Organism : Rhodobacter sphaeroides 2.4.1 | Module List :
RSP_0536 nifX

putative nitrogenase molybdenum-iron protein NifX (NCBI)

CircVis
Functional Annotations (5)
Function System
nitrogen fixation go/ biological_process
cofactor biosynthetic process go/ biological_process
metal cluster binding go/ molecular_function
Nitrogen metabolism kegg/ kegg pathway
nifX tigr/ tigrfam
GeneModule member RegulatorRegulator MotifMotif

Cytoscape Web
Regulation information for RSP_0536
(Mouseover regulator name to see its description)

RSP_0536 is regulated by 32 influences and regulates 0 modules.
Regulators for RSP_0536 nifX (32)
Regulator Module Operator
RSP_0087 332 tf
RSP_0415 332 tf
RSP_0527 332 tf
RSP_0722 332 tf
RSP_1139 332 tf
RSP_1225 332 tf
RSP_1952 332 tf
RSP_2130 332 tf
RSP_2200 332 tf
RSP_2572 332 tf
RSP_2838 332 tf
RSP_2867 332 tf
RSP_2888 332 tf
RSP_3042 332 tf
RSP_3317 332 tf
RSP_3621 332 tf
RSP_0087 226 tf
RSP_0415 226 tf
RSP_0527 226 tf
RSP_0722 226 tf
RSP_1139 226 tf
RSP_1225 226 tf
RSP_1952 226 tf
RSP_2130 226 tf
RSP_2200 226 tf
RSP_2801 226 tf
RSP_2838 226 tf
RSP_2867 226 tf
RSP_2888 226 tf
RSP_3226 226 tf
RSP_3317 226 tf
RSP_3339 226 tf

Warning: RSP_0536 Does not regulate any modules!

Motif information (de novo identified motifs for modules)

There are 4 motifs predicted.

Motif Table (4)
Motif Id e-value Consensus Motif Logo
8170 4.80e-04 CGccaCtTGGCACGcccccTGCTT
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8171 2.70e+02 AAtgcgAaAa
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8370 1.10e-08 caCtTGGCAcGccgccTGCTTct
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8371 8.70e+02 CttTgTcGGAtT
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Motif Help

Transcription factor binding motifs help to elucidate regulatory mechanism. cMonkey integrates powerful de novo motif detection to identify conditionally co-regulated sets of genes. De novo predicted motifs for each module are listed in the module page as motif logo images along with associated prediction statistics (e-values). The main module page also shows the location of these motifs within the upstream sequences of the module member genes.

Motifs of interest can be broadcasted to RegPredict (currently only available for Desulfovibrio vulgaris Hildenborough) in order to compare conservation in similar species. This integrated motif prediction and comparative analysis provides an additional checkpoint for regulatory motif prediction confidence.

Motif e-value: cMonkey tries to identify two motifs per modules in the upstream sequences of the module member genes. Motif e-value is an indicative of the motif co-occurences between the members of the module.Smaller e-values are indicative of significant sequence motifs. Our experience showed that e-values smaller than 10 are generally indicative of significant motifs.

Functional Enrichment for RSP_0536

RSP_0536 is enriched for 5 functions in 4 categories.
Enrichment Table (5)
Function System
nitrogen fixation go/ biological_process
cofactor biosynthetic process go/ biological_process
metal cluster binding go/ molecular_function
Nitrogen metabolism kegg/ kegg pathway
nifX tigr/ tigrfam
Module neighborhood information for RSP_0536

RSP_0536 has total of 21 gene neighbors in modules 226, 332
Gene neighbors (21)
Gene Common Name Description Module membership
RSP_0528 nifW nitrogen fixation protein NifW (NCBI) 226, 332
RSP_0529 nifV Homocitrate synthase (NCBI) 226, 332
RSP_0530 nifS Cysteine desulfurase (NCBI) 226, 332
RSP_0531 nifU Nitrogen fixation protein (NCBI) 226, 332
RSP_0532 RSP_0532 hypothetical protein (NCBI) 226, 332
RSP_0535 RSP_0535 hypothetical protein (NCBI) 226, 332
RSP_0536 nifX putative nitrogenase molybdenum-iron protein NifX (NCBI) 226, 332
RSP_0537 nifN nitrogenase molybdenum-iron protein beta chain, nifN (NCBI) 226, 332
RSP_0538 nifE nifE, nitrogenase molybdenum-cofactor synthesis protein (NCBI) 226, 332
RSP_0539 nifK nitrogenase molybdenum-iron protein beta chain (NCBI) 226, 332
RSP_0540 nifD Nitrogenase iron-molybdenum protein, alpha chain (NCBI) 226, 332
RSP_0541 nifH Nitrogenase iron protein (Nitrogenase component II) (NCBI) 226, 332
RSP_0546 nifB NifB family--FeMo cofactor biosynthesis protein (NCBI) 226, 332
RSP_3189 RSP_3189 4Fe-4S ferredoxin (NCBI) 313, 332
RSP_3192 rnfA Predicted NADH-ubiquinone oxidoreductase; subunit RnfA (NCBI) 226, 332
RSP_3193 rnfB Electron transport complex protein rnfB (NCBI) 226, 332
RSP_3194 rnfC 4Fe-4S ferredoxin, RnfC (NCBI) 226, 332
RSP_3195 rnfD RnfD protein (NCBI) 226, 332
RSP_3196 rnfG Electron transport complex, RnfABCDGE type, G subunit (NCBI) 226, 332
RSP_3197 rnfE rnfE protein precursor (NCBI) 226, 332
RSP_3198 rnfH probable rnfH protein (NCBI) 226, 332
Gene Page Help

Network Tab

If the gene is associated with a module(s), its connection to given modules along with other members of that module are shown as network by using CytoscapeWeb. In this view, each green colored circular nodes represent module member genes, purple colored diamonds represent module motifs and red triangles represent regulators. Each node is connected to module (Bicluster) via edges. This representation provides quick overview of all genes, regulators and motifs for modules. It also allows one to see shared genes/motifs/regulators among diferent modules.

Network representation is interactive. You can zoom in/out and move nodes/edges around. Clicking on a node will open up a window to give more details. For genes, Locus tag, organism, genomic coordinates, NCBI gene ID, whether it is transcription factor or not and any associated functional information will be shown. For regulators, number of modules are shown in addition to gene details. For motifs, e-value, consensus sequence and sequence logo will be shown. For modules, expression profile plot, motif information, functional associations and motif locations for each member of the module will be shown.
You can pin information boxes by using button in the box title and open up additional ones on the same screen for comparative analysis.

Regulation Tab

Regulation tab for each gene includes regulatory influences such as environmental factors or transcription factors or their combinations identified by regulatory network inference algorithms.

If the gene is a member of a module, regulators influencing that module are also considered to regulate the gene. Regulators table list total number of regulatory influences, regulators, modules and type of the influence.

You can see description of the regulator inside the tooltip when you mouseover. In certain cases the regulatory influence is predicted to be the result of the combination of two influences. These are indicated as combiner in the column labeled "Operator".

For transcription factors, an additional table next to regulator table will be show. This table show modules that are influenced by the transcription factor.

Motifs Tab

Network inference algorithm uses de novo motif prediction for assigning genes to modules. If there are any motifs identified in the upstream region of a gene, the motif will be shown here. For each motif sequence logo, consensus and e-value will be shown.

Functions Tab

Identification of functional enrichment for the module members is important in associating predicted motifs and regulatory influences with pathways. As described above, the network inference pipeline includes a functional enrichment module by which hypergeometric p-values are used to identify over representation of functional ontology terms among module members.

Network Portal presents functional ontologies from KEGG, GO, TIGRFAM, and COG as separate tables that include function name, type, corrected and uncorrected hypergeometric p-values, and the number of genes assigned to this category out of total number of genes in the module.

Module Members Tab

Identity of gene members in a module may help to identify potential interactions between different functional modules. Therefore, neighbor genes that share the same module(s) with gene under consideration are shown here. For each memebr, gene name, description and modules that contain it are listed.

Help Tab

This help page. More general help can be accessed by clicking help menu in the main navigation bar.

Social Tab

Network Portal is designed to promote collaboration through social interactions. Therefore interested researchers can share information, questions and updates for a particular gene.

Users can use their Disqus, Facebook, Twitter or Google accounts to connect to this page (We recommend Google). Each module and gene page includes comments tab that lists history of the interactions for that gene. You can browse the history, make updates, raise questions and share these activities with social web.

In the next releases of the network portal, we are planning to create personal space for each user where you can share you space that contains all the analysis steps you did along with relevant information.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend
Comments for RSP_0536
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Gene Help

Overview

Gene landing pages present genomic, functional, and regulatory information for individual genes. A circular visualization displays connections between the selected gene and genes in the same modules, with as edges drawn between the respective coordinates of the whole genome.

The gene page also lists functional ontology assignments, module membership, and motifs associated with these modules. Genes in the network inherit regulatory influences from the modules to which they belong. Therefore, the regulatory information for each gene is a collection of all regulatory influences on these modules. These are listed as a table that includes influence name, type, and target module. If the gene is a transcription factor, its target modules are also displayed in a table that provides residual values and number of genes.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend