical protein (NCBI) 39, 432
PA2949 PA2949 probable lipase (NCBI) 313, 432
PA3032 snr1 cytochrome c Snr1 (NCBI) 74, 432
PA3237 PA3237 hypothetical protein (NCBI) 152, 538
PA3287 PA3287 hypothetical protein (NCBI) 152, 432
PA3499 PA3499 hypothetical protein (NCBI) 373, 432
PA3600 PA3600 hypothetical protein (NCBI) 152, 361
PA3601 PA3601 hypothetical protein (NCBI) 152, 361
PA3681 PA3681 hypothetical protein (NCBI) 394, 432
PA3787 PA3787 hypothetical protein (NCBI) 432, 469
PA3973 PA3973 probable transcriptional regulator (NCBI) 163, 432
PA4205 mexG hypothetical protein (NCBI) 152, 309
PA4206 mexH probable Resistance-Nodulation-Cell Division (RND) efflux membrane fusion protein precursor (NCBI) 152, 309
PA4207 mexI probable Resistance-Nodulation-Cell Division (RND) efflux transporter (NCBI) 152, 309
PA4208 opmD probable outer membrane protein precursor (NCBI) 152, 309
PA4210 phzA1 probable phenazine biosynthesis protein (NCBI) 152, 250
PA4612 PA4612 hypothetical protein (NCBI) 152, 344
PA4613 katB catalase (NCBI) 152, 313
PA4623 PA4623 hypothetical protein (NCBI) 108, 152
PA4653 PA4653 hypothetical protein (NCBI) 367, 432
PA4881 PA4881 hypothetical protein (NCBI) 108, 152
PA4975 PA4975 NAD(P)H quinone oxidoreductase (NCBI) 97, 432
PA5180 PA5180 hypothetical protein (NCBI) 152, 425
PA5181 PA5181 probable oxidoreductase (NCBI) 152, 425
PA5394 cls cardiolipin synthetase (NCBI) 373, 432
PA5395 PA5395 hypothetical protein (NCBI) 84, 432
PA5520 PA5520 hypothetical protein (NCBI) 136, 432
Gene Page Help

Network Tab

If the gene is associated with a module(s), its connection to given modules along with other members of that module are shown as network by using CytoscapeWeb. In this view, each green colored circular nodes represent module member genes, purple colored diamonds represent module motifs and red triangles represent regulators. Each node is connected to module (Bicluster) via edges. This representation provides quick overview of all genes, regulators and motifs for modules. It also allows one to see shared genes/motifs/regulators among diferent modules.

Network representation is interactive. You can zoom in/out and move nodes/edges around. Clicking on a node will open up a window to give more details. For genes, Locus tag, organism, genomic coordinates, NCBI gene ID, whether it is transcription factor or not and any associated functional information will be shown. For regulators, number of modules are shown in addition to gene details. For motifs, e-value, consensus sequence and sequence logo will be shown. For modules, expression profile plot, motif information, functional associations and motif locations for each member of the module will be shown.
You can pin information boxes by using button in the box title and open up additional ones on the same screen for comparative analysis.

Regulation Tab

Regulation tab for each gene includes regulatory influences such as environmental factors or transcription factors or their combinations identified by regulatory network inference algorithms.

If the gene is a member of a module, regulators influencing that module are also considered to regulate the gene. Regulators table list total number of regulatory influences, regulators, modules and type of the influence.

You can see description of the regulator inside the tooltip when you mouseover. In certain cases the regulatory influence is predicted to be the result of the combination of two influences. These are indicated as combiner in the column labeled "Operator".

For transcription factors, an additional table next to regulator table will be show. This table show modules that are influenced by the transcription factor.

Motifs Tab

Network inference algorithm uses de novo motif prediction for assigning genes to modules. If there are any motifs identified in the upstream region of a gene, the motif will be shown here. For each motif sequence logo, consensus and e-value will be shown.

Functions Tab

Identification of functional enrichment for the module members is important in associating predicted motifs and regulatory influences with pathways. As described above, the network inference pipeline includes a functional enrichment module by which hypergeometric p-values are used to identify over representation of functional ontology terms among module members.

Network Portal presents functional ontologies from KEGG, GO, TIGRFAM, and COG as separate tables that include function name, type, corrected and uncorrected hypergeometric p-values, and the number of genes assigned to this category out of total number of genes in the module.

Module Members Tab

Identity of gene members in a module may help to identify potential interactions between different functional modules. Therefore, neighbor genes that share the same module(s) with gene under consideration are shown here. For each memebr, gene name, description and modules that contain it are listed.

Help Tab

This help page. More general help can be accessed by clicking help menu in the main navigation bar.

Social Tab

Network Portal is designed to promote collaboration through social interactions. Therefore interested researchers can share information, questions and updates for a particular gene.

Users can use their Disqus, Facebook, Twitter or Google accounts to connect to this page (We recommend Google). Each module and gene page includes comments tab that lists history of the interactions for that gene. You can browse the history, make updates, raise questions and share these activities with social web.

In the next releases of the network portal, we are planning to create personal space for each user where you can share you space that contains all the analysis steps you did along with relevant information.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend
Comments for PA3287
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Gene Help

Overview

Gene landing pages present genomic, functional, and regulatory information for individual genes. A circular visualization displays connections between the selected gene and genes in the same modules, with as edges drawn between the respective coordinates of the whole genome.

The gene page also lists functional ontology assignments, module membership, and motifs associated with these modules. Genes in the network inherit regulatory influences from the modules to which they belong. Therefore, the regulatory information for each gene is a collection of all regulatory influences on these modules. These are listed as a table that includes influence name, type, and target module. If the gene is a transcription factor, its target modules are also displayed in a table that provides residual values and number of genes.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend