Organism : Synechococcus elongatus PCC 7942 | Module List:
Module 140 Profile

GeneModule member RegulatorRegulator MotifMotif
Cytoscape Web
Network Help

A network view of the module is created using cytoscapeWeb and enables dynamic, interactive exploration of the module properties. In this view, module member genes, motifs, and regulatory influences are represented as peripheral nodes connected to core module node via edges.

Module members are green circles, regulators are red triangles and motifs are blue diamonds. Selection of a node gives access to detailed information in a pop-up window, which allows dragging and pinning to compare multiple selections. Selecting module members will show information about the selected gene such as name, species and fucntions. Motif selection will show motif logo image and e-values. Bicluster selction will show expression profile and summary statistics for the module.

GeneModule member RegulatorRegulator MotifMotif
Regulators for Module 140

There are 0 regulatory influences for Module 140

Warning: No Regulators were found!

Regulator Help

For each module, single or AND logic connected regulatory influences are listed under the regulators tab. These regulatory influences are identified by Inferelator. Table shows name of the regulator and its type.

tf: Transcription factor

ef: Environmental factor

combiner: Combinatorial influence of a tf or an ef through logic gate. Table is sortable by clicking on the arrows next to column headers.

Motif information (de novo identified motifs for modules)

There are 3 motifs predicted.

Motif Table (3)
Motif Id e-value Consensus Motif Logo
1937 2.70e+01 GCctT.cTgGcAAgagctGatTg
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1938 1.50e+04 ctGTcacccaaaTtgCtGtcG
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1939 3.00e+05 gtTtGcCGC.agTtT
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Motif Help

Transcription factor binding motifs help to elucidate regulatory mechanism. cMonkey integrates powerful de novo motif detection to identify conditionally co-regulated sets of genes. De novo predicted motifs for each module are listed in the module page as motif logo images along with associated prediction statistics (e-values). The main module page also shows the location of these motifs within the upstream sequences of the module member genes.

Motifs of interest can be broadcasted to RegPredict (currently only available for Desulfovibrio vulgaris Hildenborough) in order to compare conservation in similar species. This integrated motif prediction and comparative analysis provides an additional checkpoint for regulatory motif prediction confidence.

Motif e-value: cMonkey tries to identify two motifs per modules in the upstream sequences of the module member genes. Motif e-value is an indicative of the motif co-occurences between the members of the module.Smaller e-values are indicative of significant sequence motifs. Our experience showed that e-values smaller than 10 are generally indicative of significant motifs.

Functional Enrichment

Regulon 140 is enriched for following functions.

TIGRFam Enrichment Table

Function Name Function Type Unadjusted pvalue Benjamini& Hochberg pvalue Genes with function Method
Biosynthesis of cofactors, prosthetic groups, and carriers tigr mainrole 4.09e-04 8.79e-03 5/28
Biosynthesis of cofactors, prosthetic groups, and carriers tigr mainrole 4.09e-04 8.62e-04 5/28

COG Enrichment Table

Function Name Function Type Unadjusted pvalue Benjamini& Hochberg pvalue Genes with function Method
Transcription cog subcategory 1.88e-03 3.66e-02 4/28
Posttranslational modification, protein turnover, chaperones cog subcategory 4.09e-04 1.37e-02 5/28
Information storage and processing cog category 1.33e-02 2.25e-02 7/28
Cellular processes and signaling cog category 6.58e-03 1.16e-02 9/28
Transcription cog subcategory 1.88e-03 3.52e-03 4/28
Signal transduction mechanisms cog subcategory 2.76e-02 4.42e-02 3/28
Posttranslational modification, protein turnover, chaperones cog subcategory 4.09e-04 8.47e-04 5/28
Coenzyme transport and metabolism cog subcategory 1.07e-02 1.83e-02 4/28
Functions Help

Biological networks contain sets of regulatory units called functional modules that together play a role in regulation of specific functional processes. Connections between different modules in the network can help identify regulatory relationships such as hierarchy and epistasis. In addition, associating functions with modules enables putative assignment of functions to hypothetical genes. It is therefore essential to identify functional enrichment of modules within the regulatory network.

Functional annotations from single sources are often either not available or not complete. Therefore, we integrated KEGG pathway, Gene Ontology, TIGRFam and COG information as references for functional enrichment analysis.

We use hypergeometric p-values to identify significant overlaps between co-regulated module members and genes assigned to a particular functional annotation category. P-values are corrected for multiple comparisons by using Benjamini-Hochberg correction and filtered for p-values ≤ 0.05.

Network Portal presents functional ontologies from KEGG, GO, TIGRFAM, and COG as separate tables that include function name, type, corrected and uncorrected hypergeometric p-values, and the number of genes assigned to this category out of total number of genes in the module.

Members for Module 140

There are 28 genes in Module 140

Gene Member Table (28)
Name Common name Type Gene ID Chromosome Start End Strand Description TF
Synpcc7942_0395 CDS 3774916 chromosome 388494 389618 + hypothetical protein False
Synpcc7942_0576 thiG CDS 3774814 chromosome 557200 558060 + thiazole-phosphate synthase (IMGterm) False
Synpcc7942_0600 CDS 3775318 chromosome 588528 590060 - serine/threonine protein kinase (IMGterm) False
Synpcc7942_0625 CDS 3775608 chromosome 616419 618185 + Single-stranded nucleic acid binding R3H False
Synpcc7942_0713 ispG CDS 3775884 chromosome 706450 707673 - 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (EC 1.17.4.3) (IMGterm) False
Synpcc7942_0771 CDS 3775948 chromosome 763640 764854 - hypothetical protein (EC:2.5.1.18) False
Synpcc7942_0878 CDS 3774055 chromosome 883835 886261 + "ribonuclease, Rne/Rng family (EC:3.1.26.12)" False
Synpcc7942_0879 CDS 3774056 chromosome 886245 886892 + RNase HII (EC 3.1.26.4) (IMGterm) False
Synpcc7942_1096 CDS 3775046 chromosome 1113354 1114724 - hydroxymethylpyrimidine synthase (IMGterm) False
Synpcc7942_1124 CDS 3775074 chromosome 1141288 1143300 + Exoribonuclease II (EC:3.1.13.1) False
Synpcc7942_1331 CDS 3775524 chromosome 1363290 1363466 + CAB/ELIP/HLIP superfamily protein False
Synpcc7942_1332 CDS 3775525 chromosome 1363471 1364091 + hypothetical protein False
Synpcc7942_1395 CDS 3774108 chromosome 1445306 1445725 + hypothetical protein False
Synpcc7942_1404 CDS 3774117 chromosome 1455395 1456084 - "two component transcriptional regulator, winged helix family (IMGterm)" True
Synpcc7942_1417 CDS 3775215 chromosome 1471000 1471983 + hypothetical protein False
Synpcc7942_1496 CDS 3773669 chromosome 1546647 1547552 - N-acetylglutamate kinase (EC 2.7.2.8) (IMGterm) False
Synpcc7942_1497 CDS 3773670 chromosome 1547545 1548105 - hypothetical protein False
Synpcc7942_1527 CDS 3774951 chromosome 1586243 1588759 + nitrogen assimilation regulatory protein True
Synpcc7942_1597 folE CDS 3775021 chromosome 1663657 1664298 + GTP cyclohydrolase I (EC 3.5.4.16) (IMGterm) False
Synpcc7942_1598 CDS 3775022 chromosome 1664306 1665100 + Phenazine biosynthesis PhzC/PhzF protein False
Synpcc7942_1983 CDS 3774170 chromosome 2052981 2054405 + three-step phytoene desaturase (EC 1.3.99.-) / zeta-carotene desaturase (EC 1.3.99.-) (IMGterm) False
Synpcc7942_2109 CDS 3774328 chromosome 2189141 2190916 + ATPase False
Synpcc7942_2273 CDS 3774555 chromosome 2340153 2340920 + hypothetical protein False
Synpcc7942_2324 CDS 3774607 chromosome 2391369 2392340 + glutathione synthase (EC 6.3.2.3) (IMGterm) False
Synpcc7942_2407 CDS 3774691 chromosome 2480159 2480938 + hypothetical protein False
Synpcc7942_2449 CDS 3774468 chromosome 2526169 2526804 + 1-Cys peroxiredoxin (EC 1.11.1.15) (IMGterm) False
Synpcc7942_2524 tig CDS 3775116 chromosome 2605278 2606702 + trigger factor False
Synpcc7942_2526 clpX CDS 3775118 chromosome 2607529 2608878 + ATP-dependent Clp protease ATP-binding subunit ClpX (EC 3.4.21.92) (IMGterm) False

Genes Help

Gene member table shows all the genes included in the module. Listed attributes are;

  1. Name: Gene name or Locus tag
  2. Common Name: Gene short name
  3. Type: Type of the feature, usually CDS.
  4. Gene ID: Link to NCBI Gene ID
  5. Chromosome: Chromosome name from annotation file
  6. Start/End:Feature start and end coordinates
  7. Strand: strand of the gene
  8. Description: Description of the gene from annotation file
  9. TF: If the gene is a Transcription Factor or not.

If you are browsing the Network Portal by using Gaggle/Firegoose, firegoose plugin will capture the NameList of the gene members. Captured names can be saved into your Workspace by clicking on "Capture" in the firegoose toolbar or can be directly sent other desktop and web resources by using "Broadcast" option.

Comments for Module 140

Please add your comments for this module by using the form below. Your comments will be publicly available.

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Social Help

You can start a conversation about this module or join the existing discussion by adding your comments. In order to be able to add your comments you need to sign in by using any of the following services;Disqus, Google, Facebook or Twitter. For full compatibility with other network portal features, we recommend using your Google ID.

Help

What is a module?

Regulatory units (modules) in the Network Portal are based on the network inference algorithm used. For the current version, modules are based on cMonkey modules and Inferelator regulatory influences on these modules. More specifically, module refers to set of genes that are conditionally co-regulated under subset of the conditions. Identification of modules integrates co-expression, de-novo motif identification, and other functional associations such as operon information and protein-protein interactions.

Module Overview

The landing module page shows quick summary info including co-expression profiles, de-novo identified motifs, and transcription factors and/or environmental factors as regulatory influences. It also includes module residual, motif e-values, conditions and links to other resources such as NCBI and Microbesonline. . If a transcription factor is included in the manually curated RegPrecise database, further information from RegPrecise is shown, allowing users to perform comparative analysis.

Expression Profiles

Expression profiles is a plot of the expression ratios (log10) of the module's genes, over all subset of the conditions included in the module. The X-axis represent conditions and the Y-axis represents log10 expression ratios. Each gene is plotted as line plot with different colors. Colored legend for the lines are presented under the plot. This plot is dynamic. Clicking on the gene names in the legend will show/hide the plot for that particular gene. A tooltip will show expression ratio information if you mouseover the lines in the plot.

Motif Locations

Location of the Identified motifs for the module in the upstream regions of the member genes are shown under the expression profiles plot. This plot shows the diagram of the upstream positions of the motifs, colored red and green for motifs #1, and 2, respectively. Intensity of the color is proportional to the significance of the occurence of that motif at a given location. Motifs on the forward and reverse strand are represented over and under the line respectively.

Network

A network view of the module is created using cytoscapeWeb and enables dynamic, interactive exploration of the module properties. In this view, module member genes, motifs, and regulatory influences are represented as peripheral nodes connected to core module node via edges. Module members are green circles, regulators are red triangles and motifs are blue diamonds. Selection of a node gives access to detailed information in a pop-up window, which allows dragging and pinning to compare multiple selections. Selecting module members will show information about the selected gene such as name, species and fucntions. Motif selection will show motif logo image and e-values. Bicluster selction will show expression profile and summary statistics for the module.

GeneModule member RegulatorRegulator MotifMotif

Regulators

For each module, single or AND logic connected regulatory influences are listed under the regulators tab. These regulatory influences are identified by Inferelator. Table shows name of the regulator and its type. tf: Transcription factor, ef: Environmental factor and combiner:Combinatorial influence of a tf or an ef through logic gate. Tabel is sortable by clicking on the arrows next to column headers.

Motifs

Transcription factor binding motifs help to elucidate regulatory mechanism. cMonkey integrates powerful de novo motif detection to identify conditionally co-regulated sets of genes. De novo predicted motifs for each module are listed in the module page as motif logo images along with associated prediction statistics (e-values). The main module page also shows the location of these motifs within the upstream sequences of the module member genes.

Motifs of interest can be broadcasted to RegPredict (currently only available for Desulfovibrio vulgaris Hildenborough) in order to compare conservation in similar species. This integrated motif prediction and comparative analysis provides an additional checkpoint for regulatory motif prediction confidence.

Functions

Biological networks contain sets of regulatory units called functional modules that together play a role in regulation of specific functional processes. Connections between different modules in the network can help identify regulatory relationships such as hierarchy and epistasis. In addition, associating functions with modules enables putative assignment of functions to hypothetical genes. It is therefore essential to identify functional enrichment of modules within the regulatory network.

Functional annotations from single sources are often either not available or not complete. Therefore, we integrated KEGG pathway, Gene Ontology, TIGRFam and COG information as references for functional enrichment analysis.

We use hypergeometric p-values to identify significant overlaps between co-regulated module members and genes assigned to a particular functional annotation category. P-values are corrected for multiple comparisons by using Benjamini-Hochberg correction and filtered for p-values ≤ 0.05.

Network Portal presents functional ontologies from KEGG, GO, TIGRFAM, and COG as separate tables that include function name, type, corrected and uncorrected hypergeometric p-values, and the number of genes assigned to this category out of total number of genes in the module.

Genes

Gene member table shows all the genes included in the module. Listed attributes are;

  1. Name: Gene name or Locus tag
  2. Common Name: Gene short name
  3. Type: Type of the feature, usually CDS.
  4. Gene ID: Link to NCBI Gene ID
  5. Chromosome: Chromosome name from annotation file
  6. Start/End:Feature start and end coordinates
  7. Strand: strand of the gene
  8. Description: Description of the gene from annotation file
  9. TF: If the gene is a Transcription Factor or not.

If you are browsing the Network Portal by using Gaggle/Firegoose, firegoose plugin will capture the NameList of the gene members. Captured names can be saved into your Workspace by clicking on "Capture" in the firegoose toolbar or can be directly sent other desktop and web resources by using "Broadcast" option.

Social

You can start a conversation about this module or join the existing discussion by adding your comments. In order to be able to add your comments you need to sign in by using any of the following services;Disqus, Google, Facebook or Twitter. For full compatibility with other network portal features, we recommend using your Google ID.

Definitions

Residual: is a measure of bicluster quality. Mean bicluster residual is smaller when the expression profile of the genes in the module is "tighter". So smaller residuals are usually indicative of better bicluster quality.

Expression Profile: is a preview of the expression profiles of all the genes under subset of conditions included in the module. Tighter expression profiles are usually indicative of better bicluster quality.

Motif e-value: cMonkey tries to identify two motifs per modules in the upstream sequences of the module member genes. Motif e-value is an indicative of the motif co-occurences between the members of the module.Smaller e-values are indicative of significant sequence motifs. Our experience showed that e-values smaller than 10 are generally indicative of significant motifs.

Genes: Number of genes included in the module.

Functions: We identify functional enrichment of each module by camparing to different functional categories such as KEGG, COG, GO etc. by using hypergeometric function. If the module is significantly enriched for any of the functions, this column will list few of the these functions as an overview. Full list of functions is available upon visiting the module page under the Functions tab.