Organism : Pseudomonas aeruginosa | Module List :
PA4756 carB

carbamoyl-phosphate synthase large subunit (NCBI)

CircVis
Functional Annotations (10)
Function System
Carbamoylphosphate synthase large subunit (split gene in MJ) cog/ cog
carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity go/ molecular_function
ATP binding go/ molecular_function
cytoplasm go/ cellular_component
arginine biosynthetic process go/ biological_process
pyrimidine base biosynthetic process go/ biological_process
Pyrimidine metabolism kegg/ kegg pathway
Alanine aspartate and glutamate metabolism kegg/ kegg pathway
Metabolic pathways kegg/ kegg pathway
CPSaseII_lrg tigr/ tigrfam
GeneModule member RegulatorRegulator MotifMotif

Cytoscape Web
Regulation information for PA4756
(Mouseover regulator name to see its description)

PA4756 is regulated by 29 influences and regulates 0 modules.
Regulators for PA4756 carB (29)
Regulator Module Operator
PA0179 453 tf
PA0275 453 tf
PA0393 453 tf
PA0652 453 tf
PA0893 453 tf
PA0961 453 tf
PA1945 453 tf
PA2957 453 tf
PA3002 453 tf
PA3225 453 tf
PA4052 453 tf
PA4238 453 tf
PA4269 453 tf
PA4270 453 tf
PA4275 453 tf
PA4745 453 tf
PA4755 453 tf
PA5324 453 tf
PA5562 453 tf
PA0133 150 tf
PA0763 150 tf
PA0890 150 tf
PA0893 150 tf
PA3002 150 tf
PA3804 150 tf
PA4269 150 tf
PA4270 150 tf
PA4547 150 tf
PA5562 150 tf

Warning: PA4756 Does not regulate any modules!

Motif information (de novo identified motifs for modules)

There are 4 motifs predicted.

Motif Table (4)
Motif Id e-value Consensus Motif Logo
3130 1.60e+04 ccGtcG.gGAcggCAacgAtt
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3131 9.70e+03 tTTCCGgC
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3724 1.10e-02 cAtgCtaCGC.GCtTtTcGt
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3725 9.30e+02 tTTTtaA
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Motif Help

Transcription factor binding motifs help to elucidate regulatory mechanism. cMonkey integrates powerful de novo motif detection to identify conditionally co-regulated sets of genes. De novo predicted motifs for each module are listed in the module page as motif logo images along with associated prediction statistics (e-values). The main module page also shows the location of these motifs within the upstream sequences of the module member genes.

Motifs of interest can be broadcasted to RegPredict (currently only available for Desulfovibrio vulgaris Hildenborough) in order to compare conservation in similar species. This integrated motif prediction and comparative analysis provides an additional checkpoint for regulatory motif prediction confidence.

Motif e-value: cMonkey tries to identify two motifs per modules in the upstream sequences of the module member genes. Motif e-value is an indicative of the motif co-occurences between the members of the module.Smaller e-values are indicative of significant sequence motifs. Our experience showed that e-values smaller than 10 are generally indicative of significant motifs.

Functional Enrichment for PA4756

PA4756 is enriched for 10 functions in 3 categories.
Enrichment Table (10)
Function System
Carbamoylphosphate synthase large subunit (split gene in MJ) cog/ cog
carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity go/ molecular_function
ATP binding go/ molecular_function
cytoplasm go/ cellular_component
arginine biosynthetic process go/ biological_process
pyrimidine base biosynthetic process go/ biological_process
Pyrimidine metabolism kegg/ kegg pathway
Alanine aspartate and glutamate metabolism kegg/ kegg pathway
Metabolic pathways kegg/ kegg pathway
CPSaseII_lrg tigr/ tigrfam
Module neighborhood information for PA4756

PA4756 has total of 28 gene neighbors in modules 150, 453
Gene neighbors (28)
Gene Common Name Description Module membership
PA0008 glyS glycyl-tRNA synthetase beta subunit (NCBI) 274, 453
PA0009 glyQ glycyl-tRNA synthetase alpha subunit (NCBI) 106, 453
PA0370 PA0370 hypothetical protein (NCBI) 37, 150
PA0891 PA0891 hypothetical protein (NCBI) 150, 160
PA0892 aotP arginine/ornithine transport protein AotP (NCBI) 150, 160
PA0904 lysC aspartate kinase (NCBI) 69, 453
PA2740 pheS phenylalanyl-tRNA synthetase alpha subunit (NCBI) 260, 453
PA3107 metZ O-succinylhomoserine sulfhydrylase (NCBI) 150, 160
PA3163 cmk cytidylate kinase (NCBI) 37, 150
PA3653 frr ribosome releasing factor (NCBI) 259, 453
PA3654 pyrH uridylate kinase (NCBI) 106, 453
PA3700 lysS lysyl-tRNA synthetase (NCBI) 90, 453
PA3802 hisS histidyl-tRNA synthetase (NCBI) 150, 160
PA3803 ispG 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (NCBI) 150, 160
PA3821 secD protein export protein SecD (NCBI) 277, 453
PA4269 rpoC DNA-directed RNA polymerase beta' subunit (NCBI) 150, 259
PA4270 rpoB DNA-directed RNA polymerase beta subunit (NCBI) 150, 259
PA4402 argJ bifunctional ornithine acetyltransferase/N-acetylglutamate synthase protein (NCBI) 150, 389
PA4483 gatA glutamyl-tRNA amidotransferase subunit A (NCBI) 453, 543
PA4484 gatB aspartyl/glutamyl-tRNA amidotransferase subunit B (NCBI) 150, 244
PA4558 PA4558 probable peptidyl-prolyl cis-trans isomerase, FkbP-type (NCBI) 150, 403
PA4754 PA4754 hypothetical protein (NCBI) 453, 478
PA4756 carB carbamoyl-phosphate synthase large subunit (NCBI) 150, 453
PA4757 PA4757 hypothetical protein (NCBI) 270, 453
PA4758 carA carbamoyl-phosphate synthase small subunit (NCBI) 106, 453
PA5046 PA5046 malic enzyme (NCBI) 329, 453
PA5147 mutY A / G specific adenine glycosylase (NCBI) 150, 160
PA5568 PA5568 putative inner membrane protein translocase component YidC (NCBI) 426, 453
Gene Page Help

Network Tab

If the gene is associated with a module(s), its connection to given modules along with other members of that module are shown as network by using CytoscapeWeb. In this view, each green colored circular nodes represent module member genes, purple colored diamonds represent module motifs and red triangles represent regulators. Each node is connected to module (Bicluster) via edges. This representation provides quick overview of all genes, regulators and motifs for modules. It also allows one to see shared genes/motifs/regulators among diferent modules.

Network representation is interactive. You can zoom in/out and move nodes/edges around. Clicking on a node will open up a window to give more details. For genes, Locus tag, organism, genomic coordinates, NCBI gene ID, whether it is transcription factor or not and any associated functional information will be shown. For regulators, number of modules are shown in addition to gene details. For motifs, e-value, consensus sequence and sequence logo will be shown. For modules, expression profile plot, motif information, functional associations and motif locations for each member of the module will be shown.
You can pin information boxes by using button in the box title and open up additional ones on the same screen for comparative analysis.

Regulation Tab

Regulation tab for each gene includes regulatory influences such as environmental factors or transcription factors or their combinations identified by regulatory network inference algorithms.

If the gene is a member of a module, regulators influencing that module are also considered to regulate the gene. Regulators table list total number of regulatory influences, regulators, modules and type of the influence.

You can see description of the regulator inside the tooltip when you mouseover. In certain cases the regulatory influence is predicted to be the result of the combination of two influences. These are indicated as combiner in the column labeled "Operator".

For transcription factors, an additional table next to regulator table will be show. This table show modules that are influenced by the transcription factor.

Motifs Tab

Network inference algorithm uses de novo motif prediction for assigning genes to modules. If there are any motifs identified in the upstream region of a gene, the motif will be shown here. For each motif sequence logo, consensus and e-value will be shown.

Functions Tab

Identification of functional enrichment for the module members is important in associating predicted motifs and regulatory influences with pathways. As described above, the network inference pipeline includes a functional enrichment module by which hypergeometric p-values are used to identify over representation of functional ontology terms among module members.

Network Portal presents functional ontologies from KEGG, GO, TIGRFAM, and COG as separate tables that include function name, type, corrected and uncorrected hypergeometric p-values, and the number of genes assigned to this category out of total number of genes in the module.

Module Members Tab

Identity of gene members in a module may help to identify potential interactions between different functional modules. Therefore, neighbor genes that share the same module(s) with gene under consideration are shown here. For each memebr, gene name, description and modules that contain it are listed.

Help Tab

This help page. More general help can be accessed by clicking help menu in the main navigation bar.

Social Tab

Network Portal is designed to promote collaboration through social interactions. Therefore interested researchers can share information, questions and updates for a particular gene.

Users can use their Disqus, Facebook, Twitter or Google accounts to connect to this page (We recommend Google). Each module and gene page includes comments tab that lists history of the interactions for that gene. You can browse the history, make updates, raise questions and share these activities with social web.

In the next releases of the network portal, we are planning to create personal space for each user where you can share you space that contains all the analysis steps you did along with relevant information.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend
Comments for PA4756
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Gene Help

Overview

Gene landing pages present genomic, functional, and regulatory information for individual genes. A circular visualization displays connections between the selected gene and genes in the same modules, with as edges drawn between the respective coordinates of the whole genome.

The gene page also lists functional ontology assignments, module membership, and motifs associated with these modules. Genes in the network inherit regulatory influences from the modules to which they belong. Therefore, the regulatory information for each gene is a collection of all regulatory influences on these modules. These are listed as a table that includes influence name, type, and target module. If the gene is a transcription factor, its target modules are also displayed in a table that provides residual values and number of genes.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend