Organism : Pseudomonas aeruginosa | Module List :
PA5464

hypothetical protein (NCBI)

CircVis
Functional Annotations (3)
Function System
proteolysis go/ biological_process
metallopeptidase activity go/ molecular_function
zinc ion binding go/ molecular_function
GeneModule member RegulatorRegulator MotifMotif

Cytoscape Web
Regulation information for PA5464
(Mouseover regulator name to see its description)

PA5464 is regulated by 37 influences and regulates 0 modules.
Regulators for PA5464 (37)
Regulator Module Operator
PA0152 247 tf
PA0764 247 tf
PA0961 247 tf
PA1290 247 tf
PA1363 247 tf
PA1853 247 tf
PA2047 247 tf
PA2056 247 tf
PA2115 247 tf
PA2737 247 tf
PA2921 247 tf
PA2957 247 tf
PA3921 247 tf
PA3995 247 tf
PA4182 247 tf
PA4769 247 tf
PA4853 247 tf
PA4890 247 tf
PA5059 247 tf
PA5253 247 tf
PA5356 247 tf
PA5403 247 tf
PA5431 247 tf
PA5437 247 tf
PA5550 247 tf
PA0179 249 tf
PA1397 249 tf
PA2047 249 tf
PA2957 249 tf
PA3322 249 tf
PA3604 249 tf
PA3622 249 tf
PA4238 249 tf
PA4275 249 tf
PA4451 249 tf
PA4745 249 tf
PA4853 249 tf

Warning: PA5464 Does not regulate any modules!

Motif information (de novo identified motifs for modules)

There are 4 motifs predicted.

Motif Table (4)
Motif Id e-value Consensus Motif Logo
3322 5.10e+03 AgaaTGCC
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3323 5.80e+03 gTTC.A
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3326 2.00e+03 TATAATG
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3327 7.60e+02 aAcCAtT
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Motif Help

Transcription factor binding motifs help to elucidate regulatory mechanism. cMonkey integrates powerful de novo motif detection to identify conditionally co-regulated sets of genes. De novo predicted motifs for each module are listed in the module page as motif logo images along with associated prediction statistics (e-values). The main module page also shows the location of these motifs within the upstream sequences of the module member genes.

Motifs of interest can be broadcasted to RegPredict (currently only available for Desulfovibrio vulgaris Hildenborough) in order to compare conservation in similar species. This integrated motif prediction and comparative analysis provides an additional checkpoint for regulatory motif prediction confidence.

Motif e-value: cMonkey tries to identify two motifs per modules in the upstream sequences of the module member genes. Motif e-value is an indicative of the motif co-occurences between the members of the module.Smaller e-values are indicative of significant sequence motifs. Our experience showed that e-values smaller than 10 are generally indicative of significant motifs.

Functional Enrichment for PA5464

PA5464 is enriched for 3 functions in 2 categories.
Enrichment Table (3)
Function System
proteolysis go/ biological_process
metallopeptidase activity go/ molecular_function
zinc ion binding go/ molecular_function
Module neighborhood information for PA5464

PA5464 has total of 38 gene neighbors in modules 247, 249
Gene neighbors (38)
Gene Common Name Description Module membership
PA0150 PA0150 probable transmembrane sensor (NCBI) 99, 247
PA0252 PA0252 hypothetical protein (NCBI) 218, 247
PA0317 PA0317 hypothetical protein (NCBI) 249, 451
PA0340 PA0340 hypothetical protein (NCBI) 65, 247
PA0419 PA0419 hypothetical protein (NCBI) 229, 247
PA1006 PA1006 hypothetical protein (NCBI) 247, 335
PA1007 PA1007 hypothetical protein (NCBI) 247, 335
PA1360 PA1360 hypothetical protein (NCBI) 247, 344
PA1475 ccmA heme exporter protein CcmA (NCBI) 249, 544
PA1476 ccmB heme exporter protein CcmB (NCBI) 249, 544
PA1477 ccmC heme exporter protein CcmC (NCBI) 249, 544
PA1593 PA1593 hypothetical protein (NCBI) 247, 299
PA1638 PA1638 glutaminase (NCBI) 247, 377
PA1757 thrH homoserine kinase (NCBI) 249, 286
PA1824 PA1824 hypothetical protein (NCBI) 65, 247
PA1846 cti cis/trans isomerase (NCBI) 249, 306
PA2038 PA2038 hypothetical protein (NCBI) 40, 247
PA2039 PA2039 hypothetical protein (NCBI) 247, 299
PA2750 PA2750 hypothetical protein (NCBI) 247, 299
PA2757 PA2757 hypothetical protein (NCBI) 240, 247
PA2789 PA2789 hypothetical protein (NCBI) 247, 434
PA2876 pyrF orotidine 5'-phosphate decarboxylase (NCBI) 247, 422
PA3116 PA3116 aspartate-semialdehyde dehydrogenase (NCBI) 247, 332
PA3411 PA3411 hypothetical protein (NCBI) 247, 377
PA3605 PA3605 hypothetical protein (NCBI) 175, 247
PA3726 PA3726 hypothetical protein (NCBI) 181, 247
PA3766 PA3766 probable aromatic amino acid transporter (NCBI) 58, 247
PA3958 PA3958 hypothetical protein (NCBI) 229, 249
PA4029 PA4029 hypothetical protein (NCBI) 170, 249
PA4030 PA4030 hypothetical protein (NCBI) 170, 249
PA4404 PA4404 hypothetical protein (NCBI) 249, 511
PA4636 PA4636 hypothetical protein (NCBI) 247, 332
PA4700 mrcB penicillin-binding protein 1B (NCBI) 103, 249
PA4845 dipZ thiol:disulfide interchange protein precursor (NCBI) 249, 517
PA5194 PA5194 hypothetical protein (NCBI) 247, 299
PA5464 PA5464 hypothetical protein (NCBI) 247, 249
PA5472 PA5472 hypothetical protein (NCBI) 247, 255
PA5486 PA5486 hypothetical protein (NCBI) 126, 247
Gene Page Help

Network Tab

If the gene is associated with a module(s), its connection to given modules along with other members of that module are shown as network by using CytoscapeWeb. In this view, each green colored circular nodes represent module member genes, purple colored diamonds represent module motifs and red triangles represent regulators. Each node is connected to module (Bicluster) via edges. This representation provides quick overview of all genes, regulators and motifs for modules. It also allows one to see shared genes/motifs/regulators among diferent modules.

Network representation is interactive. You can zoom in/out and move nodes/edges around. Clicking on a node will open up a window to give more details. For genes, Locus tag, organism, genomic coordinates, NCBI gene ID, whether it is transcription factor or not and any associated functional information will be shown. For regulators, number of modules are shown in addition to gene details. For motifs, e-value, consensus sequence and sequence logo will be shown. For modules, expression profile plot, motif information, functional associations and motif locations for each member of the module will be shown.
You can pin information boxes by using button in the box title and open up additional ones on the same screen for comparative analysis.

Regulation Tab

Regulation tab for each gene includes regulatory influences such as environmental factors or transcription factors or their combinations identified by regulatory network inference algorithms.

If the gene is a member of a module, regulators influencing that module are also considered to regulate the gene. Regulators table list total number of regulatory influences, regulators, modules and type of the influence.

You can see description of the regulator inside the tooltip when you mouseover. In certain cases the regulatory influence is predicted to be the result of the combination of two influences. These are indicated as combiner in the column labeled "Operator".

For transcription factors, an additional table next to regulator table will be show. This table show modules that are influenced by the transcription factor.

Motifs Tab

Network inference algorithm uses de novo motif prediction for assigning genes to modules. If there are any motifs identified in the upstream region of a gene, the motif will be shown here. For each motif sequence logo, consensus and e-value will be shown.

Functions Tab

Identification of functional enrichment for the module members is important in associating predicted motifs and regulatory influences with pathways. As described above, the network inference pipeline includes a functional enrichment module by which hypergeometric p-values are used to identify over representation of functional ontology terms among module members.

Network Portal presents functional ontologies from KEGG, GO, TIGRFAM, and COG as separate tables that include function name, type, corrected and uncorrected hypergeometric p-values, and the number of genes assigned to this category out of total number of genes in the module.

Module Members Tab

Identity of gene members in a module may help to identify potential interactions between different functional modules. Therefore, neighbor genes that share the same module(s) with gene under consideration are shown here. For each memebr, gene name, description and modules that contain it are listed.

Help Tab

This help page. More general help can be accessed by clicking help menu in the main navigation bar.

Social Tab

Network Portal is designed to promote collaboration through social interactions. Therefore interested researchers can share information, questions and updates for a particular gene.

Users can use their Disqus, Facebook, Twitter or Google accounts to connect to this page (We recommend Google). Each module and gene page includes comments tab that lists history of the interactions for that gene. You can browse the history, make updates, raise questions and share these activities with social web.

In the next releases of the network portal, we are planning to create personal space for each user where you can share you space that contains all the analysis steps you did along with relevant information.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend
Comments for PA5464
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Gene Help

Overview

Gene landing pages present genomic, functional, and regulatory information for individual genes. A circular visualization displays connections between the selected gene and genes in the same modules, with as edges drawn between the respective coordinates of the whole genome.

The gene page also lists functional ontology assignments, module membership, and motifs associated with these modules. Genes in the network inherit regulatory influences from the modules to which they belong. Therefore, the regulatory information for each gene is a collection of all regulatory influences on these modules. These are listed as a table that includes influence name, type, and target module. If the gene is a transcription factor, its target modules are also displayed in a table that provides residual values and number of genes.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend